Paper Type |
Contributed Paper |
Title |
Potential of L-phenylalanine Production from Raw Glycerol of Palm Biodiesel Process by a Recombinant Escherichia coli |
Author |
Penjit Srinophakun, Supachai Reakasame, Methee Khamduang, Kanoktip Packdibamrung and Anusith Thanapi |
Email |
fengjrc@ku.ac.th |
Abstract: The possibility of using raw glycerol from biodiesel process for the bacteria growth and L-phenylalanine production using recombinant Escherichia coli BL21(DE3) was investigated. Raw glycerol with 75.50% (w/w) glycerol content was pretreated with concentrated sulfuric acid to reduce the impurities. Type A and type B glycerol containing 91.28% and 83.93% glycerol content were obtained after the acid pretreatment. Raw glycerol, Type A and type B glycerol and commercial grade glycerol (99.5%) at various concentrations (5, 10, 30 and 50 g/L) were used as a carbon source. The cell dry weight (CDW) and L-phenylalanine concentration obtained after 36 h of cultivation from raw glycerol were much higher than that from other types of glycerol. The highest CDW (3.47 g/L) and L-phenylalanine (55.2 mg/L) were obtained from the medium containing 30 g/L of raw glycerol. The results indicated that raw glycerol from biodiesel process was an interesting carbon source for L-phenylalanine production. |
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Start & End Page |
59 - 68 |
Received Date |
2010-12-14 |
Revised Date |
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Accepted Date |
2011-07-14 |
Full Text |
Download |
Keyword |
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Volume |
Vol.39 No.1 (JANUARY 2012) |
DOI |
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Citation |
Srinophakun P., Reakasame S., Khamduang M., Packdibamrung K. and Thanapi A., Potential of L-phenylalanine Production from Raw Glycerol of Palm Biodiesel Process by a Recombinant Escherichia coli, Chiang Mai J. Sci., 2012; 39(1): 59-68. |
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