Chiang Mai Journal of Science

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The production of carotenoids pigment was studied by culturing Rhodotorula glutinis mutant (mB34) in yeast-malt extract medium in fermenter. The mB34 strain was cultivated at various concentrations of ingredients of yeast-malt extract broth containing 0.1, 0.3, 0.5 and 1.0 % (w/v) yeast extract, 0.05, 0.1, 0.3 and 0.5 % (w/v) malt extract, 0.2, 0.3, 0.4 and 0.5 % (w/v) peptone and 1, 3, 5 and 7 % (w/v) glucose at initial pH 5.0 in fermenter working volume 1.5 litres with aeration rate 1.5 vvm and stirrer speed 300 rpm. The yeast mB34 was harvested and carotenoids were extracted and analyzed by uv-visible spectrophotometry. Carotenoids content was determined at maximum absorbance 474 nm. It was found that the mB34 grew well and produced higher carotenoids pigment in yeast-malt extract medium containing 0.3 % (w/v) yeast extract, 0.3 % (w/v) malt extract, 0.5 %(w/v) peptone and 3 % (w/v) glucose than other concentrations. The total pigments of mB34 and dry cell mass was 92.52 mg/g of yeast and 9.80 g/l, respectively at 72 h.

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