Use of Mustard Meal Media as a Substrate for Astaxanthin Production by Xanthophyllomyces dendrorhous
Raviwan Tinoi [a], Nuansri Rakariyatham* [a] and Richard L. Deming [b]* Author for corresponding; e-mail address: nuansri1@yahoo.com
Volume :Vol.31 No.3 September 2004
Research Article
DOI:
Received: 6 January 2004, Revised: -, Accepted: 20 August 2004, Published: -
Citation: Tinoi R., Rakariyatham N. and Deming R.L., Use of Mustard Meal Media as a Substrate for Astaxanthin Production by Xanthophyllomyces dendrorhous, Chiang Mai Journal of Science, 2004; 31(3): 293-302.
Abstract
Mustard meal after seed-oil expellation was used as a substrate for growth of Xanthophyllomyces dendrorhous and production of astaxanthin. The mustard meal media that provided both sugar and nitrogen sources were prepared by boiling in water (un-hydrolyzed mustard meal media) and hydrolyzing in acid (hydrolyzed mustard meal media). The red yeast X. dendrorhous grew best in the un-hydrolyzed mustard meal media and produced commercially valuable carotenoid pigments, especially astaxanthin. The maximum yield of cell dry weight (6.25 g/L), total carotenoid (4.17 mg/L) and astaxanthin (3.83 mg/L) were obtained with optimal process parameters of 20% un-hydrolyzed mustard meal media, an initial pH of medium at 5.0, an incubation temperature of 20 C and a cultivation time of 120 h.