Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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Genetical Diversity of Mastigocladus in Ranong Hot Spring, Southern Part of Thailand

Udomluk Sompong* [a], Richard W. Castenholz [b], Somboon Anuntalabhochai [a] and Yuwadee Peerapornp
* Author for corresponding; e-mail address: s_udomluk@yahoo.com
Volume: Vol.33 No.3 (SEPTEMBER 2006)
Research Article
DOI:
Received: -, Revised: -, Accepted: -, Published: -

Citation: Sompong U., Castenholz R.W. and Peerapornp S.A..A., Genetical Diversity of Mastigocladus in Ranong Hot Spring, Southern Part of Thailand, Chiang Mai Journal of Science, 2006; 33(3): 363-370.

Abstract

Among photosynthetic prokaryotes, the cyanobacteria represent by far the largest and most diverse group. Mastigocladus is one of the thermophilic species found in hot springs throughout the world including Thailand. Mastigocladus mats occur dominant in Ranong Hot Spring, Ranong Province, Southern Thailand. Cyanobacterial mats have been studied in 40-60 ºC of temperature intervals. They were observed by using microscopy and molecular technique analysis. In this study, the denaturing gradient gel electrophoresis (DGGE) analysis of PCRamplified 16S rDNA gene segments was used to profile cyanobacterial populations inhabiting different temperature regions in the mat community. From this technique, Mastigocladus - like cyanobacteria were found in every temperature range and every season, however there was no significant difference between cyanobacterial populations in the Ranong Hot Spring.

Keywords: Mastigocladus, thermophilic cyanobacteria, DGGE, hot spring, Thailand

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