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Gas Chromatographic-Flame Photometric Detection of Organophosphate Pesticide Residues and Its Application in Real Vegetable and Fruit Samples from Chiang Mai City, Thailand


Paper Type 
Contributed Paper
Title 
Gas Chromatographic-Flame Photometric Detection of Organophosphate Pesticide Residues and Its Application in Real Vegetable and Fruit Samples from Chiang Mai City, Thailand
Author 
Watcharapol Polyiem, Warangkana Naksen and Tippawan Prapamontol
Email 
tippawan.prapamontol@cmu.ac.th
Abstract:
 

Analysis of pesticide residues is usually limited by expensive analytical apparatus and trained laboratory scientist. We developed a method for detecting 20 organophosphate (OP) pesticide residues in vegetable and fruit matrices using gas chromatographic- flame photometric detection. OP pesticide residues were extracted from well-homogenized vegetable or fruit samples using acetonitrile and clean-up with graphite carbon black solid phase extraction. The clean OP residues’ extract was finally dissolved in ethyl acetate and analyzed by GC-FPD.  Mean recovery (±SD) of 20 OP pesticides was 92.9 (±10.8) % and individual mean ranged from 76.8 % (methamidophos) to 114 % (triazophos). Limits of detection of 20 OP pesticides ranged from 0.0003 mg/kg (diazinon) to 0.015 mg/kg (azinphos-methyl) which are well below the Codex maximum residue limits (MRLs). In addition, determination of 20 OP pesticides in the present method has proven to be suitable for detecting OP residues in Thailand food safety policy since major imported active OP pesticides were already covered.

Start & End Page 
1933 - 1943
Received Date 
2017-05-26
Revised Date 
Accepted Date 
2017-12-07
Full Text 
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Keyword 
Organophosphate pesticides, gas chromatographic-flame photometric detection, vegetables, fruits, Thailand
Volume 
Vol.45 No.4 (July 2018)
DOI 
Citation 
Polyiem W., Naksen W. and Prapamontol T., Gas Chromatographic-Flame Photometric Detection of Organophosphate Pesticide Residues and Its Application in Real Vegetable and Fruit Samples from Chiang Mai City, Thailand, Chiang Mai J. Sci., 2018; 45(4): 1933-1943.
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