No Session
Chiang Mai Journal of Science, Faculty of Science, Chiang Mai University
 







1.0
2023CiteScore
 
33rd percentile
Powered by  Scopus

SCImago Journal & Country Rank












Journal Volumes


Submit your paper
Download Template

  JOURNAL DETAIL



Determination of Local Tobacco Cultivars Using ISSR Molecular Marker


Paper Type 
 Contributed Paper
Title 
 Determination of Local Tobacco Cultivars Using ISSR Molecular Marker
Author 
 Jessada Denduangboripant, Sornsuda Setaphan, Wilasinee Suwanprasart, and Somsak Panha
Email 
 jessada.d@chula.ac.th
Abstract:
In many tobacco-growing countries, imported and local tobacco cultivars are subject to different regulations in tariff collection. However, there is a major technical problem how to distinguish between the imported and local cultivars. No standard method has been established to judge whether the plant has been grown for long time, enough to be legally called “local cultivar”. In this study, an Inter-Simple Sequence Repeat (ISSR) method was introduced to study genetic relationships between imported and local tobacco cultivars grown in Thailand. Of 20 screened-primers, five ISSR primers showed polymorphic PCR amplified
products suitable for further phylogenetic analysis. Among them, two primers generated specific PCR bands that could separate local tobacco cultivars (Chorlare 1 and Chorlare 2) from other imported cultivars. Genetic relationship trees revealed four pairs of tobacco cultivars having bootstrap supporting-values higher than 50%. Among all 13 local tobacco cultivars collected and examined, only the couple of Chorlare 1 and 2 was distantly separated from the imported cultivars, suggesting their long-history of being grown in Thailand. The other 11 local cultivars were found to be morphologically and genetically similar to some imported cultivars and could have originated from them. Therefore, the ISSR technique could be developed and implemented as a simple, effective molecular marker to recognise early-introduced local tobacco cultivars.
Start & End Page 
 293 - 303
Received Date 
 2009-10-19
Revised Date 
Accepted Date 
 2009-12-11
Full Text 
   Download
Keyword 
 inter-simple sequence repeat, ISSR, local cultivar, molecular marker, Thailand, tobacco
Volume 
 Vol.37 No.2 (MAY 2010)
DOI 
Citation 
 Denduangboripant J., Setaphan S., Suwanprasart W., and Panha S., Determination of Local Tobacco Cultivars Using ISSR Molecular Marker , Chiang Mai Journal of Science, 2010; 37(2): 293-303.
SDGs
View:664 Download:312

  RELATED ARTICLE

Diversity of Cultivable Bacterial Populations Provides Evidence for Tobacco-crop Rotations in Alleviating Continuous Cropping Obstacles
Article ID: e2025020
Author:Xin Liu, Yijie Zhang, Yan Chen, Biao He, Zhufeng Shi, Tianhua Han, Feifei He, You Luo, Zhuli Shi, Yu Wang, Xuewei Li and Peiwen Yang
Vol.52 No.2 (March 2025) View: 123 Download:68
Soil Physicochemical Properties and Rhizosphere Microorganisms Mediates the Growth of Cigar Tobacco Across Different Ecoregions in Yunnan, Southwest China
Article ID: e2024047
Author:Junru Shen,Yijie Zhang, Guoyou He, Bao Fang, Longhui Ren, Anzhong Zhen, Xubing Tang, Tao Yang and Chuisi Kong
Vol.51 No.3 (May 2024) View: 495 Download:288
Future Projections of Precipitation and Temperature in Northeast, Thailand using Bias-Corrected Global Climate Models
Article ID: e2023041
Author:Wittanan Tammadid, Ketmanee Nantasom, Wichayaporn Sirksiri, Supika Vanitchung, Chaiwatchara Promjittiphong, Atsamon Limsakul and Phongthep Hanpattanakit
Vol.50 No.4 (July 2023) View: 1,060 Download:935
Different Levels of Nitrogen Fertilizer in Nursery Stage Positively Affect the Activity of Defense-related Enzymes and Resistance of Tobacco Plant to Phytophthora nicotianae
page: 551 - 554
Author:Ruifang Song, Waqar Ahmed, Yujiao Tan and Zhengxiong Zhao
Vol.49 No.3 (Special Issue II : May 2022) View: 1,430 Download:461
https://gmsmushrooms.org/: A comprehensive Online Database of Mushrooms in the Greater Mekong Sub Region
page: 538 - 550
Author:Monthien Phonemany, Allen Grace T. Niego, Samantha C. Karunarathna, Yuwei Hu, Thatsanee Luangharn, Phongeun Sysouphanthong, Saisamorn Lumyong, Rui-Lin Zhao, Mao-Qiang He, Naritsada Thongklang and Kevin D. Hyde
Vol.49 No.3 (Special Issue II : May 2022) View: 1,607 Download:689
SDGs:
Genetic Diversity of Thai Upland Rice Germplasm Based on Inter-Simple Sequence Repeats Marker
page: 1287 - 1300
Author:Kajonphol Tanaporn, Nontakod Sujaree and Sangsiri Chontira
Vol.48 No.5 (September 2021) View: 1,321 Download:375
The sidA Gene of Aureobasidium thailandense and its Phylogenetic Relationship among those of Aureobasidium Species
page: 13 - 26
Author:Vorawat Kitiyanant, Chompoonik Kanchanabanca, Hunsa Punnapayak, Anchittha Satjarak, Pongtharin Lotrakul* and Sehanat Prasongsuk*
Vol.48 No.1 (January 2021) View: 805 Download:514
Water Footprint and Virtual Water Flow of Cassava Starch of Thailand
page: 1129 - 1142
Author:Manuswee Phanichnok, Khanidtha Meevasana and Pongthep Suwanwaree*
Vol.46 No.6 (November 2019) View: 978 Download:1,096
Genetic Relationships Between Globba expansa (Zingiberaceae) and Other Closely Related Taxa in Thailand Using HAT-RAPD Marker Analysis
page: 896 - 906
Author:Sunisa Sangvirotjanapat, Pramote Triboun, Mark Newman and Jessada Denduangboriphant*
Vol.46 No.5 (September 2019) View: 748 Download:544
Assessment and Ranking of Hot Springs Sites Representing Geothermal Resources in Southern Thailand using Positive Attitude Factors
page: 592 - 608
Author:Wipada Ngansom and Helmut Duerrast *
Vol.46 No.3 (May 2019) View: 1,255 Download:326
Development of Recommendations for Site Investigations of waste Disposal Sites in Thailand
page: 235 - 245
Author:Jens Radschinski* and Hans-Jürgen Voigt
Vol.30 No.3 (DECEMBER 2003) View: 654 Download:268
Investigation of Plasmodium vivax Rhomboid-like Protease 1 Compared to Plasmodium falciparum Rhomboid Protease 1 in Erythrocytic Cycle
page: 2256 - 2266
Author:Touchchapol Mataradchakul, Chairat Uthaipibull, Joel Vega-Rodriguez, Marcelo Jacobs-Lorena and Usa Lek-Uthai
Vol.45 No.6 (September 2018) View: 773 Download:231
Gas Chromatographic-Flame Photometric Detection of Organophosphate Pesticide Residues and Its Application in Real Vegetable and Fruit Samples from Chiang Mai City, Thailand
page: 1933 - 1943
Author:Watcharapol Polyiem, Warangkana Naksen and Tippawan Prapamontol
Vol.45 No.4 (July 2018) View: 1,037 Download:273
Inter-Simple Sequence Repeat Markers Reveal Genetic Relatedness Between Natural Aquilaria Populations in Peninsular Malaysia
page: 1307 - 1317
Author:Shiou Yih Lee, Wei Lun Ng, Dhilia Udie Lamasudin and Rozi Mohamed*
Vol.45 No.3 (May 2018) View: 714 Download:323
First Record of Platybaetis bishopi Mller-Liebenau, 1980 and Baetiella bispinosa (Gose, 1980) (Ephemeroptera: Baetidae) from Thailand
page: 774 - 783
Author:Sirikamon Phlai-ngam and Nisarat Tungpairojwong*
Vol.45 No.2 (March 2018) View: 685 Download:524
Crustal Structures, Geothermal Sources and Pathways Beneath Northern Thailand Revealed by Local Earthquake Tomography
page: 565 - 575
Author:Kasemsak Saetang, Wilaiwan Srisawat and Helmut Dürrast
Vol.45 No.1 (January 2018) View: 773 Download:521
Two New Records of Lichens of Thelotremoid Graphidaceae (Ascomycota: Ostropales) from Thailand
page: 1392 - 1394
Author:Khwanyuruan Naksuwankul* and H. Thorsten Lumbsch
Vol.44 No.4 (October 2017) View: 718 Download:198
Genetic Divergence of Austroasiatic Speaking Groups in the Northeast of Thailand: A Case Study on Northern Khmer and Kuy
page: 1279 - 1294
Author:Panthipa Chantakot, Pittayawat Pittayaporny, Kanokpohn Srithongdaeng, Suparat Srithawong and Wibhu
Vol.44 No.4 (October 2017) View: 700 Download:456
Tectonic Evolution of the Thonburi Basin in the Lower Central Plain, Thailand
page: 1306 - 1315
Author:Kamontip Nuangchamnong [a], Phumee Srisuwon [b] and Passakorn Pananont* [a]
Vol.43 No.6 (SPECIAL ISSUE 2) View: 712 Download:374
Shear wave velocity estimation of the near-surface sediments of Bangkok and vicinity, Thailand for seismic site characterization
page: 1269 - 1278
Author:Aomboon Naksawee [a], Koich Hayashi [b] and Passakorn Pananont* [a]
Vol.43 No.6 (SPECIAL ISSUE 2) View: 656 Download:398
Observations of Cloud Condensation Nuclei (CCN) in Northern Thailand
page: 767 - 776
Author:Thitikorn Chanyatham*, Pakdee Chantraket and Nuenghatai Tantiplubthong
Vol.43 No.4 (JULY 2016) View: 1,187 Download:261
The Genus Butea Roxb. ex Willd. (Leguminosae-Papilionoideae) in Thailand
page: 367 - 375
Author:Sakuntala Ninkaew and Pranom Chantaranothai
Vol.42 No.2 (APRIL 2015) View: 680 Download:346
A Comparative Study of Genetic Diversity in Chickpea Based Upon Touchdown and Non-touchdown PCR Using ISSR Markers
page: 117 - 125
Author:Mahesh Kumar, Vinod Chhokar, Anil Kumar, Sarla, Vikas Beniwal and Himanshu Aggarwal
Vol.42 No.1 (JANUARY 2015) View: 1,104 Download:179
Betel-like-scented Piper Plants as Diverse Sources of Industrial and Medicinal Aromatic Chemicals
page: 1171 - 1181
Author:Arisa Sanubol [a], Arunrat Chaveerach*[a], Runglawan Sudmoon [a], Tawatchai Tanee [b], Kowit Noikotr
Vol.41 No.5/1 (OCTOBER 2014) View: 1,323 Download:260
A New Species of Bactrachospermum, B. khaoluangensis Chankaew, Peerapornpisal et Kumano, sp. Nov. (Batrachospermales, Rhodophyta) from Thailand
page: 1 - 10
Author:Wanninee Chankaew*[a,b], Yuwadee Peerapornpisal[c], Shigeru Kumano[d] and Chatchai Ngamriabsakul[a]
Vol.40 No.1 (JANUARY 2013) View: 656 Download:254
Microstructures in Pyrope Garnets from Thailand
page: 669 - 677
Author:Bongkot Phichaikamjornwut, Henrik Skogby, Prayote Ounchanum, Phisit Limtrakun and Apichet Boonsoong
Vol.39 No.4 (OCTOBER 2012) View: 650 Download:252
Evaluation of Precipitation Simulations over Thailand using a WRF Regional Climate Model
page: 623 - 638
Author:Chakrit Chotamonsak *[a], Eric P. Salathé Jr. [b], Jiemjai Kreasuwan [c] and Somporn Chantara [a]
Vol.39 No.4 (OCTOBER 2012) View: 687 Download:236
Diversity of Edible Cladophora (Cladophorales, Chlorophyta) in Northern and Northeastern Thailand, Based on Morphology and Nuclear Ribosomal DNA Sequences
page: 300 - 310
Author:Sorrachat Thiamdao* [a], Ga Hun Boo [b], Sung Min Boo [b] and Yuwadee Peerapornpisal [a, c]
Vol.39 No.2 (APRIL 2012) View: 2,183 Download:194
Nostochopsis lobatus Wood em. Geitler (Nostocales), Edible Algae in Northern Thailand
page: 119 - 127
Author:Sorrachat Thiamdao, Manita Motham, Jeeraporn Pekkoh, Lapatrada Mungmai and Yuwadee Peerapornpisal
Vol.39 No.1 (JANUARY 2012) View: 701 Download:210
Water Quality and Trophic Status in Main Rivers of Thailand
page: 280 - 294
Author:Pongpan Leelahakriengkrai*[a] and Yuwadee Peerapornpisal [a,b]
Vol.38 No.2 (APRIL 2011) View: 663 Download:419
Genetical Diversity of Mastigocladus in Ranong Hot Spring, Southern Part of Thailand
page: 363 - 370
Author:Udomluk Sompong* [a], Richard W. Castenholz [b], Somboon Anuntalabhochai [a] and Yuwadee Peerapornp
Vol.33 No.3 (SEPTEMBER 2006) View: 1,229 Download:262
Microcystin LR Content in Microcystis aeruginosa Kütz Collected from Sri Sakhett, Thailand
page: 231 - 236
Author:Waya Sengpracha [a], Napattarapong Suvannachai [b] and Weerachai Phutdhawong* [b]
Vol.33 No.2 (MAY 2006) View: 752 Download:248
Fabrication and Characterization of High Strength Dental Porcelain Nanocomposite from Thailandís Raw Materials
page: 549 - 553
Author:Attavit Pisitanusorn*[a], Supon Ananta [a], Rattikorn Yimnirun [a] and Sakdipown Thiansem [b]
Vol.32 No.3 (SEPTEMBER 2005) View: 643 Download:193
Fifty Three New Record Species of Benthic Diatoms from Mekong River and Its Tributaries in Thailand
page: 326 - 343
Author:Sutthawan Suphan, and Yuwadee Peerapornpisal
Vol.37 No.2 (MAY 2010) View: 659 Download:205



Search in this journal


Document Search


Author Search

A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z

Popular Search






Chiang Mai Journal of Science

Faculty of Science, Chiang Mai University
239 Huaykaew Road, Tumbol Suthep, Amphur Muang, Chiang Mai 50200 THAILAND
Tel: +6653-943-467




Faculty of Science,
Chiang Mai University




EMAIL
cmjs@cmu.ac.th




Copyrights © Since 2021 All Rights Reserved by Chiang Mai Journal of Science