Comparative Chloroplast Genome Analysis of Bupleurum sikangense and Its Allied Species Revealed An Efficient SCAR Marker For Its Identification
Kaiying Ge, Fang Li, Xuena Xie, Yanfengyang Jiang, Xiaoting Yang, Zhiye Yang, Yingyi Tan and Zhi Chao* Author for corresponding; e-mail address: chaozhi@smu.edu.cn
Volume: Vol.52 No.3 (May 2025)
Research Article
DOI: https://doi.org/10.12982/CMJS.2025.030
Received: 27 November 2024, Revised: 3 March 2025, Accepted: 26 March 2025, Published: 16 May 2025
Citation: Ge K., Li F., Xie X., Jiang Y., Yang X., Yang Z., et al., Comparative Chloroplast Genome Analysis of Bupleurum sikangense and Its Allied Species Revealed An Efficient SCAR Marker For Its Identification, Chiang Mai Journal of Science, 2025; 52(3): e2025030. DOI 10.12982/CMJS.2025.030.
Abstract
Bupleurum sikangense, a new species discovered in 2011, is abundant in the Hengduan Mountains of China, and has the potential for a new origin of the crude drug Chaihu (Radix Bupleuri). A convenient method is therefore desired to differentiate it from the allied species. In this study, we found a unique insertion (AAATATCCTACATA) in B. sikangense by comparing its whole chloroplast genome with those of the other twenty-one Bupleurum species and based upon the insertion we designed a pair of species-specific primers (sxkF/R). With the primers, we established a sequence characterized amplified region (SCAR) detection method, which reached a successful rate of 100% in identifying B. sikangense, providing a useful tool for its further exploitation.