Submit your paper 
Download Template JOURNAL DETAIL
Comparative Chloroplast Genome Analysis of Bupleurum sikangense and Its Allied Species Revealed An Efficient SCAR Marker For Its Identification
Paper Type  |
Contributed Paper |
Title |
Comparative Chloroplast Genome Analysis of Bupleurum sikangense and Its Allied Species Revealed An Efficient SCAR Marker For Its Identification |
Author |
Kaiying Ge, Fang Li, Xuena Xie, Yanfengyang Jiang, Xiaoting Yang, Zhiye Yang, Yingyi Tan and Zhi Chao |
Email |
chaozhi@smu.edu.cn |
|
Abstract: Bupleurum sikangense, a new species discovered in 2011, is abundant in the Hengduan Mountains of China, and has the potential for a new origin of the crude drug Chaihu (Radix Bupleuri). A convenient method is therefore desired to differentiate it from the allied species. In this study, we found a unique insertion (AAATATCCTACATA) in B. sikangense by comparing its whole chloroplast genome with those of the other twenty-one Bupleurum species and based upon the insertion we designed a pair of species-specific primers (sxkF/R). With the primers, we established a Sequence Characterized Amplified Region (SCAR) detection method, which reached a successful rate of 100% in identifying B. sikangense, providing a useful tool for its further exploitation. |
|
Article ID |
e2025030 |
Received Date |
2024-11-27 |
Revised Date |
2025-03-03 |
Accepted Date |
2025-03-26 |
Keyword |
Bupleurum sikangense, radix bupleuri, sequence characterized amplified region (SCAR), molecular marker, chloroplast genome |
Volume |
Vol.52 No.3 In progress (May 2025). This issue is in progress but contains articles that are final and fully citable. |
DOI |
https://doi.org/10.12982/CMJS.2025.030 |
Citation |
Ge K.,Li F.,Xie X.,Jiang Y.,Yang X.,Yang Z., et al., Comparative Chloroplast Genome Analysis of Bupleurum sikangense and Its Allied Species Revealed An Efficient SCAR Marker For Its Identification, Chiang Mai Journal of Science, 2025; 52(3): e2025030. DOI 10.12982/CMJS.2025.030. |
SDGs |
|
| View:35 Download:0 | |
RELATED ARTICLE
page: 1304 - 1310
Author:Hairul Azman Roslan*, Md. Anowar Hossain, Nur Qistina Othman, Cheksum Supiah Tawan and Isa Ipor
Vol.44 No.4 (October 2017) View: 619 Download:259
page: 293 - 303
Author:Jessada Denduangboripant, Sornsuda Setaphan, Wilasinee Suwanprasart, and Somsak Panha
Vol.37 No.2 (MAY 2010) View: 673 Download:312
Copyrights © Since 2021 All Rights Reserved by Chiang Mai Journal of Science