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A Simple Method for DNA Extraction from Activated Sludge
Paper Type  |
Contributed Paper |
Title |
A Simple Method for DNA Extraction from Activated Sludge |
Author |
Dounghatai Singka, Ladapa Kumdhitiahutsawakul, Prasert Rekkriangkrai and Wasu Pathom-aree |
Email |
wasu.p@cmu.ac.th |
|
Abstract: A protocol for DNA extraction directly from activated sludge is described. Sludge samples were lysed by glass bead and CTAB. The extracted DNA was purified with phenol: chloroform: isoamyl alcohol mixture and precipitated in sodium acetate and isopropanol. The proposed protocol is simple, rapid and required small sample size, yielding about 40.5 ± 1.8 μg DNA g-1 sludge. The quality of DNA is good enough for successful PCR amplifications of 16S rDNA, 16S rDNA V3 region and denaturing gradient gel electrophoresis (DGGE) analysis of archaeal population. This described method can also be applied to other environmental samples such as whey and soil. |
|
Start & End Page |
111 - 118 |
Received Date |
2011-07-04 |
Revised Date |
|
Accepted Date |
2011-11-04 |
Full Text |
Download |
Keyword |
Activated sludge, DNA extraction, DGGE, Archaeal population |
Volume |
Vol.39 No.1 (JANUARY 2012) |
DOI |
|
Citation |
Singka D., Kumdhitiahutsawakul L., Rekkriangkrai P. and Pathom-aree W., A Simple Method for DNA Extraction from Activated Sludge, Chiang Mai J. Sci., 2012; 39(1): 111-118. |
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