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Enhancement of Biomass, Lipid and Hydrocarbon Production from Green Microalga, Botryococcus braunii AARL G037, by UV-C Induction
Paper Type  |
Contributed Paper |
Title |
Enhancement of Biomass, Lipid and Hydrocarbon Production from Green Microalga, Botryococcus braunii AARL G037, by UV-C Induction |
Author |
Theera Thurakit, Chayakorn Pumas, Wasu Pathom-aree, Jeeraporn Pekkoh and Yuwadee Peerapornpisal |
Email |
jeeraporn.p@cmu.ac.th, yuwadee.p@cmu.ac.th |
|
Abstract: Botryococcus braunii, a green microalga, has been acknowledged as a possible source of renewable fuel that holds significant potential. However, it has been associated with a slow growth rate and low productivity. Thus, the aim of this study was to investigate the effects of ultraviolet (UV)-C on B. braunii AARL G037 for the purposes of improving biomass, lipid and hydrocarbon production levels. The survival rate was found to be in a range of 0.66-12.54% after being exposed to UV-C radiation for 3-21 minutes. The highest level of biomass production was acquired from UV-C induced isolate no. U18J10 at 2,325.67 mg L-1 and was equivalent to 1.32 times higher than the wild type. The highest hydrocarbon and lipid contents were found in B. braunii U18N14, which were recorded at 76.03% and 34.00%, respectively. Meanwhile, wild type, B. braunii AARL G037 accumulated hydrocarbons and lipids at only 45.81% and 22.70%, respectively. A study on the biodiesel properties revealed that 9-octadecanoic acid, methyl ester (C18:1) was the major fatty acid methyl ester. Moreover, biodiesel properties acquired from B. braunii wild type and all UV-C induced isolates were conformed to the EN14214 standard. |
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Start & End Page |
2637 - 2651 |
Received Date |
2018-02-14 |
Revised Date |
|
Accepted Date |
2018-04-27 |
Full Text |
Download |
Keyword |
biodiesel, fatty acid methyl ester, random mutation, renewable fuel |
Volume |
Vol.45 NO.7 (November 2018) |
DOI |
|
Citation |
Thurakit T., Pumas C., Pathom-aree W., Pekkoh J. and Peerapornpisal Y., Enhancement of Biomass, Lipid and Hydrocarbon Production from Green Microalga, Botryococcus braunii AARL G037, by UV-C Induction, Chiang Mai Journal of Science, 2018; 45(7): 2637-2651. |
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