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1, 3-Propanediol Production from Crude Glycerol by Klebsiella pneumoniae


Paper Type 
 Contributed Paper
Title 
 1, 3-Propanediol Production from Crude Glycerol by Klebsiella pneumoniae
Author 
 Jiaxin Wu*[a], Fenghuan Wang[b], Zheng Wang[b], Hua Ye[c] and Peiyi Liu[d]
Email 
 icebergwujiaxin@163.com
Abstract:
 Crude glycerol is a major byproduct of biodiesel manufacture, and can be fractionally distilled. We present here the microbial conversion of crude glycerol into 1, 3-propanediol by Klebsiella pneumoniae under micro-aerobic conditions. We evaluated the effects of pure and crude glycerol on microbial growth and resulting product through batch culture fermentation. In addition, we studied effect of fatty acid and methyl ester on fermentation. Comparison of final concentrations and 1, 3-propanediol molar yields indicate that the crude glycerol from biodiesel production can be used as cheap raw material in the production of 1, 3-propanediol by Klebsiella pneumoniae. Using pure glycerol, concentration reaches 81.10 g/L and yield is 0.62 mol/mol, while crude glycerol from biodiesel production yields 76.85g/L concentration and 0.57mol/mol yield. Fatty acid shows itself favorable for 1, 3-propanediol production, while methyl ester causes strong inhibition of 1,3-propanediol production.
Start & End Page 
 718 - 725
Received Date 
 2014-12-18
Revised Date 
Accepted Date 
 2015-04-30
Full Text 
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Keyword 
 1, 3-propanediol, Klebsiella pneumoniae, biodiesel, methyl ester, fatty acid
Volume 
 Vol.43 No.4 (JULY 2016)
DOI 
Citation 
 Wu*[a] J., Wang[b] F., Wang[b] Z., Ye[c] H. and Liu[d] P., 1, 3-Propanediol Production from Crude Glycerol by Klebsiella pneumoniae , Chiang Mai Journal of Science, 2016; 43(4): 718-725.
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