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Assessment of genetic diversity of Jatropha curcas L. using AFLP and ISSR markers


Paper Type 
 Contributed Paper
Title 
 Assessment of genetic diversity of Jatropha curcas L. using AFLP and ISSR markers
Author 
 Sirisak Soonthornyatara, Prapa Sripichitt, Rugsarit Kaveeta and Vipa Hongtrakul
Email 
 fscivph@ku.ac.th
Abstract:

 Jatropha curcas L. (family Euphobiaceae) is widely distributed in the tropics as a potential biofuel crop. Two DNA marker techniques, AFLP and ISSR, were used to assess the genetic diversity of J. curcas germplasm collected throughout Thailand, introduced accessions from six countries and other four Jatropha species. The AFLP and ISSR fingerprint data were combined and used to evaluate genetic relationships among all samples. Pairwise similarity estimated between J. curcas ranged from 0.8428 to 1.00 with an average of 0.9955. The UPGMA cluster analysis result showed that almost all J. curcas samples were grouped into cluster 1, while a sample from USA formed separate cluster 2. The germplasms from other countries, preferably from the center of origin of this species, should be introduced to broaden genetic diversity of J. curcas in Thailand. The genetic relationship among J. curcas, J. integerrima, J. gossypifolia, J. multifida and J. podagrica was also observed. The result showed that J. integerrima was genetically more close to J. curcas than the others, while the highest value of genetic distance was found between J. curcas and J. gossypifolia. This genetic information will be useful for germplasm management in Jatropha breeding program.
Start & End Page 
 614 - 625
Received Date 
 2014-08-01
Revised Date 
Accepted Date 
 2015-04-20
Full Text 
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Keyword 
 genetic diversity, Jatropha curcas L., DNA marker, biodiesel, biofuel plant
Volume 
 Vol.42 No.3 (JULY 2015)
DOI 
Citation 
 Soonthornyatara S., Sripichitt P., Kaveeta R. and Hongtrakul V., Assessment of genetic diversity of Jatropha curcas L. using AFLP and ISSR markers, Chiang Mai Journal of Science, 2015; 42(3): 614-625.
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