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Optimization of Culture Conditions for Oil Production by the Double Mutant of Chlorella sorokiniana DMKU5202-D223


Paper Type 
 Contributed Paper
Title 
 Optimization of Culture Conditions for Oil Production by the Double Mutant of Chlorella sorokiniana DMKU5202-D223
Author 
 Mathurin Vichachucherd, Wichien Yongmanitchai and Duenrut Chonudomkul *
Email 
 fscidrc@ku.ac.th
Abstract:
 Chlorella sp. is a green microalga and well-known for its ability to accumulate high
amount of lipid in the cells. This alga was considered to be a potential energy source for
biodiesel production. The objectives of this study were to re-mutate the Chlorella sorokiniana
DMKU5202-31 which was the UV mutant strain by using Ethyl methanesulfonate (EMS), a
chemical mutagen and optimization of culture condition of the double mutant strain for oil
production. After treatment with 0.5 M EMS for 1 h 40,323 colonies was obtained and subjected
to screening. The double mutant strain C. sorokiniana DMKU5202-D223 was selected due to
its high lipid production. For optimization of culture condition, the Central Composite Design
(CCD) and Response surface methodology (RSM) were employed. The experiment model
concluded that glucose concentration of 10 g/L, pH 8, 3% CO2 and light intensity 3,000 lux
were suitable to enhance oil production. The optimization of oil production conditions of
C. sorokiniana DMKU5202-D223 showed from the RSM and CCD as followed: 6.32 g/L of
biomass production, 0.65 g/L of lipid production, and 10.45% of lipid content within 5 days.
Comparison of oil production by the double mutant strain under these optimum conditions
to the UV mutant strain and the wild type strain revealed that the double mutant strain had
oil production higher than UV mutant strain and wild type strain. The UV mutant strain and
wild type strain had lipid production 0.54 g/L and 0.22 g/L while lipid content were 8.38%
and 4.96%. In conclusion, the double mutant strain showed relatively high oil production and
could be used as the raw material for biodiesel production.
Start & End Page 
 1055 - 1068
Received Date 
 2019-04-10
Revised Date 
Accepted Date 
 2019-07-04
Full Text 
   Download
Keyword 
 biodiesel, Chlorella, mutation, optimization, microalgae
Volume 
 Vol.46 No.6 (November 2019)
DOI 
Citation 
 Vichachucherd M., Yongmanitchai W. and Chonudomkul D., Optimization of Culture Conditions for Oil Production by the Double Mutant of Chlorella sorokiniana DMKU5202-D223, Chiang Mai Journal of Science, 2019; 46(6): 1055-1068.
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