Optimization of Culture Conditions for Oil Production by the Double Mutant of Chlorella sorokiniana DMKU5202-D223
Mathurin Vichachucherd, Wichien Yongmanitchai and Duenrut Chonudomkul ** Author for corresponding; e-mail address: fscidrc@ku.ac.th
Volume: Vol.46 No.6 (November 2019)
Research Article
DOI:
Received: 10 April 2019, Revised: -, Accepted: 4 July 2019, Published: -
Citation: Vichachucherd M., Yongmanitchai W. and Chonudomkul D., Optimization of Culture Conditions for Oil Production by the Double Mutant of Chlorella sorokiniana DMKU5202-D223, Chiang Mai Journal of Science, 2019; 46(6): 1055-1068.
Abstract
Chlorella sp. is a green microalga and well-known for its ability to accumulate high amount of lipid in the cells. This alga was considered to be a potential energy source for biodiesel production. The objectives of this study were to re-mutate the Chlorella sorokiniana DMKU5202-31 which was the UV mutant strain by using Ethyl methanesulfonate (EMS), a chemical mutagen and optimization of culture condition of the double mutant strain for oil production. After treatment with 0.5 M EMS for 1 h 40,323 colonies was obtained and subjected to screening. The double mutant strain C. sorokiniana DMKU5202-D223 was selected due to its high lipid production. For optimization of culture condition, the Central Composite Design (CCD) and Response surface methodology (RSM) were employed. The experiment model concluded that glucose concentration of 10 g/L, pH 8, 3% CO2 and light intensity 3,000 lux were suitable to enhance oil production. The optimization of oil production conditions of C. sorokiniana DMKU5202-D223 showed from the RSM and CCD as followed: 6.32 g/L of biomass production, 0.65 g/L of lipid production, and 10.45% of lipid content within 5 days. Comparison of oil production by the double mutant strain under these optimum conditions to the UV mutant strain and the wild type strain revealed that the double mutant strain had oil production higher than UV mutant strain and wild type strain. The UV mutant strain and wild type strain had lipid production 0.54 g/L and 0.22 g/L while lipid content were 8.38% and 4.96%. In conclusion, the double mutant strain showed relatively high oil production and could be used as the raw material for biodiesel production.
Related Articles
DOI: 10.12982/CMJS.2025.076.
Le Anh Pham, Thi Oanh Doan, Lan Phuong Doan, Thi My Nguyen, Thi Anh Nguyet Nguyen, Phuong Thao Pham, Thi Quynh Hoang, Thi Phuong Quynh Le, Anh Thao Nguyen, Thi Thu Lien Nguyen, Anh Tu Tran and Thi Thuy Duong
Vol.52 No.5 (September 2025)Research Article View: 547 Download: 136
DOI: 10.12982/CMJS.2025.032.
Zhang Lu and Xue Guijun
Vol.52 No.3 (May 2025)Research Article View: 771 Download: 282
DOI: 10.12982/CMJS.2025.017.
Qing Hu and Fenhua Zhu
Vol.52 No.2 (March 2025)Research Article View: 860 Download: 288
DOI: 10.12982/CMJS.2024.065.
Ramesh Basnet, Anping Du, Li Tan, Ling Guo, Yanglin Jin, Zhuolin Yi, Tianfang Huang, Yang Fang and Hai Zhao
Vol.51 No.4 (July 2024)Research Article View: 1,093 Download: 615
DOI: 10.12982/CMJS.2024.046.
Jutaporn Chanathaworn and Sommai Saramath
Vol.51 No.3 (May 2024)Research Article View: 856 Download: 586
DOI: 10.12982/CMJS.2024.027.
Jiraporn Paoaiang, Prukraya Pongyeela, Nirana Chairerk and Juntima Chungsiriporn
Vol.51 No.2 (March 2024)Research Article View: 1,510 Download: 1,722
DOI: 10.12982/CMJS.2024.003.
Jeyakrishnan Sundararaj, Movva Naga Swapna Sri, Anusha Peyyala and Vijayakumar Sivasundar
Vol.51 No.1 (January 2024)Research Article View: 1,588 Download: 2,156
DOI: 10.12982/CMJS.2023.070.
Nur Afiqah Ali and Nozieana Khairuddin
Vol.50 No.6 (November 2023)Research Article View: 1,119 Download: 1,147
DOI: 10.12982/CMJS.2023.002.
Haiyin Guo, Yuqin Ma, Wei Xu, Yatao Zhao, Zedu Yang, Yi Xu, Fei Li and Yatao Li
Vol.50 No.1 (January 2023)Research Article View: 1,440 Download: 582
DOI: 10.12982/CMJS.2022.083.
Wilasinee Kingkam, Kewalee Nilgumhang, Sasikarn Nuchdang, Bheechanat Duangdee, Vichai Puripunyavanich and Dussadee Rattanaphra
Vol.49 No.5 (September 2022)Research Article View: 1,404 Download: 836
DOI: 10.12982/CMJS.2022.077.
Ekkaphon Jaiyen, Komgrit Leksakul and Nivit Charoenchai
Vol.49 No.4 (July 2022)Research Article View: 1,412 Download: 903
DOI: 10.12982/CMJS.2022.050.
Neelamanie Yapa, Dinuka Lakmali, Krishantha S. De Zoysa, Shalika Silva, Chaminda Manawadu, Bimal M. Herath, Asanka Madhushan, Geethanjana Perera, Oshani Ratnayakae, Ranganathan Kapilan, Ajith Rathnayake, Anura I. Sirisena, Suhail Asad Samantha C. Karunarathna and Chaturanga Bamunuarachchige
Vol.49 No.3 (Special Issue II : May 2022)Review Article View: 2,159 Download: 1,374
DOI: 10.12982/CMJS.2022.006.
Narisara Jaikaew, Rafael Auras and Pakorn Opaprakasit
Vol.49 No.1 (Special Issue I : Jan 2022)Research Article View: 1,906 Download: 838
page: 580 - 599
Jakkrapong Jitjamnong*, Natthida Numwong, Narinphop Chuaykarn, Chatrawee Direksilp, Apanee Luengnaruemitchai, Napaphat Kongrit and Nonlapan Khantikulanon
Vol.48 No.2 (March 2021)Research Article View: 1,230 Download: 194
page: 607 - 613
Ukrit Jitropas, Chumphol Yunphuttha, Sumeth Siriroj and Worawat Meevasana*
Vol.47 No.4 (Special Issue II : July 2020)Research Article View: 2,235 Download: 1,232
page: 484 - 498
Jakkrapong Jitjamnong, Chachchaya Thunyaratchatanon, Apanee Luengnaruemitchai, Napaphat Kongrit, Naparat Kasetsomboon, Narinphop Chuaykarn and Nonlapan Khantikulanon
Vol.47 No.3 (May 2020)Research Article View: 1,122 Download: 744
page: 987 - 1000
Jakkrapong Jitjamnong*, Apanee Luengnaruemitchai, Napaphat Samanwonga and Narinphop Chuaykarn
Vol.46 No.5 (September 2019)Research Article View: 1,050 Download: 456
page: 822 - 838
Kubra Eryasar-Orer and Seda Karasu-Yalcin*
Vol.46 No.5 (September 2019)Research Article View: 1,055 Download: 347
page: 946 - 959
Warintorn Ruksiriwanich*, Chiranan Khantham, Pichchapa Linsaenkart, Pensak Jantrawut, Sureewan Rajchasom
Vol.46 No.5 (September 2019)Research Article View: 952 Download: 332
page: 714 - 726
Benyong Han, Fang Yin*, Shiqing Liu, Xingling Zhao, Jing Liu, Changmei Wang, Hong Yang, Wudi Zhang*
Vol.46 No.4 (July 2019)Research Article View: 1,047 Download: 636
page: 609 - 617
Vichai Puripunyavanich, Anan Piriyaphattarakit, Nuttapong Chanchula and Thunya Taychasinpitak
Vol.46 No.3 (May 2019)Research Article View: 1,071 Download: 526
page: 219 - 235
Lili Liu*, Yuanyuan Meng, Xiaoning Dai and Ke Chen
Vol.46 No.2 (March 2019)Research Article View: 1,023 Download: 327
page: 1 - 14
Sureewan Sittijunda and Alissara Reungsang
Vol.46 No.1 (January 2019)Research Article View: 1,027 Download: 416
page: 2637 - 2651
Theera Thurakit, Chayakorn Pumas, Wasu Pathom-aree, Jeeraporn Pekkoh and Yuwadee Peerapornpisal
Vol.45 NO.7 (November 2018)Research Article View: 1,603 Download: 403
page: 2597 - 2608
Pornnipa Botthong, Tanatorn Pornsawatchai, Wanticha Savedboworn, Sriwiang Tipkanon, Atipan Siammai and Wiboon Riansa-ngawong
Vol.45 NO.7 (November 2018)Research Article View: 1,221 Download: 288
page: 2438 - 2453
Thanaphorn Rakkan, Nisa Paichid, Tewan Yunu, Sappasith Klomklao and Kanokphorn Sangkharak
Vol.45 No.6 (September 2018)Research Article View: 1,552 Download: 435
page: 1888 - 1900
Wuttichai Roschat, Mattana Kacha, Boonyawan Yoosuk, Preecha Moonsin, Taweesak Sudyoadsuk and Vinich Promarak
Vol.45 No.4 (July 2018)Research Article View: 1,063 Download: 292
page: 1578 - 1594
Nay Myo Sandar
Vol.45 No.3 (May 2018)Research Article View: 1,039 Download: 726
page: 1507 - 1518
Tahir Mehmood*, Sadia Fareed, Mudassir Iqbal, Adeela Naseem and Faiza Siddique
Vol.45 No.3 (May 2018)Research Article View: 1,069 Download: 298
page: 1460 - 1470
Musa Buyukada*, Mirac Eryigit and Fatih Evrendilek
Vol.45 No.3 (May 2018)Research Article View: 1,661 Download: 290
page: 1247 - 1256
Jakkapong Thongpitak, Jeeraporn Pekkoh and Chayakorn Pumas*
Vol.45 No.3 (May 2018)Research Article View: 1,169 Download: 325
page: 1030 - 1038
Jessa Marie Millanar, Mark Daniel de Luna, Aummara Yodsa-nga and Kitirote Wantala*
Vol.45 No.2 (March 2018)Research Article View: 1,038 Download: 223
page: 680 - 691
Aeujkom Saengwong, Wichien Yongmanitchai and Duenrut Chonudomkul*
Vol.45 No.2 (March 2018)Research Article View: 1,089 Download: 361
page: 653 - 667
Sureewan Sittijunda and Alissara Reungsang*
Vol.45 No.2 (March 2018)Research Article View: 1,075 Download: 361
page: 106 - 115
Ramaraj Sathasivam, Piyawat Pongpadung, Jantana Praiboon, Anong Chirapart, Savitr Trakulnaleamsai, Sittiruk Roytrakul and Niran Juntawong
Vol.45 No.1 (January 2018)Research Article View: 1,002 Download: 382
page: 144 - 156
Pawinee Deetae*[a, e], Kwanrudee Tseng [a], Chanathinart Pasuphan [a], Atikorn Panya [b], Pawadee Methacanon [c], Waranya Temthawee [a] and Manop Suphantharika [d]
Vol.44 No.1 (JANUARY 2017)Research Article View: 2,305 Download: 413
page: 50 - 58
Wanlapa Lorliam [a], Ancharida Akaracharanya [b], Sukhumaporn Krajangsang [c], Vasana Tolieng [d] a
Vol.44 No.1 (JANUARY 2017)Research Article View: 1,103 Download: 254
page: 1016 - 1026
Sabadee Worrathampitak [a], Shinji Tokuyama [b], Vichien Kitpreechavanich [c] and Sukhumaporn Sukkhum* [a]
Vol.43 No.5 (OCTOBER 2016)Research Article View: 1,065 Download: 368
page: 718 - 725
Jiaxin Wu*[a], Fenghuan Wang[b], Zheng Wang[b], Hua Ye[c] and Peiyi Liu[d]
Vol.43 No.4 (JULY 2016)Research Article View: 951 Download: 287
page: 453 - 461
Pornpan Napaumpaiporn and Anchalee Sirikhachornkit*
Vol.43 No.3 (APRIL 2016)Research Article View: 960 Download: 751
page: 918 - 929
Tanyarat Poothawan and Khomsorn Lomthaisong
Vol.42 No.4 (OCTOBER 2015)Research Article View: 1,019 Download: 408
page: 840 - 849
Sirinda Am-aiam and Chartchai Khanongnuch
Vol.42 No.4 (OCTOBER 2015)Research Article View: 1,388 Download: 296
page: 614 - 625
Sirisak Soonthornyatara, Prapa Sripichitt, Rugsarit Kaveeta and Vipa Hongtrakul
Vol.42 No.3 (JULY 2015)Research Article View: 1,276 Download: 297
page: 549 - 559
Rameshprabu Ramaraj, David Da-Wei Tsai and Paris Honglay Chen
Vol.42 No.3 (JULY 2015)Research Article View: 949 Download: 286
page: 599 - 613
Nisa Romsomsa, Patoomporn Chim-anage, Sarote Sirisansaneeyakul
Vol.42 No.3 (JULY 2015)Research Article View: 1,382 Download: 290
page: 52 - 61
ApiradeeSiangsuepchart [a] and SaisamornLumyong*[a, b]
Vol.42 No.1 (JANUARY 2015)Research Article View: 1,031 Download: 315
page: 17 - 33
Atchara Manowattana [a], Charin Techapun [b], Phisit Seesuriyachan [b], Prasert Hanmoungjai [b] and
Vol.42 No.1 (JANUARY 2015)Research Article View: 993 Download: 239
page: 1439 - 1443
Saeed Ketabchi [a], Hossein Moosaei [b] and Ying Xiao [c]
Vol.41 No.5/2 (OCTOBER 2014)Research Article View: 913 Download: 247
page: 858 - 872
Ting-chi Wen, Cuang-rong Li, Ji-chuan Kang*,Chao Kang and Kevin D. Hyde
Vol.41 No.4 (SPECIAL ISSUE 1)Research Article View: 1,253 Download: 583
page: 547 - 555
Rameshprabu Ramaraj **[a], David D-W. Tsai [a] and Paris Honglay Chen *[a]
Vol.40 No.4 (OCTOBER 2013)Research Article View: 895 Download: 297
page: 572 - 578
Achanai Buasri, Nattawut Chaiyut and Chaiwut Nakweang
Vol.38 No.4 (OCTOBER 2011)Research Article View: 940 Download: 347
page: 270 - 279
Gurunathan Baskar [a], and Sahadevan Renganathan*[b]
Vol.38 No.2 (APRIL 2011)Research Article View: 954 Download: 231
page: 231 - 241
Mohd H.I. Ibrahim*[a,b], Norhamidi Muhamad [b], Abu B. Sulong [b], Khairur R. Jamaludin [c], Nor H.
Vol.38 No.2 (APRIL 2011)Research Article View: 972 Download: 250
page: 123 - 135
Parvinder S. Sandhu*[a], Shalini Chhabra [b]
Vol.38 (SPECIAL ISSUE 2011)Research Article View: 992 Download: 217
page: 201 - 207
Manut Jaimasith and Satit Phiyanalinmat
Vol.34 No.2 (MAY 2007)Research Article View: 990 Download: 287
page: 23 - 28
Duangkamol Yujaroen, Artiwan Shotipruk, Motonobu Goto and Mitsuru Sasaki
Vol.35 No.1 (JANUARY 2008)Research Article View: 1,034 Download: 292
page: 71 - 81
Buabarn Poonperm, Waret Veerasai* and Prapin Wilairat
Vol.27 No.2 (DECEMBER 2000)Opinion View: 946 Download: 250