e-Journal Chiang Mai Journal of Science, Faculty of Science, Chiang Mai University
 







1.0
2023CiteScore
 
33rd percentile
Powered by  Scopus

SCImago Journal & Country Rank












Journal Volumes


Submit your paper
Download Template

  JOURNAL DETAIL



Optimization of Culture Conditions for Oil Production by the Double Mutant of Chlorella sorokiniana DMKU5202-D223


Paper Type 
 Contributed Paper
Title 
 Optimization of Culture Conditions for Oil Production by the Double Mutant of Chlorella sorokiniana DMKU5202-D223
Author 
 Mathurin Vichachucherd, Wichien Yongmanitchai and Duenrut Chonudomkul *
Email 
 fscidrc@ku.ac.th
Abstract:
 Chlorella sp. is a green microalga and well-known for its ability to accumulate high
amount of lipid in the cells. This alga was considered to be a potential energy source for
biodiesel production. The objectives of this study were to re-mutate the Chlorella sorokiniana
DMKU5202-31 which was the UV mutant strain by using Ethyl methanesulfonate (EMS), a
chemical mutagen and optimization of culture condition of the double mutant strain for oil
production. After treatment with 0.5 M EMS for 1 h 40,323 colonies was obtained and subjected
to screening. The double mutant strain C. sorokiniana DMKU5202-D223 was selected due to
its high lipid production. For optimization of culture condition, the Central Composite Design
(CCD) and Response surface methodology (RSM) were employed. The experiment model
concluded that glucose concentration of 10 g/L, pH 8, 3% CO2 and light intensity 3,000 lux
were suitable to enhance oil production. The optimization of oil production conditions of
C. sorokiniana DMKU5202-D223 showed from the RSM and CCD as followed: 6.32 g/L of
biomass production, 0.65 g/L of lipid production, and 10.45% of lipid content within 5 days.
Comparison of oil production by the double mutant strain under these optimum conditions
to the UV mutant strain and the wild type strain revealed that the double mutant strain had
oil production higher than UV mutant strain and wild type strain. The UV mutant strain and
wild type strain had lipid production 0.54 g/L and 0.22 g/L while lipid content were 8.38%
and 4.96%. In conclusion, the double mutant strain showed relatively high oil production and
could be used as the raw material for biodiesel production.
Start & End Page 
 1055 - 1068
Received Date 
 2019-04-10
Revised Date 
Accepted Date 
 2019-07-04
Full Text 
   Download
Keyword 
 biodiesel, Chlorella, mutation, optimization, microalgae
Volume 
 Vol.46 No.6 (November 2019)
DOI 
Citation 
 Vichachucherd M., Yongmanitchai W. and * D.C., Optimization of Culture Conditions for Oil Production by the Double Mutant of Chlorella sorokiniana DMKU5202-D223, Chiang Mai Journal of Science, 2019; 46(6): 1055-1068.
SDGs
View:1,173 Download:425

  RELATED ARTICLE

Research on Prediction of the Digital Economy Index Based on Improved Sparrow Search Algorithm
Article ID: e2025017
Author:Qing Hu and Fenhua Zhu
Vol.52 No.2 (March 2025) View: 200 Download:64
Investigation on Selection, Improvement of Biomass Production and Protein Content in Duckweeds (Lemna Strains)
Article ID: e2024065
Author:Ramesh Basnet, Anping Du, Li Tan, Ling Guo, Yanglin Jin, Zhuolin Yi, Tianfang Huang, Yang Fang and Hai Zhao
Vol.51 No.4 (July 2024) View: 532 Download:426
Optimizing Process Parameters through Response Surface Methodology for Transesterification Using an Innovative Extruded Catalyst
Article ID: e2024046
Author:Jutaporn Chanathaworn and Sommai Saramath
Vol.51 No.3 (May 2024) View: 448 Download:282
H2S Removal from Biogas using Steel Wool (Fe2O3) Adsorption Combined with Water Absorption: Experimental and Modelling via RSM
Article ID: e2024027
Author:Jiraporn Paoaiang, Prukraya Pongyeela, Nirana Chairerk and Juntima Chungsiriporn
Vol.51 No.2 (March 2024) View: 1,080 Download:756
Grey-Taguchi Approach for Optimizing FSW Parameters in Joining AA6262 and AA5083 Alloys
Article ID: e2024003
Author:Jeyakrishnan Sundararaj, Movva Naga Swapna Sri, Anusha Peyyala and Vijayakumar Sivasundar
Vol.51 No.1 (January 2024) View: 1,108 Download:1,762
Biodiesel Production from Waste Cooking Oil using Heterogeneous CaO/Zn Catalyst: Yield and Reusability Performance
Article ID: e2023070
Author:Nur Afiqah Ali and Nozieana Khairuddin
Vol.50 No.6 (November 2023) View: 599 Download:594
Prediction of Leakage Rate and Optimization of Structural Parameter of Blade Tip Labyrinth Seal
Article ID: e2023002
Author:Haiyin Guo, Yuqin Ma, Wei Xu, Yatao Zhao, Zedu Yang, Yi Xu, Fei Li and Yatao Li
Vol.50 No.1 (January 2023) View: 1,020 Download:370
Study the Stable Diatomite-supported Catalyst for Biodiesel Production
page: 1332 - 1343
Author:Wilasinee Kingkam, Kewalee Nilgumhang, Sasikarn Nuchdang, Bheechanat Duangdee, Vichai Puripunyavanich and Dussadee Rattanaphra
Vol.49 No.5 (September 2022) View: 1,072 Download:486
Local Search Element Decomposition Method to Solve Integer and m-Integer Problems
page: 1252 - 1272
Author:Ekkaphon Jaiyen, Komgrit Leksakul and Nivit Charoenchai
Vol.49 No.4 (July 2022) View: 1,087 Download:795
SDGs:
Biofertilizers: An Emerging Trend in Agricultural Sustainability
page: 608 - 640
Author:Neelamanie Yapa, Dinuka Lakmali, Krishantha S. De Zoysa, Shalika Silva, Chaminda Manawadu, Bimal M. Herath, Asanka Madhushan, Geethanjana Perera, Oshani Ratnayakae, Ranganathan Kapilan, Ajith Rathnayake, Anura I. Sirisena, Suhail Asad Samantha C. Karunarathna and Chaturanga Bamunuarachchige
Vol.49 No.3 (Special Issue II : May 2022) View: 1,652 Download:876
Optimization of Melt-mixing Transesterification of Polylactide by Polyethylene Glycol Employing Response Surface Methodology
page: 69 - 80
Author:Narisara Jaikaew, Rafael Auras and Pakorn Opaprakasit
Vol.49 No.1 (Special Issue I : Jan 2022) View: 1,291 Download:639
Conversion of Palm Oil into Biodiesel Production with Heterogeneous Catalyst Derived from Spent Coffee Grounds Ash: Process Optimization Through Response Surface Methodology
page: 580 - 599
Author:Jakkrapong Jitjamnong*, Natthida Numwong, Narinphop Chuaykarn, Chatrawee Direksilp, Apanee Luengnaruemitchai, Napaphat Kongrit and Nonlapan Khantikulanon
Vol.48 No.2 (March 2021) View: 822 Download:193
Capacitance Optimization of Planar Carbon-based Supercapacitor
page: 607 - 613
Author:Ukrit Jitropas, Chumphol Yunphuttha, Sumeth Siriroj and Worawat Meevasana*
Vol.47 No.4 (Special Issue II : July 2020) View: 1,918 Download:1,122
Effect of Calcination Temperature of Musa sapientum Linn Peels as a Novel Biobased Heterogeneous Catalyst for Soybean Oil-derived Fatty Acid Methyl Ester Formation
page: 484 - 498
Author:Jakkrapong Jitjamnong, Chachchaya Thunyaratchatanon, Apanee Luengnaruemitchai, Napaphat Kongrit, Naparat Kasetsomboon, Narinphop Chuaykarn and Nonlapan Khantikulanon
Vol.47 No.3 (May 2020) View: 807 Download:605
Biodiesel Production from Canola Oil and Methanol Using Ba Impregnated Calcium Oxide with Microwave Irradiation-Assistance
page: 987 - 1000
Author:Jakkrapong Jitjamnong*, Apanee Luengnaruemitchai, Napaphat Samanwonga and Narinphop Chuaykarn
Vol.46 No.5 (September 2019) View: 733 Download:342
Bioproduction of Xylitol From Chestnut Shell Hydrolysate: Optimization of Fermentation Parameters
page: 822 - 838
Author:Kubra Eryasar-Orer and Seda Karasu-Yalcin*
Vol.46 No.5 (September 2019) View: 793 Download:288
Optimization of Placenta Extraction for Wound Healing Activity
page: 946 - 959
Author:Warintorn Ruksiriwanich*, Chiranan Khantham, Pichchapa Linsaenkart, Pensak Jantrawut, Sureewan Rajchasom
Vol.46 No.5 (September 2019) View: 651 Download:274
Optimization of Oleic Acid Esterification for Biodiesel Production using Brønsted Acidic Ionic Liquid as a Catalyst
page: 714 - 726
Author:Benyong Han, Fang Yin*, Shiqing Liu, Xingling Zhao, Jing Liu, Changmei Wang, Hong Yang, Wudi Zhang*
Vol.46 No.4 (July 2019) View: 749 Download:389
Mutation Induction of In Vitro Chrysanthemum by Gamma Irradiation
page: 609 - 617
Author:Vichai Puripunyavanich, Anan Piriyaphattarakit, Nuttapong Chanchula and Thunya Taychasinpitak
Vol.46 No.3 (May 2019) View: 751 Download:440
Isolation of A Novel Collagenase-producing Strain from Animal Bone Wastes and Optimization of Its Enzyme Production
page: 219 - 235
Author:Lili Liu*, Yuanyuan Meng, Xiaoning Dai and Ke Chen
Vol.46 No.2 (March 2019) View: 737 Download:267
Bioconversion of Crude Glycerol into 1,3-propanediol by an Anaerobic Mixed Microbial Consortium
page: 1 - 14
Author:Sureewan Sittijunda and Alissara Reungsang
Vol.46 No.1 (January 2019) View: 719 Download:256
Enhancement of Biomass, Lipid and Hydrocarbon Production from Green Microalga, Botryococcus braunii AARL G037, by UV-C Induction
page: 2637 - 2651
Author:Theera Thurakit, Chayakorn Pumas, Wasu Pathom-aree, Jeeraporn Pekkoh and Yuwadee Peerapornpisal
Vol.45 NO.7 (November 2018) View: 1,282 Download:303
Optimization and Performance of Covered-Light Structures for Hydrogen Production by Rhodopseudomonas palustris TN1
page: 2597 - 2608
Author:Pornnipa Botthong, Tanatorn Pornsawatchai, Wanticha Savedboworn, Sriwiang Tipkanon, Atipan Siammai and Wiboon Riansa-ngawong
Vol.45 NO.7 (November 2018) View: 930 Download:245
Synthesis and Characterization of Biolubricant from POME Oil and Hepatopancreas Lipase from Pacific White Shrimp (Litopenaeus vannamei)
page: 2438 - 2453
Author:Thanaphorn Rakkan, Nisa Paichid, Tewan Yunu, Sappasith Klomklao and Kanokphorn Sangkharak
Vol.45 No.6 (September 2018) View: 1,214 Download:361
Efficient Conversion of Oils to Biodiesel Catalyzed by Uncalcined Disodium Metasilicate Granules
page: 1888 - 1900
Author:Wuttichai Roschat, Mattana Kacha, Boonyawan Yoosuk, Preecha Moonsin, Taweesak Sudyoadsuk and Vinich Promarak
Vol.45 No.4 (July 2018) View: 798 Download:227
Capacity Planning of Aggregators, Provisioning of Software Defined Network and Cloud Resources to Applications of Wireless Sensor Networks
page: 1578 - 1594
Author:Nay Myo Sandar
Vol.45 No.3 (May 2018) View: 766 Download:655
Utilization of Agro-waste and Non-conventional Eruca sativa Seed Oil for Getting Optimized Process to Acquire Better Yield of Biodiesel by Using Response Surface Methodology (RSM)
page: 1507 - 1518
Author:Tahir Mehmood*, Sadia Fareed, Mudassir Iqbal, Adeela Naseem and Faiza Siddique
Vol.45 No.3 (May 2018) View: 728 Download:229
Concurrent Optimization of Decolorization, COD Removal, and Their Costs in Response to Photocatalytic Degradation Integrating Experimental Designs, Artificial Immune System, and Empirical Modeling
page: 1460 - 1470
Author:Musa Buyukada*, Mirac Eryigit and Fatih Evrendilek
Vol.45 No.3 (May 2018) View: 1,326 Download:250
Simple Medium Formulation for Manganese Remediation by Green Microalga Pediastrum duplex AARLG060
page: 1247 - 1256
Author:Jakkapong Thongpitak, Jeeraporn Pekkoh and Chayakorn Pumas*
Vol.45 No.3 (May 2018) View: 888 Download:273
Toluene Oxidation using K-OMS 2 Synthesized Via Hydrothermal Process by Central Composite Design
page: 1030 - 1038
Author:Jessa Marie Millanar, Mark Daniel de Luna, Aummara Yodsa-nga and Kitirote Wantala*
Vol.45 No.2 (March 2018) View: 738 Download:177
Screening and Optimization of Squalene Production from Microalgae Aurantiochytrium sp.
page: 680 - 691
Author:Aeujkom Saengwong, Wichien Yongmanitchai and Duenrut Chonudomkul*
Vol.45 No.2 (March 2018) View: 750 Download:297
Biohydrogen Production from Crude Glycerol Using Anaerobic Mixed Cultures: Media Compositions Optimization
page: 653 - 667
Author:Sureewan Sittijunda and Alissara Reungsang*
Vol.45 No.2 (March 2018) View: 806 Download:274
Optimizing NaCl and KNO3 Concentrations for High b-carotene Production in Photobioreactor by Dunaliella salina KU11 Isolated from Saline Soil Sample
page: 106 - 115
Author:Ramaraj Sathasivam, Piyawat Pongpadung, Jantana Praiboon, Anong Chirapart, Savitr Trakulnaleamsai, Sittiruk Roytrakul and Niran Juntawong
Vol.45 No.1 (January 2018) View: 736 Download:302
Ultrasonic Extraction, Antioxidant and Anti-glycation Activities of Polysaccharides from Gynura divaricata Leaves
page: 144 - 156
Author:Pawinee Deetae*[a, e], Kwanrudee Tseng [a], Chanathinart Pasuphan [a], Atikorn Panya [b], Pawadee Methacanon [c], Waranya Temthawee [a] and Manop Suphantharika [d]
Vol.44 No.1 (JANUARY 2017) View: 1,756 Download:320
Optimization of Xylitol Production by Candida tropicalis A26
page: 50 - 58
Author:Wanlapa Lorliam [a], Ancharida Akaracharanya [b], Sukhumaporn Krajangsang [c], Vasana Tolieng [d] a
Vol.44 No.1 (JANUARY 2017) View: 793 Download:212
Utilization of Non-rubber Skim Latex for Poly(L-lactide)-degrading Enzyme Production by Actinomadura keratinilytica Strain T16-1
page: 1016 - 1026
Author:Sabadee Worrathampitak [a], Shinji Tokuyama [b], Vichien Kitpreechavanich [c] and Sukhumaporn Sukkhum* [a]
Vol.43 No.5 (OCTOBER 2016) View: 741 Download:305
1, 3-Propanediol Production from Crude Glycerol by Klebsiella pneumoniae
page: 718 - 725
Author:Jiaxin Wu*[a], Fenghuan Wang[b], Zheng Wang[b], Hua Ye[c] and Peiyi Liu[d]
Vol.43 No.4 (JULY 2016) View: 677 Download:236
Effects of high temperature on Carotenoid Accumulation and Gene Expression in the Model Green Alga Chlamydomonas reinhardtii
page: 453 - 461
Author:Pornpan Napaumpaiporn and Anchalee Sirikhachornkit*
Vol.43 No.3 (APRIL 2016) View: 704 Download:534
Analysis of Chitin, Chitosan, and Optimization for Carotenoids Extraction Yield with Rice Bran Oil from Thai Fairy Shrimp
page: 918 - 929
Author:Tanyarat Poothawan and Khomsorn Lomthaisong
Vol.42 No.4 (OCTOBER 2015) View: 700 Download:222
Medium Optimization for b-Galactosidase Production by a Thermotolerant Yeast
page: 840 - 849
Author:Sirinda Am-aiam and Chartchai Khanongnuch
Vol.42 No.4 (OCTOBER 2015) View: 1,141 Download:243
Assessment of genetic diversity of Jatropha curcas L. using AFLP and ISSR markers
page: 614 - 625
Author:Sirisak Soonthornyatara, Prapa Sripichitt, Rugsarit Kaveeta and Vipa Hongtrakul
Vol.42 No.3 (JULY 2015) View: 1,045 Download:230
Detention Time Study of Algal Biomass Production with Natural Water Medium
page: 549 - 559
Author:Rameshprabu Ramaraj, David Da-Wei Tsai and Paris Honglay Chen
Vol.42 No.3 (JULY 2015) View: 665 Download:228
Improvement of production and stability of silk degumming protease by Bacillus sp. C4 SS-2013
page: 599 - 613
Author:Nisa Romsomsa, Patoomporn Chim-anage, Sarote Sirisansaneeyakul
Vol.42 No.3 (JULY 2015) View: 1,080 Download:218
Statistical Optimization for the Production of D-Mannose Isomerase by Saccharothrix sp. CMU-K747 Using Response Surface Methodology
page: 52 - 61
Author:ApiradeeSiangsuepchart [a] and SaisamornLumyong*[a, b]
Vol.42 No.1 (JANUARY 2015) View: 711 Download:251
β-Carotene Production by Sporobolomyces pararoseus TISTR5213 Using Crude Glycerol as the Sole Carbon Source
page: 17 - 33
Author:Atchara Manowattana [a], Charin Techapun [b], Phisit Seesuriyachan [b], Prasert Hanmoungjai [b] and
Vol.42 No.1 (JANUARY 2015) View: 676 Download:181
Minimal Changes in the Right Hand Side Data for Correcting Linear Infeasibility Arising in Intensity-Modulated Radiation Therapy with the Generalized Newton Method
page: 1439 - 1443
Author:Saeed Ketabchi [a], Hossein Moosaei [b] and Ying Xiao [c]
Vol.41 No.5/2 (OCTOBER 2014) View: 702 Download:195
Optimization of Solid-state Fermentation for Fruiting Body Growth and Cordecepin Production by Cordyceps militaris
page: 858 - 872
Author:Ting-chi Wen, Cuang-rong Li, Ji-chuan Kang*,Chao Kang and Kevin D. Hyde
Vol.41 No.4 (SPECIAL ISSUE 1) View: 817 Download:531
Chlorophyll is not accurate measurement for algal biomass
page: 547 - 555
Author:Rameshprabu Ramaraj **[a], David D-W. Tsai [a] and Paris Honglay Chen *[a]
Vol.40 No.4 (OCTOBER 2013) View: 671 Download:255
Preparing Activated Carbon from Palm Shell for Biodiesel Fuel Production
page: 572 - 578
Author:Achanai Buasri, Nattawut Chaiyut and Chaiwut Nakweang
Vol.38 No.4 (OCTOBER 2011) View: 654 Download:305
Optimization of Media Components and Operating Conditions for Exogenous Production of Fungal L-asparaginase
page: 270 - 279
Author:Gurunathan Baskar [a], and Sahadevan Renganathan*[b]
Vol.38 No.2 (APRIL 2011) View: 698 Download:160
Optimization of Micro Metal Injection Molding with Multiple Performance Characteristics using Grey Relational Grade
page: 231 - 241
Author:Mohd H.I. Ibrahim*[a,b], Norhamidi Muhamad [b], Abu B. Sulong [b], Khairur R. Jamaludin [c], Nor H.
Vol.38 No.2 (APRIL 2011) View: 676 Download:195
A Comparative Analysis of Conjugate Gradient Algorithms & PSO Based Neural Network Approaches for Reusability Evaluation of Procedure Based Software Systems
page: 123 - 135
Author:Parvinder S. Sandhu*[a], Shalini Chhabra [b]
Vol.38 (SPECIAL ISSUE 2011) View: 725 Download:190
Biodiesel Synthesis from Transesterification by Clay-based Catalyst
page: 201 - 207
Author:Manut Jaimasith and Satit Phiyanalinmat
Vol.34 No.2 (MAY 2007) View: 717 Download:218
Production Methyl Esters from Palm Fatty Acids in Supercritical Methanol
page: 23 - 28
Author:Duangkamol Yujaroen, Artiwan Shotipruk, Motonobu Goto and Mitsuru Sasaki
Vol.35 No.1 (JANUARY 2008) View: 723 Download:220
Sensitivity Enhancement of Differential Pulse Polarographic Technique by Simplex Optimization Method
page: 71 - 81
Author:Buabarn Poonperm, Waret Veerasai* and Prapin Wilairat
Vol.27 No.2 (DECEMBER 2000) View: 654 Download:184



Search in this journal


Document Search


Author Search

A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z

Popular Search






Chiang Mai Journal of Science

Faculty of Science, Chiang Mai University
239 Huaykaew Road, Tumbol Suthep, Amphur Muang, Chiang Mai 50200 THAILAND
Tel: +6653-943-467




Faculty of Science,
Chiang Mai University




EMAIL
cmjs@cmu.ac.th




Copyrights © Since 2021 All Rights Reserved by Chiang Mai Journal of Science