Chiang Mai Journal of Science

Statistical Optimization for the Production of D-Mannose Isomerase by Saccharothrix sp. CMU-K747 Using Response Surface Methodology

Paper Type	Contributed Paper
Title	Statistical Optimization for the Production of D-Mannose Isomerase by Saccharothrix sp. CMU-K747 Using Response Surface Methodology
Author	ApiradeeSiangsuepchart [a] and SaisamornLumyong*[a, b]
Email	scboi009@chiangmai.ac.th
Abstract:  Response surface methodology (RSM) and central composite design (CCD) were employed to find the optimal fermentation medium for producing intracellular D-mannose isomerase (D-MI) using Saccharothrix sp. CMU-K747. The two variables involved in this study were D-mannose and soybean meal. The statistical analysis of the results showed that, only D-mannose had a significant effect on D-MI production. The optimized medium containing (% w/v) D-mannose, 1.3 and soybean meal, 0.055 resulted in 1.32-fold enhanced in D-MI (1.638 Unit/ml) production compared to the initial level (1.243 Unit/ml). In addition, the effects of incubation period, pH and temperature of D-MI production in the optimized medium composition were also described. This present study is the first report on statistical optimization of medium components for D-MI production and also explored the possibility of using D-MI produced by Saccharothrix sp. CMU-K747 for rare sugar production.
Start & End Page	52 - 61
Received Date	2013-10-16
Revised Date
Accepted Date	2013-12-12
Full Text	Download
Keyword	Response surface methodology, Medium optimization, D-Mannose isomerase production, Central composite design, Saccharothrixsp. CMU-K747
Volume	Vol.42 No.1 (JANUARY 2015)
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