Statistical Optimization for the Production of D-Mannose Isomerase by Saccharothrix sp. CMU-K747 Using Response Surface Methodology
ApiradeeSiangsuepchart [a] and SaisamornLumyong*[a, b]* Author for corresponding; e-mail address: scboi009@chiangmai.ac.th
Volume: Vol.42 No.1 (JANUARY 2015)
Research Article
DOI:
Received: 16 October 2013, Revised: -, Accepted: 12 December 2013, Published: -
Citation: Apiradeesiangsuepchart and B] S., Statistical Optimization for the Production of D-Mannose Isomerase by Saccharothrix sp. CMU-K747 Using Response Surface Methodology, Chiang Mai Journal of Science, 2015; 42(1): 52-61.
Abstract
Response surface methodology (RSM) and central composite design (CCD) were employed to find the optimal fermentation medium for producing intracellular D-mannose isomerase (D-MI) using Saccharothrix sp. CMU-K747. The two variables involved in this study were D-mannose and soybean meal. The statistical analysis of the results showed that, only D-mannose had a significant effect on D-MI production. The optimized medium containing (% w/v) D-mannose, 1.3 and soybean meal, 0.055 resulted in 1.32-fold enhanced in D-MI (1.638 Unit/ml) production compared to the initial level (1.243 Unit/ml). In addition, the effects of incubation period, pH and temperature of D-MI production in the optimized medium composition were also described. This present study is the first report on statistical optimization of medium components for D-MI production and also explored the possibility of using D-MI produced by Saccharothrix sp. CMU-K747 for rare sugar production.