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Statistical Optimization for the Production of D-Mannose Isomerase by Saccharothrix sp. CMU-K747 Using Response Surface Methodology
Paper Type  |
Contributed Paper |
Title |
Statistical Optimization for the Production of D-Mannose Isomerase by Saccharothrix sp. CMU-K747 Using Response Surface Methodology |
Author |
ApiradeeSiangsuepchart [a] and SaisamornLumyong*[a, b] |
Email |
scboi009@chiangmai.ac.th |
|
Abstract: Response surface methodology (RSM) and central composite design (CCD) were employed to find the optimal fermentation medium for producing intracellular D-mannose isomerase (D-MI) using Saccharothrix sp. CMU-K747. The two variables involved in this study were D-mannose and soybean meal. The statistical analysis of the results showed that, only D-mannose had a significant effect on D-MI production. The optimized medium containing (% w/v) D-mannose, 1.3 and soybean meal, 0.055 resulted in 1.32-fold enhanced in D-MI (1.638 Unit/ml) production compared to the initial level (1.243 Unit/ml). In addition, the effects of incubation period, pH and temperature of D-MI production in the optimized medium composition were also described. This present study is the first report on statistical optimization of medium components for D-MI production and also explored the possibility of using D-MI produced by Saccharothrix sp. CMU-K747 for rare sugar production.
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|
Start & End Page |
52 - 61 |
Received Date |
2013-10-16 |
Revised Date |
|
Accepted Date |
2013-12-12 |
Full Text |
Download |
Keyword |
Response surface methodology, Medium optimization, D-Mannose isomerase production, Central composite design, Saccharothrixsp. CMU-K747 |
Volume |
Vol.42 No.1 (JANUARY 2015) |
DOI |
|
Citation |
Apiradeesiangsuepchart and B] S., Statistical Optimization for the Production of D-Mannose Isomerase by Saccharothrix sp. CMU-K747 Using Response Surface Methodology, Chiang Mai J. Sci., 2015; 42(1): 52-61. |
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| View:586 Download:223 | |
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