No Session
Chiang Mai Journal of Science, Faculty of Science, Chiang Mai University
 







1.0
2023CiteScore
 
33rd percentile
Powered by  Scopus

SCImago Journal & Country Rank












Journal Volumes


Submit your paper
Download Template

  JOURNAL DETAIL



Screening and Optimization of Squalene Production from Microalgae Aurantiochytrium sp.


Paper Type 
 Contributed Paper
Title 
 Screening and Optimization of Squalene Production from Microalgae Aurantiochytrium sp.
Author 
 Aeujkom Saengwong, Wichien Yongmanitchai and Duenrut Chonudomkul*
Email 
 fscidrc@ku.ac.th
Abstract:
Microalgae are an alternative potential source of squalene because they grow rapidly, relatively easy to culture and accumulate large amounts of squalene. The objectives of this research are to isolate and screen squalene producing heterotrophic microalgae from mangrove forests of Thailand and to optimize culture conditions. Five hundred and eighty-five strains of the microalgae were isolated from the mangrove forests along the gulf of Thailand and southern, Thailand. After screening, the isolate S02-459 was selected for further studied due to its high squalene content. Sequencing of partial 18S rRNA gene revealed that it belong to the genus Aurantiochytrium. Evaluation of eight factors influencing growth and squalene accumulation, i.e., glucose concentration, yeast extract, peptone, monosodium glutamate, pH, shaking speed, salinity and temperature were carried out by using Plackett-Burman design. Four factors, namely, glucose concentration, yeast extract concentration, peptone concentration and shaking speed were significantly (p<0.05) affected biomass and squalene production. Central Composite design was employed for optimized these four parameters for enhancement the squalene production. These factors were assessed using quadratic model. The coefficient of determination (R2) of more than 0.9 and p-value less than 0.05 indicated that the model was acceptable. The optimized conditions from CCD showed that 30 g/L of glucose, 5.0 g/L of yeast extract, 20 g/L of peptone and shaking speed at 75 rpm were appropriate. Verifications of the predicted values were 41.19±1.86 mg/L of squalene production and 10.85±0.10 mg/g cell dry weight of squalene content. The deviation of experimental values and predicted values for squalene production and content were -1.81% and -6.78%, respectively.
Start & End Page 
 680 - 691
Received Date 
 2016-11-15
Revised Date 
Accepted Date 
 2017-03-24
Full Text 
   Download
Keyword 
 microalgae, Aurantiochytrium, squalene, optimization, central composite design
Volume 
 Vol.45 No.2 (March 2018)
DOI 
Citation 
 Saengwong A., Yongmanitchai W. and Chonudomkul* D., Screening and Optimization of Squalene Production from Microalgae Aurantiochytrium sp., Chiang Mai Journal of Science, 2018; 45(2): 680-691.
SDGs
View:756 Download:300

  RELATED ARTICLE

Research on Prediction of the Digital Economy Index Based on Improved Sparrow Search Algorithm
Article ID: e2025017
Author:Qing Hu and Fenhua Zhu
Vol.52 No.2 (March 2025) View: 251 Download:100
Investigation on Selection, Improvement of Biomass Production and Protein Content in Duckweeds (Lemna Strains)
Article ID: e2024065
Author:Ramesh Basnet, Anping Du, Li Tan, Ling Guo, Yanglin Jin, Zhuolin Yi, Tianfang Huang, Yang Fang and Hai Zhao
Vol.51 No.4 (July 2024) View: 543 Download:435
Optimizing Process Parameters through Response Surface Methodology for Transesterification Using an Innovative Extruded Catalyst
Article ID: e2024046
Author:Jutaporn Chanathaworn and Sommai Saramath
Vol.51 No.3 (May 2024) View: 460 Download:295
H2S Removal from Biogas using Steel Wool (Fe2O3) Adsorption Combined with Water Absorption: Experimental and Modelling via RSM
Article ID: e2024027
Author:Jiraporn Paoaiang, Prukraya Pongyeela, Nirana Chairerk and Juntima Chungsiriporn
Vol.51 No.2 (March 2024) View: 1,092 Download:807
Grey-Taguchi Approach for Optimizing FSW Parameters in Joining AA6262 and AA5083 Alloys
Article ID: e2024003
Author:Jeyakrishnan Sundararaj, Movva Naga Swapna Sri, Anusha Peyyala and Vijayakumar Sivasundar
Vol.51 No.1 (January 2024) View: 1,118 Download:1,778
Prediction of Leakage Rate and Optimization of Structural Parameter of Blade Tip Labyrinth Seal
Article ID: e2023002
Author:Haiyin Guo, Yuqin Ma, Wei Xu, Yatao Zhao, Zedu Yang, Yi Xu, Fei Li and Yatao Li
Vol.50 No.1 (January 2023) View: 1,051 Download:383
Local Search Element Decomposition Method to Solve Integer and m-Integer Problems
page: 1252 - 1272
Author:Ekkaphon Jaiyen, Komgrit Leksakul and Nivit Charoenchai
Vol.49 No.4 (July 2022) View: 1,097 Download:801
SDGs:
Biofertilizers: An Emerging Trend in Agricultural Sustainability
page: 608 - 640
Author:Neelamanie Yapa, Dinuka Lakmali, Krishantha S. De Zoysa, Shalika Silva, Chaminda Manawadu, Bimal M. Herath, Asanka Madhushan, Geethanjana Perera, Oshani Ratnayakae, Ranganathan Kapilan, Ajith Rathnayake, Anura I. Sirisena, Suhail Asad Samantha C. Karunarathna and Chaturanga Bamunuarachchige
Vol.49 No.3 (Special Issue II : May 2022) View: 1,663 Download:905
Optimization of Melt-mixing Transesterification of Polylactide by Polyethylene Glycol Employing Response Surface Methodology
page: 69 - 80
Author:Narisara Jaikaew, Rafael Auras and Pakorn Opaprakasit
Vol.49 No.1 (Special Issue I : Jan 2022) View: 1,305 Download:651
Capacitance Optimization of Planar Carbon-based Supercapacitor
page: 607 - 613
Author:Ukrit Jitropas, Chumphol Yunphuttha, Sumeth Siriroj and Worawat Meevasana*
Vol.47 No.4 (Special Issue II : July 2020) View: 1,936 Download:1,135
Optimization of Culture Conditions for Oil Production by the Double Mutant of Chlorella sorokiniana DMKU5202-D223
page: 1055 - 1068
Author:Mathurin Vichachucherd, Wichien Yongmanitchai and Duenrut Chonudomkul *
Vol.46 No.6 (November 2019) View: 1,179 Download:429
Bioproduction of Xylitol From Chestnut Shell Hydrolysate: Optimization of Fermentation Parameters
page: 822 - 838
Author:Kubra Eryasar-Orer and Seda Karasu-Yalcin*
Vol.46 No.5 (September 2019) View: 797 Download:291
Optimization of Placenta Extraction for Wound Healing Activity
page: 946 - 959
Author:Warintorn Ruksiriwanich*, Chiranan Khantham, Pichchapa Linsaenkart, Pensak Jantrawut, Sureewan Rajchasom
Vol.46 No.5 (September 2019) View: 669 Download:279
Optimization of Oleic Acid Esterification for Biodiesel Production using Brønsted Acidic Ionic Liquid as a Catalyst
page: 714 - 726
Author:Benyong Han, Fang Yin*, Shiqing Liu, Xingling Zhao, Jing Liu, Changmei Wang, Hong Yang, Wudi Zhang*
Vol.46 No.4 (July 2019) View: 754 Download:395
Isolation of A Novel Collagenase-producing Strain from Animal Bone Wastes and Optimization of Its Enzyme Production
page: 219 - 235
Author:Lili Liu*, Yuanyuan Meng, Xiaoning Dai and Ke Chen
Vol.46 No.2 (March 2019) View: 748 Download:269
Optimization and Performance of Covered-Light Structures for Hydrogen Production by Rhodopseudomonas palustris TN1
page: 2597 - 2608
Author:Pornnipa Botthong, Tanatorn Pornsawatchai, Wanticha Savedboworn, Sriwiang Tipkanon, Atipan Siammai and Wiboon Riansa-ngawong
Vol.45 NO.7 (November 2018) View: 935 Download:247
Capacity Planning of Aggregators, Provisioning of Software Defined Network and Cloud Resources to Applications of Wireless Sensor Networks
page: 1578 - 1594
Author:Nay Myo Sandar
Vol.45 No.3 (May 2018) View: 778 Download:659
Utilization of Agro-waste and Non-conventional Eruca sativa Seed Oil for Getting Optimized Process to Acquire Better Yield of Biodiesel by Using Response Surface Methodology (RSM)
page: 1507 - 1518
Author:Tahir Mehmood*, Sadia Fareed, Mudassir Iqbal, Adeela Naseem and Faiza Siddique
Vol.45 No.3 (May 2018) View: 736 Download:232
Concurrent Optimization of Decolorization, COD Removal, and Their Costs in Response to Photocatalytic Degradation Integrating Experimental Designs, Artificial Immune System, and Empirical Modeling
page: 1460 - 1470
Author:Musa Buyukada*, Mirac Eryigit and Fatih Evrendilek
Vol.45 No.3 (May 2018) View: 1,329 Download:252
Simple Medium Formulation for Manganese Remediation by Green Microalga Pediastrum duplex AARLG060
page: 1247 - 1256
Author:Jakkapong Thongpitak, Jeeraporn Pekkoh and Chayakorn Pumas*
Vol.45 No.3 (May 2018) View: 895 Download:275
Toluene Oxidation using K-OMS 2 Synthesized Via Hydrothermal Process by Central Composite Design
page: 1030 - 1038
Author:Jessa Marie Millanar, Mark Daniel de Luna, Aummara Yodsa-nga and Kitirote Wantala*
Vol.45 No.2 (March 2018) View: 743 Download:180
Biohydrogen Production from Crude Glycerol Using Anaerobic Mixed Cultures: Media Compositions Optimization
page: 653 - 667
Author:Sureewan Sittijunda and Alissara Reungsang*
Vol.45 No.2 (March 2018) View: 809 Download:284
Optimizing NaCl and KNO3 Concentrations for High b-carotene Production in Photobioreactor by Dunaliella salina KU11 Isolated from Saline Soil Sample
page: 106 - 115
Author:Ramaraj Sathasivam, Piyawat Pongpadung, Jantana Praiboon, Anong Chirapart, Savitr Trakulnaleamsai, Sittiruk Roytrakul and Niran Juntawong
Vol.45 No.1 (January 2018) View: 743 Download:305
Ultrasonic Extraction, Antioxidant and Anti-glycation Activities of Polysaccharides from Gynura divaricata Leaves
page: 144 - 156
Author:Pawinee Deetae*[a, e], Kwanrudee Tseng [a], Chanathinart Pasuphan [a], Atikorn Panya [b], Pawadee Methacanon [c], Waranya Temthawee [a] and Manop Suphantharika [d]
Vol.44 No.1 (JANUARY 2017) View: 1,762 Download:324
Optimization of Xylitol Production by Candida tropicalis A26
page: 50 - 58
Author:Wanlapa Lorliam [a], Ancharida Akaracharanya [b], Sukhumaporn Krajangsang [c], Vasana Tolieng [d] a
Vol.44 No.1 (JANUARY 2017) View: 799 Download:215
Utilization of Non-rubber Skim Latex for Poly(L-lactide)-degrading Enzyme Production by Actinomadura keratinilytica Strain T16-1
page: 1016 - 1026
Author:Sabadee Worrathampitak [a], Shinji Tokuyama [b], Vichien Kitpreechavanich [c] and Sukhumaporn Sukkhum* [a]
Vol.43 No.5 (OCTOBER 2016) View: 745 Download:315
Effects of high temperature on Carotenoid Accumulation and Gene Expression in the Model Green Alga Chlamydomonas reinhardtii
page: 453 - 461
Author:Pornpan Napaumpaiporn and Anchalee Sirikhachornkit*
Vol.43 No.3 (APRIL 2016) View: 709 Download:537
Analysis of Chitin, Chitosan, and Optimization for Carotenoids Extraction Yield with Rice Bran Oil from Thai Fairy Shrimp
page: 918 - 929
Author:Tanyarat Poothawan and Khomsorn Lomthaisong
Vol.42 No.4 (OCTOBER 2015) View: 707 Download:229
Medium Optimization for b-Galactosidase Production by a Thermotolerant Yeast
page: 840 - 849
Author:Sirinda Am-aiam and Chartchai Khanongnuch
Vol.42 No.4 (OCTOBER 2015) View: 1,147 Download:246
Enhanced of High Level of b-xylosidase with b-xylanase Production by Co-culturing of Bacillus Strains from Rice Straw using Response Surface Methodology
page: 822 - 839
Author:Chanaporn Trakunjae, Sukhumaporn Sukkhum and Vichien Kitpreechavanich
Vol.42 No.4 (OCTOBER 2015) View: 666 Download:215
Detention Time Study of Algal Biomass Production with Natural Water Medium
page: 549 - 559
Author:Rameshprabu Ramaraj, David Da-Wei Tsai and Paris Honglay Chen
Vol.42 No.3 (JULY 2015) View: 674 Download:230
Improvement of production and stability of silk degumming protease by Bacillus sp. C4 SS-2013
page: 599 - 613
Author:Nisa Romsomsa, Patoomporn Chim-anage, Sarote Sirisansaneeyakul
Vol.42 No.3 (JULY 2015) View: 1,088 Download:220
Statistical Optimization for the Production of D-Mannose Isomerase by Saccharothrix sp. CMU-K747 Using Response Surface Methodology
page: 52 - 61
Author:ApiradeeSiangsuepchart [a] and SaisamornLumyong*[a, b]
Vol.42 No.1 (JANUARY 2015) View: 715 Download:253
β-Carotene Production by Sporobolomyces pararoseus TISTR5213 Using Crude Glycerol as the Sole Carbon Source
page: 17 - 33
Author:Atchara Manowattana [a], Charin Techapun [b], Phisit Seesuriyachan [b], Prasert Hanmoungjai [b] and
Vol.42 No.1 (JANUARY 2015) View: 681 Download:183
Modeling and Optimization of Removal of Rhodamine – B from Wastewaters by Adsorption on Modified Clay
page: 1230 - 1240
Author:Morteza Bahram, Robabeh Talebi, Abdolhossein Naseri* , Sirous Nouri
Vol.41 No.5/2 (OCTOBER 2014) View: 1,177 Download:401
Minimal Changes in the Right Hand Side Data for Correcting Linear Infeasibility Arising in Intensity-Modulated Radiation Therapy with the Generalized Newton Method
page: 1439 - 1443
Author:Saeed Ketabchi [a], Hossein Moosaei [b] and Ying Xiao [c]
Vol.41 No.5/2 (OCTOBER 2014) View: 705 Download:199
Optimization of Solid-state Fermentation for Fruiting Body Growth and Cordecepin Production by Cordyceps militaris
page: 858 - 872
Author:Ting-chi Wen, Cuang-rong Li, Ji-chuan Kang*,Chao Kang and Kevin D. Hyde
Vol.41 No.4 (SPECIAL ISSUE 1) View: 826 Download:533
Chlorophyll is not accurate measurement for algal biomass
page: 547 - 555
Author:Rameshprabu Ramaraj **[a], David D-W. Tsai [a] and Paris Honglay Chen *[a]
Vol.40 No.4 (OCTOBER 2013) View: 676 Download:258
Optimization of Media Components and Operating Conditions for Exogenous Production of Fungal L-asparaginase
page: 270 - 279
Author:Gurunathan Baskar [a], and Sahadevan Renganathan*[b]
Vol.38 No.2 (APRIL 2011) View: 705 Download:163
Optimization of Micro Metal Injection Molding with Multiple Performance Characteristics using Grey Relational Grade
page: 231 - 241
Author:Mohd H.I. Ibrahim*[a,b], Norhamidi Muhamad [b], Abu B. Sulong [b], Khairur R. Jamaludin [c], Nor H.
Vol.38 No.2 (APRIL 2011) View: 681 Download:202
A Comparative Analysis of Conjugate Gradient Algorithms & PSO Based Neural Network Approaches for Reusability Evaluation of Procedure Based Software Systems
page: 123 - 135
Author:Parvinder S. Sandhu*[a], Shalini Chhabra [b]
Vol.38 (SPECIAL ISSUE 2011) View: 735 Download:190
Experiments and Statistical Analysis of Supercritical Carbon Dioxide Extraction
page: 109 - 115
Author:Wipawee Yothipitak, Praiya Thana, Motonobu Goto and Artiwan Shotipruk
Vol.35 No.1 (JANUARY 2008) View: 678 Download:227
Sensitivity Enhancement of Differential Pulse Polarographic Technique by Simplex Optimization Method
page: 71 - 81
Author:Buabarn Poonperm, Waret Veerasai* and Prapin Wilairat
Vol.27 No.2 (DECEMBER 2000) View: 671 Download:187



Search in this journal


Document Search


Author Search

A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z

Popular Search






Chiang Mai Journal of Science

Faculty of Science, Chiang Mai University
239 Huaykaew Road, Tumbol Suthep, Amphur Muang, Chiang Mai 50200 THAILAND
Tel: +6653-943-467




Faculty of Science,
Chiang Mai University




EMAIL
cmjs@cmu.ac.th




Copyrights © Since 2021 All Rights Reserved by Chiang Mai Journal of Science