Screening and Optimization of Squalene Production from Microalgae Aurantiochytrium sp.
Aeujkom Saengwong, Wichien Yongmanitchai and Duenrut Chonudomkul** Author for corresponding; e-mail address: fscidrc@ku.ac.th
Volume: Vol.45 No.2 (March 2018)
Research Article
DOI:
Received: 15 November 2016, Revised: -, Accepted: 24 March 2017, Published: -
Citation: Saengwong A., Yongmanitchai W. and Chonudomkul D., Screening and Optimization of Squalene Production from Microalgae Aurantiochytrium sp., Chiang Mai Journal of Science, 2018; 45(2): 680-691.
Abstract
Microalgae are an alternative potential source of squalene because they grow rapidly, relatively easy to culture and accumulate large amounts of squalene. The objectives of this research are to isolate and screen squalene producing heterotrophic microalgae from mangrove forests of Thailand and to optimize culture conditions. Five hundred and eighty-five strains of the microalgae were isolated from the mangrove forests along the gulf of Thailand and southern, Thailand. After screening, the isolate S02-459 was selected for further studied due to its high squalene content. Sequencing of partial 18S rRNA gene revealed that it belong to the genus Aurantiochytrium. Evaluation of eight factors influencing growth and squalene accumulation, i.e., glucose concentration, yeast extract, peptone, monosodium glutamate, pH, shaking speed, salinity and temperature were carried out by using Plackett-Burman design. Four factors, namely, glucose concentration, yeast extract concentration, peptone concentration and shaking speed were significantly (p<0.05) affected biomass and squalene production. Central Composite design was employed for optimized these four parameters for enhancement the squalene production. These factors were assessed using quadratic model. The coefficient of determination (R2) of more than 0.9 and p-value less than 0.05 indicated that the model was acceptable. The optimized conditions from CCD showed that 30 g/L of glucose, 5.0 g/L of yeast extract, 20 g/L of peptone and shaking speed at 75 rpm were appropriate. Verifications of the predicted values were 41.19±1.86 mg/L of squalene production and 10.85±0.10 mg/g cell dry weight of squalene content. The deviation of experimental values and predicted values for squalene production and content were -1.81% and -6.78%, respectively.
Related Articles
DOI: 10.12982/CMJS.2025.076.
Le Anh Pham, Thi Oanh Doan, Lan Phuong Doan, Thi My Nguyen, Thi Anh Nguyet Nguyen, Phuong Thao Pham, Thi Quynh Hoang, Thi Phuong Quynh Le, Anh Thao Nguyen, Thi Thu Lien Nguyen, Anh Tu Tran and Thi Thuy Duong
Vol.52 No.5 (September 2025)Research Article View: 933 Download: 221
DOI: 10.12982/CMJS.2024.065.
Ramesh Basnet, Anping Du, Li Tan, Ling Guo, Yanglin Jin, Zhuolin Yi, Tianfang Huang, Yang Fang and Hai Zhao
Vol.51 No.4 (July 2024)Research Article View: 1,500 Download: 653
DOI: 10.12982/CMJS.2024.046.
Jutaporn Chanathaworn and Sommai Saramath
Vol.51 No.3 (May 2024)Research Article View: 1,110 Download: 603
DOI: 10.12982/CMJS.2024.027.
Jiraporn Paoaiang, Prukraya Pongyeela, Nirana Chairerk and Juntima Chungsiriporn
Vol.51 No.2 (March 2024)Research Article View: 2,020 Download: 1,824
DOI: 10.12982/CMJS.2024.003.
Jeyakrishnan Sundararaj, Movva Naga Swapna Sri, Anusha Peyyala and Vijayakumar Sivasundar
Vol.51 No.1 (January 2024)Research Article View: 1,962 Download: 2,179
DOI: 10.12982/CMJS.2022.077.
Ekkaphon Jaiyen, Komgrit Leksakul and Nivit Charoenchai
Vol.49 No.4 (July 2022)Research Article View: 1,640 Download: 916
DOI: 10.12982/CMJS.2022.050.
Neelamanie Yapa, Dinuka Lakmali, Krishantha S. De Zoysa, Shalika Silva, Chaminda Manawadu, Bimal M. Herath, Asanka Madhushan, Geethanjana Perera, Oshani Ratnayakae, Ranganathan Kapilan, Ajith Rathnayake, Anura I. Sirisena, Suhail Asad Samantha C. Karunarathna and Chaturanga Bamunuarachchige
Vol.49 No.3 (Special Issue II : May 2022)Review Article View: 2,531 Download: 1,403
page: 607 - 613
Ukrit Jitropas, Chumphol Yunphuttha, Sumeth Siriroj and Worawat Meevasana*
Vol.47 No.4 (Special Issue II : July 2020)Research Article View: 2,371 Download: 1,252
page: 1055 - 1068
Mathurin Vichachucherd, Wichien Yongmanitchai and Duenrut Chonudomkul *
Vol.46 No.6 (November 2019)Research Article View: 1,744 Download: 499
page: 822 - 838
Kubra Eryasar-Orer and Seda Karasu-Yalcin*
Vol.46 No.5 (September 2019)Research Article View: 1,195 Download: 352
page: 714 - 726
Benyong Han, Fang Yin*, Shiqing Liu, Xingling Zhao, Jing Liu, Changmei Wang, Hong Yang, Wudi Zhang*
Vol.46 No.4 (July 2019)Research Article View: 1,251 Download: 650
page: 219 - 235
Lili Liu*, Yuanyuan Meng, Xiaoning Dai and Ke Chen
Vol.46 No.2 (March 2019)Research Article View: 1,179 Download: 335
page: 2597 - 2608
Pornnipa Botthong, Tanatorn Pornsawatchai, Wanticha Savedboworn, Sriwiang Tipkanon, Atipan Siammai and Wiboon Riansa-ngawong
Vol.45 NO.7 (November 2018)Research Article View: 1,353 Download: 304
page: 1030 - 1038
Jessa Marie Millanar, Mark Daniel de Luna, Aummara Yodsa-nga and Kitirote Wantala*
Vol.45 No.2 (March 2018)Research Article View: 1,168 Download: 230
page: 653 - 667
Sureewan Sittijunda and Alissara Reungsang*
Vol.45 No.2 (March 2018)Research Article View: 1,201 Download: 365
page: 106 - 115
Ramaraj Sathasivam, Piyawat Pongpadung, Jantana Praiboon, Anong Chirapart, Savitr Trakulnaleamsai, Sittiruk Roytrakul and Niran Juntawong
Vol.45 No.1 (January 2018)Research Article View: 1,113 Download: 389
page: 144 - 156
Pawinee Deetae*[a, e], Kwanrudee Tseng [a], Chanathinart Pasuphan [a], Atikorn Panya [b], Pawadee Methacanon [c], Waranya Temthawee [a] and Manop Suphantharika [d]
Vol.44 No.1 (JANUARY 2017)Research Article View: 2,498 Download: 418
page: 50 - 58
Wanlapa Lorliam [a], Ancharida Akaracharanya [b], Sukhumaporn Krajangsang [c], Vasana Tolieng [d] a
Vol.44 No.1 (JANUARY 2017)Research Article View: 1,276 Download: 259
page: 1016 - 1026
Sabadee Worrathampitak [a], Shinji Tokuyama [b], Vichien Kitpreechavanich [c] and Sukhumaporn Sukkhum* [a]
Vol.43 No.5 (OCTOBER 2016)Research Article View: 1,210 Download: 372
page: 453 - 461
Pornpan Napaumpaiporn and Anchalee Sirikhachornkit*
Vol.43 No.3 (APRIL 2016)Research Article View: 1,069 Download: 898
page: 840 - 849
Sirinda Am-aiam and Chartchai Khanongnuch
Vol.42 No.4 (OCTOBER 2015)Research Article View: 1,496 Download: 302
page: 549 - 559
Rameshprabu Ramaraj, David Da-Wei Tsai and Paris Honglay Chen
Vol.42 No.3 (JULY 2015)Research Article View: 1,074 Download: 288
page: 599 - 613
Nisa Romsomsa, Patoomporn Chim-anage, Sarote Sirisansaneeyakul
Vol.42 No.3 (JULY 2015)Research Article View: 1,506 Download: 294
page: 52 - 61
ApiradeeSiangsuepchart [a] and SaisamornLumyong*[a, b]
Vol.42 No.1 (JANUARY 2015)Research Article View: 1,159 Download: 322
page: 1230 - 1240
Morteza Bahram, Robabeh Talebi, Abdolhossein Naseri* , Sirous Nouri
Vol.41 No.5/2 (OCTOBER 2014)Research Article View: 1,557 Download: 536
page: 858 - 872
Ting-chi Wen, Cuang-rong Li, Ji-chuan Kang*,Chao Kang and Kevin D. Hyde
Vol.41 No.4 (SPECIAL ISSUE 1)Research Article View: 1,594 Download: 593
page: 547 - 555
Rameshprabu Ramaraj **[a], David D-W. Tsai [a] and Paris Honglay Chen *[a]
Vol.40 No.4 (OCTOBER 2013)Research Article View: 1,078 Download: 306
page: 270 - 279
Gurunathan Baskar [a], and Sahadevan Renganathan*[b]
Vol.38 No.2 (APRIL 2011)Research Article View: 1,076 Download: 243
page: 109 - 115
Wipawee Yothipitak, Praiya Thana, Motonobu Goto and Artiwan Shotipruk
Vol.35 No.1 (JANUARY 2008)Research Article View: 1,144 Download: 319