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Isolation of A Novel Collagenase-producing Strain from Animal Bone Wastes and Optimization of Its Enzyme Production


Paper Type 
 Contributed Paper
Title 
 Isolation of A Novel Collagenase-producing Strain from Animal Bone Wastes and Optimization of Its Enzyme Production
Author 
 Lili Liu*, Yuanyuan Meng, Xiaoning Dai and Ke Chen
Email 
 yangliuyilang@126.com
Abstract:
 A collagenase-producing bacterium was isolated and identified from animal bone
wastes. Based on its morphological, physiological and biochemical characteristics, and 16S
rRNA gene phylogenetic tree analysis, the strain was identified belonging to Bacillus cereus
and named as MBL13. The conditions of culture medium and fermentation were optimized.
The results indicated that sucrose, bone gelatin and Ca2+ were optimal carbon, nitrogen sources
and metal ion for B. cereus MBL13. By response surface methodology (RSM), the optimum
fermentation conditions were made of temperature 33.8 C, fermentation time (X2) of
49.5 h, inoculum concentration (X3) of 45.2 mg/L, medium volume (X4) of 27.3 mL, and
initial pH (X5) of 6.8 with the maximum collagenase activity of 50.03 U/mL. From the
culture supernatant, a novel collagenase was purified and its molecular weight was estimated
by SDS-PAGE to be approximately 52.0 kD. Scanning electron microscopy (SEM)
analysis showed that the purified collagenase could effectively degrade bovine bone collagen.
This study suggested the collagenase produced by B. cereus MBL13 has a great potential as a
novel protease in hydrolyzing animal bone wastes.
Start & End Page 
 219 - 235
Received Date 
 2018-05-26
Revised Date 
Accepted Date 
 2018-10-08
Full Text 
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Keyword 
 animal bone wastes, collagenase, isolation, Bacillus cereus MBL13, optimization
Volume 
 Vol.46 No.2 (March 2019)
DOI 
Citation 
 Liu* L., Meng Y., Dai X. and Chen K., Isolation of A Novel Collagenase-producing Strain from Animal Bone Wastes and Optimization of Its Enzyme Production, Chiang Mai Journal of Science, 2019; 46(2): 219-235.
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