Chiang Mai Journal of Science, Faculty of Science, Chiang Mai University

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Selective Isolation of Cultivable Actinomycetes from Thai Coastal Marine Sediment

Paper Type	Contributed Paper
Title	Selective Isolation of Cultivable Actinomycetes from Thai Coastal Marine Sediment
Author	Pornpan Ruttanasutja and Wasu Pathom-aree
Email	wasu.p@cmu.ac.th
Abstract: This study was designed to improve isolation method for actinomycetes from coastal marine sediments. Five pretreatments, three enrichment media, and fifteen selective media were tested for isolation of actinomycetes from a coastal marine sediment sample from Thailand. The pretreatment methods were (1) Tenfold serial dilution (2) Tenfold serial dilution with 10 minutes shaking at 125 rpm, (3) 1.5% phenol treatment, (4) Shaking sediment suspension for 10 minutes at 125 rpm, and (5) Shaking sediment suspension for 60 minutes at 125 rpm. Three enrichment media were used: (1) Marine Broth (MB), (2) Soil Extract Solution (SES), and (3) Marine Soil Extract Broth (MSB). All culture plates were incubated at 25°C for up to 30 days on 15 selective isolation media. Actinomycetes were isolated only by shaking sediment suspension for 60 minutes at 125 rpm (pretreatment 5). Marine broth was found to promote the isolation of actinomycetes with the highest ratio of total bacteria:actinomycetes (2:1). A total of 209 actinomycetes were isolated. In general, diluted media or low nutrient media were more effective for the isolation of actinomycetes. Molecular identification based on 16S rRNA gene sequences revealed that these isolates belonged to eight known actinomycete genera: Curtobacterium, Dermacoccus, Micromonospora, Microbispora, Pseudonorcardia, Rhodococcus, Streptomyces and Tsukamurella.
Start & End Page	88 - 103
Received Date	2014-04-18
Revised Date
Accepted Date	2014-07-28
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Keyword	pretreatment, enrichment, selective isolation, actinomycete diversity, coastal marine sediment
Volume	Vol.42 No.1 (JANUARY 2015)
DOI
Citation	Ruttanasutja P. and Pathom-aree W., Selective Isolation of Cultivable Actinomycetes from Thai Coastal Marine Sediment, Chiang Mai Journal of Science, 2015; 42(1): 88-103.
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Selective Isolation of Cultivable Actinomycetes from Thai Coastal Marine Sediment

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