Antioxidant and Antimicrobial Properties of Polysaccharides from Rice Brans
Siriluck Surin, Phisit Seesuriyachan, Prodpran Thakeow, SangGuan You and Yuthana Phimolsiripol** Author for corresponding; e-mail address: yuthana.p@cmu.ac.th
Volume: Vol.45 No.3 (May 2018)
Research Article
DOI:
Received: 21 May 2016, Revised: -, Accepted: 6 Febuary 2017, Published: -
Citation: Surin S., Seesuriyachan P., Thakeow P., You S. and Phimolsiripol Y., Antioxidant and Antimicrobial Properties of Polysaccharides from Rice Brans, Chiang Mai Journal of Science, 2018; 45(3): 1372-1382.
Abstract
Four varieties of rice bran [Khao Dawk Mali 105 (KDML), Kor Kho 6 (RD6), Sanpatong 1 (SPT) and Kum Doi Saket] were pretreated with solvents (ethanol and hexane) and the chemical composition, structure, antioxidant and antimicrobial properties of the extracted (crude) polysaccharides were investigated. The polysaccharides contained five functional groups: alkane, alkene, amine, carbonyl and hydroxyl. However, the nature of the solvent and rice bran variety significantly (P < 0.05) effected the polysaccharide chemical composition. Polysaccharides extracted by hexane possessed high antioxidant activity. Kum Doi Saket had the highest polysaccharide content and antioxidant activity. The amount of Kum Doi Saket polysaccharide required to reduce 50% of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzthiazoline-6-sulphonate) (ABTS) radicals (IC50 value) were in the range 1.1-5.2 and 0.6-1.8 mg/mL, respectively. The ferric-reducing antioxidant power (FRAP) of Kum Doi Saket polysaccharide ranged from 28.8-194.4 mmol Fe2+/g. Moreover, the polysaccharides inhibited the pathogenic bacteria, Staphylococcus aureus and Escherichia coli.
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