Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

1,647
Articles
Q3 0.80
Impact Factor
Q3 1.3
CiteScore
7 days
Avg. First Decision
Outline
Figures and Tables
ARTICLE METRICS
Views 1,143
Downloads 677

Extraction of Phenolic Compounds from Fruits of Bitter Melon (Momordica charantia ) with Subcritical Water Extraction and Antioxidant Activities of These Extracts

Parichat Budrat and Artiwan Shotipruk
* Author for corresponding; e-mail address: artiwan.sh@chula.ac.th
Volume: Vol.35 No.1 (JANUARY 2008)
Research Article
DOI:
Received: 17 October 2007, Revised: -, Accepted: 30 October 2007, Published: -

Citation: Budrat P. and Shotipruk A., Extraction of Phenolic Compounds from Fruits of Bitter Melon (Momordica charantia ) with Subcritical Water Extraction and Antioxidant Activities of These Extracts, Chiang Mai Journal of Science, 2008; 35(1): 123-130.

Abstract

Bitter melon (Momordica charantia) is traditionally known for its medicinal properties such as antidiabetic, anticancer, anti-inflammation, antivirus, and cholesterol lowering effects. It contains many phenolic compounds that may have the potential as antioxidant and antimutagen. Although the value of  bitter melon is realized, scientific information on phenolic composition of bitter melon and antioxidant and antimutagenic activities of its extracts from food grade solvents are limited. This study were investigated the total phenolic contents of bitter melon obtained by subcritical water extraction (SCWE) and antioxidant activities of these extracts. The effect of  extraction temperature was considered and the results were compared with the extracts obtained by solvent extraction and soxhlet extraction. The total phenolic contents of bitter melon obtained by the SCWE at 130oC, the SCWE at 150oC, SCWE at 180oC, the SCWE at 200oC, the solvent extraction, and soxhlet extraction were 10.571, 25.219, 42.915, 48.177, 7.743, and 4.992 mg gallic acid equivalents (GAE)/g dry weight (DW), respectively. Overall, the extracts obtained by SCWE were significantly higher than solvent extraction and soxhlet extraction. The main phenolic acid contained in bitter melon was gallic acid. The phenolic acid was calculated from HPLC analysis of the extracts that the gallic acids of the extracts from bitter melon obtained by the SCWE at 130oC, the SCWE at 150oC, SCWE at 180oC, the SCWE at 200oC, the solvent extraction, and soxhlet extraction were 0.0913, 0.3169, 0.5502, 0.6462, 0.0271, and .0120 mg/g DW, respectively. Antioxidant was represented by IC 50 index which the IC50 values of extract obtained with the SCWE at 130oC, the SCWE at 150oC, the SCWE at 180oC, the SCWE at 200oC, the solvent extraction, and soxhlet extraction were 4.480, 3.970, 5.757, 5.720, 8.426, and 6.107 μg/ml, respectively. The IC50 values of  the SCWE at all temperatures were lower than that of  solvent extraction and soxhlet extraction, which means SCWE gave the extracts with higher antioxidantactivity

Keywords: Subcritical water extraction, Bitter melon, Momordica charantia, Antioxidant, Phenolic

Related Articles

Enhancing the Physicochemical Characteristics and Biological Activities of Phenolic-rich ‘Bignay’ (Antidesma bunius (L.) Spreng.) Fruit Beverage by Two-stage Fermentation
DOI: 10.12982/CMJS.2025.011.

Vince Ellison B. Leyeza, Rona Camille M. Lizardo-Agustin, Lloyd Earl L. Flandez and Katherine Ann T. Castillo-Israel

Vol.52 No.2 (March 2025)
Research Article View: 1,429 Download: 414
Effect of Die Temperature and Porang (Amorphophallus mulleri B.) Flour on the Physicochemical Properties, Nutritional Value, Estimated Glycemic Index, and Sensory Evaluation of Brown Rice Snack by a Collet Extruder
DOI: 10.12982/CMJS.2025.003.

Cindytia Selvina Bernas, Sagar Pandappa Kalahal, Domas Galih Patria and Jenshinn Lin

Vol.52 No.1 (January 2025)
Research Article View: 1,864 Download: 382
Microbiological and Biochemical Characterization, and Sensory Evaluation of Dragon Blood Tea Kombucha
DOI: 10.12982/CMJS.2024.088.

Niphawan Panti, Phatthanapong Therdtatha, Suttida Doungta, Supakan Saenkam, Phisit Seesuriyachan, Pornchai Rachtanapun and Kasirawat Sawangrat

Vol.51 No.6 (November 2024)
Research Article View: 1,591 Download: 1,103
Effects of Selected Yeasts on the Chemical Profiles and Antioxidant Activity of Fermented Coffee Beans during the Aging Process
DOI: 10.12982/CMJS.2024.016.

Yi-Ting Chang, Pi-Hui Hsu, Mao-Che Chiu and Jui-Yu Chou

Vol.51 No.1 (January 2024)
Research Article View: 2,044 Download: 543
Evaluation of Alcoholic Extracts of Mucuna pruriens (L.) DC. Var. utilis for Antibacterial, Antioxidant and Cytotoxic Activities toward Human Cancer Cell Lines
DOI: 10.12982/CMJS.2023.045.

Kanokwan Jitpimai, Lukana Ngiwsara, Weeranuch Lang, Thanawan Panichpat, Ratchanee Mingma, Jisnuson Svasti and Jintanart Wongchawalit

Vol.50 No.5 (September 2023)
Research Article View: 2,703 Download: 1,175
Synthesis and Biological Evaluation of Sulfamoyl Carboxamide Derivatives from Sulfur-containing α-Amino acids
DOI: 10.12982/CMJS.2022.070.

Melford C. Egbujor, Uchechukwu C. Okoro, Attah S. Egu, Vivian I. Okonkwo, Sunday N. Okafor, Chigbundu N. Emeruwa, Pius I. Egwuatu, Odera R. Umeh, Mercy I. Eziafakaego, Ifeanyi S. Amasiatu and David C. Nwobodo

Vol.49 No.4 (July 2022)
Research Article View: 2,430 Download: 390
Determination of Both Total Phenolic Compound and Antioxidant Capacity of Herbal Teas Using Smartphone Digital Imaging
DOI: 10.12982/CMJS.2022.030.

Hau Van Nguyen, Jaroon Jakmunee, Napaporn Youngvises and Pakorn Preechaburana

Vol.49 No.2 (March 2022)
Research Article View: 2,000 Download: 1,413
Genetic Diversity Assessment Through Morphological, Biological, and Molecular Studies Among Thai Holy Basil Landraces and Commercial Cultivars
page: 1500 - 1510

Ponsit Sathapondecha and Sukhuman Whankaew

Vol.48 No.6 (November 2021)
Research Article View: 2,459 Download: 781
Antioxidant and Antiproliferative Properties of a Chinese Water Chestnut Extract and Its Biologically Active Isolated Compounds
page: 1036 - 1044

Jinfeng Yang, Myong Jo Kim, Weiling Chen, Yong Soo Kwon and Ji Hye Yoo

Vol.48 No.4 (July 2021)
Research Article View: 1,248 Download: 130
Investigation of Skin Whitening Property of Soybean Seed Protein; Anti-tyrosinase, Anti-inflammation and Antioxidant
page: 150 - 162

Anupong Joompang, Wisarut Payoungkiattikun, Jirayut Dansuk, Napat Nuramrum, Nisachon Jangpromma, Santi Phosri, Bancha Mahong and Sompong Klaynongsruang*

Vol.48 No.1 (January 2021)
Research Article View: 1,828 Download: 1,202
Antioxidant and Anti-inflammatory Activities of Ethanolic Extract from Hoya kerrii Craib
page: 912 - 925

Patcharawan Sittisart, Benjawan Dunkhunthod and Chokchai Chuea-nongthon

Vol.47 No.5 (September 2020)
Research Article View: 1,587 Download: 738
Antioxidant, Anti-tyrosinase, Anti-aging Potentialsand Safety of Arabica Coffee Cherry Extract
page: 930 - 945

Kanokwan Kiattisin*, Nutjeera Intasai, Nichcha Nitthikan, Thananya Nantarat, Kuan-Han Lee, Wei-Chao Lin, Shang-Chian Lue and Pimporn Leelapornpisid

Vol.46 No.5 (September 2019)
Research Article View: 1,406 Download: 659
Antioxidant and Anti-inflammatory Activities of the Siamese Crocodile (Crocodylus Siamensis) Hemoglobin Hydrolysate Derived from Trypsin and Papain Hydrolysis
page: 915 - 929

Jiraporn Lueangsakulthai, Nicholas Michael, Theeranan Temsiripong, Watcharee Khunkitti, Sompong Klaynongsruang, Nisachon Jangpromma*

Vol.46 No.5 (September 2019)
Research Article View: 1,094 Download: 533
Anti-inflammatory, Antioxidant Activities and Safety of Coffea arabica Leaf Extract for Alternative Cosmetic Ingredient
page: 284 - 294

Kanokwan Kiattisin, Nichcha Nitthikan, Worrapan Poomanee, Pimporn Leelapornpisid, Helmut Viernstein and Monika Mueller

Vol.46 No.2 (March 2019)
Research Article View: 1,106 Download: 1,020
Pharmaceutical and Cosmeceutical Biological Activities of Hemp (Cannabis sativa L var. sativa) Leaf and Seed Extracts
page: 180 - 195

Aranya Manosroi, Charinya Chankhampan, Bang-on Kietthanakorn, Warintorn Ruksiriwanich, Puxvadee Chaikul, Korawinwich Boonpisuttinant, Mathukorn Sainakham, Worapaka Manosroi, Theeraphong Tangjai and Jiradej Manosroi

Vol.46 No.2 (March 2019)
Research Article View: 1,940 Download: 661
Phytochemical Investigation and Antioxidant Properties of Different Extracts from Artabotrys harmandii Finet & Gagnep
page: 2338 - 2347

Wararut Bunchareon, Supaporn Pamok, Supap Saenphet and Kanokporn Saenphet

Vol.45 No.6 (September 2018)
Research Article View: 1,135 Download: 459
Comparative GC-MS Analysis, In-vitro Antioxidant and Antimicrobial Activities of the Essential Oils Isolated from the Peel of Omani Lime
page: 1782 - 1795

Amwaj Mohammed Al-Breiki, Huda Mubarak Al-Brashdi, Jamal Nasser Al-Sabahi and Shah Alam Khan

Vol.45 No.4 (July 2018)
Research Article View: 1,406 Download: 365
Chemometric Profiling of Clinacanthus nutans Leaves Possessing Antioxidant Activities using Ultraviolet-visible Spectrophotometry
page: 1519 - 1530

Hui Wen Chong, Khansa Rezaei, Bee Lynn Chew and Vuanghao Lim*

Vol.45 No.3 (May 2018)
Research Article View: 1,106 Download: 377
Antioxidant and Antimicrobial Properties of Polysaccharides from Rice Brans
page: 1372 - 1382

Siriluck Surin, Phisit Seesuriyachan, Prodpran Thakeow, SangGuan You and Yuthana Phimolsiripol*

Vol.45 No.3 (May 2018)
Research Article View: 1,234 Download: 446
Antioxidant Properties of Crocodylus siamensis Blood Components on H2O2-induced Human Skin Fibroblast Cells
page: 1359 - 1371

Nisachon Jangpromma, Khantheeranut Suttee, Santi Phosri, Tinnakorn Theansungnoen, Jiraporn Lueangsakulthai, Wisarut Payoungkiattikun, Sakda Daduang and Sompong Klaynongsruang*

Vol.45 No.3 (May 2018)
Research Article View: 1,290 Download: 492
Strategic Applications and the Challenges of Subcritical Water Extraction Technology in Food Industries
page: 1015 - 1029

Erasto Mlyuka, Martha Mbifile, Shuang Zhang, Zongping Zheng and Jie Chen*

Vol.45 No.2 (March 2018)
Research Article View: 1,051 Download: 629
Effects of Three Drying Treatments on the Polyphenol Content, Antioxidant and Antimicrobial Properties of Syzygium aromaticum Extract
page: 937 - 948

Suat-HianTan* and Md. Rezaul Karim

Vol.45 No.2 (March 2018)
Research Article View: 1,083 Download: 280
Phenolic Compounds and Antioxidant Activities of Four Edible Species During Seed Germination
page: 905 - 916

Arthitaya Kawee-ai, Pimpak Phumat, Siriporn Okonogi, Srikarnjana Klayraung and Phisit Seesuriyachan*

Vol.45 No.2 (March 2018)
Research Article View: 1,211 Download: 339
Antioxidant, Anti-tyrosinase and Immunomodulatory Activities of the Enzymatic Boiled Venus Clam Hydrolysate
page: 249 - 262

Chanutchamon Sutthiwanjampa and Sang Moo Kim

Vol.45 No.1 (January 2018)
Research Article View: 1,079 Download: 452
Antibacterial Activity of Thai Medicinal Plant Extracts Against Oral and Gastrointestinal Pathogenic Bacteria and Prebiotic Effect on the Growth of Lactobacillus acidophilus
page: 33 - 44

Suree Nanasombat, Nattakorn Kuncharoen, Benjarat Ritcharoon and Paweena Sukcharoen

Vol.45 No.1 (January 2018)
Research Article View: 1,590 Download: 622
Subcritical Water Extraction Technique for Selective Extraction of Pentacyclic Triterpenoids from Dry Loquat (Eriobotrya japonica) Leaves
page: 1534 - 1547

Erasto Mlyuka, Shuang Zhang, Zongping Zheng, Zhiyong He, Maomao Zeng, Martha Mbifile and Jie Chen*

Vol.44 No.4 (October 2017)
Research Article View: 1,065 Download: 391
In vitro Anti-aging Activities of Crocodile (Crocodylus siamensis) Blood Extracts
page: 1334 - 1346

Jiradej Manosroi, Charinya Chankhampan, Worapaka Manosroi and Aranya Manosroi*

Vol.44 No.4 (October 2017)
Research Article View: 1,222 Download: 340
Antioxidant, Total Phenolics and Total Flavonoid Content of the Aqueous Extract of Caesalpinia bonducella Seeds
page: 929 - 938

Shruti Shukla* and Archana Mehta

Vol.44 No.3 (July 2017)
Research Article View: 1,181 Download: 477
Innovating Botanical Extraction through Application of Microwaves for the Efficient Extraction of Ursolic Acid from Ocimum sanctum Leaves
page: 869 - 880

Rajendra Mehta, Raj Kumar, Roshni Tandey and Vivekananda Mandal*

Vol.44 No.3 (July 2017)
Research Article View: 1,043 Download: 304
Chemical Profiles and Antimicrobial Activities of Thai Propolis Collected from Apis mellifera
page: 438 - 448

Sirikarn Sanpa [a,b], Milena Popova [c], Tawee Tunkasiri [d], Sukum Eitssayeam [d], Vassya Bankova [

Vol.44 No.2 (April 2017)
Research Article View: 1,944 Download: 473
Ultrasonic Extraction, Antioxidant and Anti-glycation Activities of Polysaccharides from Gynura divaricata Leaves
page: 144 - 156

Pawinee Deetae*[a, e], Kwanrudee Tseng [a], Chanathinart Pasuphan [a], Atikorn Panya [b], Pawadee Methacanon [c], Waranya Temthawee [a] and Manop Suphantharika [d]

Vol.44 No.1 (JANUARY 2017)
Research Article View: 2,347 Download: 413
Phenolic Profile, Fatty Acid and Essential Oil Composition Analysis and Antioxidant, Antialzheimer and Antibacterial Activities of Verbascum flavidum Extracts
page: 1090 - 1101

Mehmet Boğa*[a], Abdulselam Ertaş [b], Nesrin Haşimi [c], Serpil Demirci [d], Mustafa Abdullah Yılmaz [e], Hamdi Temel [f] and Ufuk Kolak [g]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,122 Download: 380
Antioxidant Compounds and Activities in Selected Fresh and Blanched Vegetables from Northeastern Thailand
page: 834 - 844

Thidarat Somdee*[a], Udomsak Mahaweerawat [a], Methin Phadungkit [b] and Suneerat Yangyuen [a]

Vol.43 No.4 (JULY 2016)
Research Article View: 1,064 Download: 293
Synthesis, Characterization and Antioxidant Activity of 2-Halobenzoyl Thiourea bearing α- and β-Alanine
page: 578 - 584

Nurziana Ngah*, Nor Azanita Mohamed, and Deny Susanti Darnis

Vol.43 No.3 (APRIL 2016)
Research Article View: 987 Download: 319
Phytochemical Constituents with Antimicrobial and Antioxidant Activities from Xylia xylocarpa (Roxb.) Taub. Sawdust Extracts
page: 11 - 21

Pattarawadee Sumthong Nakmee* [a], Soontree Khuntong [a] and Nitra Nuengchamnong [b]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 1,138 Download: 424
Fatty Acid, Essential Oil and Phenolic Compositions of Alcea pallida and Alcea apterocarpa with Antioxidant, Anticholinesterase and Antimicrobial Activities
page: 89 - 99

Abdulselam Ertas*[a,b], Mehmet Boga [b,c], Isil Gazioglu [d], Yeter Yesil [e] , Nesrin Hasimi [f], Cumali Ozaslan [g], Hasibe Yilmaz[h], Muammer Kaplan[j]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 1,108 Download: 379
Prediction of Antioxidant Capacity of Thai Vegetable Extracts by Infrared Spectroscopy
page: 657 - 668

Trakul Prommajak, Sang Moo Kim, Cheol-Ho Pan, Sang Min Kim, Suthat Surawang, Nithiya

Vol.42 No.3 (JULY 2015)
Research Article View: 1,133 Download: 334
In vitro INHIBITION OF LIPID PEROXIDATION AND THE ANTIOXIDANT SYSTEM OF ENDOPHYTIC FUNGI FROM THAI MEDICINAL PLANTS
page: 429 - 444

Teerayut Theantana [a,b], Duangta Kanjanapothi [c], Saisamorn Lumyong* [b]

Vol.39 No.3 (JULY 2012)
Research Article View: 1,091 Download: 294
Comparative Analysis of Aqueous Extracts of Amaranth and Coriander in Scavenging Free Radical Activity and Protection of DNA against Oxidative Damage
page: 560 - 571

Asmita Dutta and Mukesh Singh

Vol.38 No.4 (OCTOBER 2011)
Research Article View: 1,061 Download: 408
Antioxidant Capacity of Broccoli Seeds Grown in Thailand
page: 117 - 122

Sarunya Chuanphongpanich* [a], Maitree Suttajit [b], Sukon Phanichphant [c Duang Buddhasukh [c]

Vol.33 No.1 (JANUARY 2006)
Research Article View: 1,061 Download: 369
Subcritical Water Extraction of Polyphenolic Compounds from Terminalia chebula Fruits
page: 103 - 108

Rangsriwong P., Rangkadilok N., and Shotipruk A

Vol.35 No.1 (JANUARY 2008)
Research Article View: 1,001 Download: 267
Red Yeast Rice Prepared from Thai Glutinous Rice and the Antioxidant Activities
page: 42 - 49

Em-on Chairote, Griangsak Chairote, and Saisamorn Lumyong

Vol.36 No.1 (JANUARY 2009)
Research Article View: 1,237 Download: 374
Screening of Antioxidants from Some Thai Vegetables and Herbs
page: 1 - 5

Anchana Chanwitheesuk, Nuansri Rakariyatham*, Aphiwat Teerawutgulrag and Teerapol Wongchanapiboon

Vol.29 No.1 (APRIL 2002)
Opinion View: 1,227 Download: 368
Outline
Figures