Paper Type |
Contributed Paper |
Title |
Subcritical Water Extraction Technique for Selective Extraction of Pentacyclic Triterpenoids from Dry Loquat (Eriobotrya japonica) Leaves |
Author |
Erasto Mlyuka, Shuang Zhang, Zongping Zheng, Zhiyong He, Maomao Zeng, Martha Mbifile and Jie Chen* |
Email |
chenjie@jiangnan.edu.cn |
Abstract: Subcritical water extraction (SWE) is a novel alternative to conventional organic solvent extraction method (CE) for isolating corosolic acid (CA) and ursolic acid (UA) from loquat leaves (Eriobotrya japonica). SWE were performed in a subcritical water condition at a system pressure 4-10 MPa with the flow rate 33.33-50.00 mL/min, temperatures 100-250°C, and extraction time 5-90 min. The static mode, dynamic mode and static-dynamic mode for the extraction of CA and UA were optimized separately. LC/MS was employed for the quantification of total pentacyclic triterpenoids (PTTs). The results showed high yields of PTTs (25.02±0.71 mg/g) obtained at SWE conditions of 200°C, 41.67 mL/min and extraction time of 30 min (10 min static mode and 20 min dynamic mode) compared to 4 h extraction with CE (14.39±1.12 mg/g). CA (9.63±0.33 mg/g) and UA (15.04±0.81 mg/g) were the main PTTs obtained from dry loquat leaves at 180°C and 200°C, respectively using static-dynamic mode. Findings from this study suggest that SWE at 150 to 200°C, 10 MPa and 30 to 60 min might be a potential green technology to obtain PTTs from dry loquat leaves. |
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Start & End Page |
1534 - 1547 |
Received Date |
2015-08-18 |
Revised Date |
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Accepted Date |
2015-11-15 |
Full Text |
Download |
Keyword |
corosolic acid, HPLC/MS, loquat leaves, subcritical water extraction, triterpenoids |
Volume |
Vol.44 No.4 (October 2017) |
DOI |
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SDGs |
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