Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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Antioxidant Properties of Crocodylus siamensis Blood Components on H2O2-induced Human Skin Fibroblast Cells

Nisachon Jangpromma, Khantheeranut Suttee, Santi Phosri, Tinnakorn Theansungnoen, Jiraporn Lueangsakulthai, Wisarut Payoungkiattikun, Sakda Daduang and Sompong Klaynongsruang*
* Author for corresponding; e-mail address: somkly@kku.ac.th
Volume: Vol.45 No.3 (May 2018)
Research Article
DOI:
Received: 29 September 2016, Revised: -, Accepted: 16 January 2017, Published: -

Citation: Jangpromma N., Suttee K., Phosri S., Theansungnoen T., Lueangsakulthai J., Payoungkiattikun W., et al., Antioxidant Properties of Crocodylus siamensis Blood Components on H2O2-induced Human Skin Fibroblast Cells, Chiang Mai Journal of Science, 2018; 45(3): 1359-1371.

Abstract

To elucidate the antioxidant activity of Crocodylus siamensis (C. siamensis) blood components on BJ human skin fibroblasts, hydrogen peroxide (H2O2), a reactive oxygen species (ROS) causing cellular injury associated with the induction of numerous diseases, was selected as the oxidant in this research. Using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide solution (MTT) assay, C. siamensis blood components were found to exhibit no cytotoxicity on BJ cells. However, the oxidative damage induced by 500 mM H2O2 to BJ cells led to a significant reduction of cell growth. A pronounced protective effect against this damage was observed upon pre-incubation of the cells with 25, 50 and 100 mg/ml of each C. siamensis blood component for 1 h prior to H2O2-exposure. The highest potential to preserve cell viability was found for C. siamensis hemoglobin (cHb) (89.0%). When observed by visible light microscopy, BJ cells exposed to H2O2 displayed diverse morphological alterations, including decreased cell density, cell shrinkage and loss of typical fibroblast appearance. In contrast, BJ cells pre-incubated with C. siamensis blood components remained largely intact after exposure to H2O2. It could further be shown that treatment with C. siamensis blood components did not exhibit any cytotoxic effect on human peripheral blood mononuclear cells (PBMC). To evaluate if the protective effect of cHb against H2O2-induced damage to fibroblast cells is correlated to cellular apoptosis, annexin V-FITC/PI-staining flow cytometry analysis was conducted. The collected data clearly demonstrate that H2O2 induces apoptosis in human skin fibroblast cells, which was evidently decreased by pre-treatment with cHb. It is therefore concluded that C. siamensis blood components possess a great potential to inhibit H2O2-induced oxidative damage in BJ human skin fibroblast cells.

Keywords: antioxidant, apoptosis, crocodile, hydrogen peroxide, peripheral blood mononuclear cell, plasma, serum, leucocyte, fibroblast

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Vol.44 No.2 (April 2017)
Research Article View: 1,003 Download: 277
Antioxidant Activities and Stability of Seed Kernel Extracts from Mango (Mangifera indica linn.) Cultivated in Northern Thailand
page: 573 - 583

Nichana Nakpanich, Wantida Chaiyana and Pimporn Leelapornpisid*

Vol.44 No.2 (April 2017)
Research Article View: 1,087 Download: 401
Chemical Profiles and Antimicrobial Activities of Thai Propolis Collected from Apis mellifera
page: 438 - 448

Sirikarn Sanpa [a,b], Milena Popova [c], Tawee Tunkasiri [d], Sukum Eitssayeam [d], Vassya Bankova [

Vol.44 No.2 (April 2017)
Research Article View: 1,821 Download: 473
Phytochemical Screening, Phenolic and Flavonoid Contents, Antioxidant and Cytotoxic Activities of Graptophyllum pictum (L.) Griff.
page: 193 - 202

Nadechanok Jiangseubchatveera, Saisunee Liawruangrath, Aphiwat Teerawutgulrag, Dammrong Santiarwor

Vol.44 No.1 (JANUARY 2017)
Research Article View: 1,191 Download: 686
Polyphenol Contents, Antioxidant and Anticancer Activity (MCF-7) of Soybean Products in Thailand
page: 176 - 183

Thidarat Somdee *, Udomsak Mahaweerawat, Jindawan Wibulutai, Namphuang Dungkokruad and Suneerat Yung

Vol.44 No.1 (JANUARY 2017)
Research Article View: 1,092 Download: 1,909
Ultrasonic Extraction, Antioxidant and Anti-glycation Activities of Polysaccharides from Gynura divaricata Leaves
page: 144 - 156

Pawinee Deetae*[a, e], Kwanrudee Tseng [a], Chanathinart Pasuphan [a], Atikorn Panya [b], Pawadee Methacanon [c], Waranya Temthawee [a] and Manop Suphantharika [d]

Vol.44 No.1 (JANUARY 2017)
Research Article View: 2,250 Download: 413
Phenolic Profile, Fatty Acid and Essential Oil Composition Analysis and Antioxidant, Antialzheimer and Antibacterial Activities of Verbascum flavidum Extracts
page: 1090 - 1101

Mehmet Boğa*[a], Abdulselam Ertaş [b], Nesrin Haşimi [c], Serpil Demirci [d], Mustafa Abdullah Yılmaz [e], Hamdi Temel [f] and Ufuk Kolak [g]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,032 Download: 378
Protein Biomarker Screening on Effect of Freeze Dried Crocodile Blood and Vitamin C in Iron-deficient Anemic Rats
page: 1077 - 1088

Ekawit Threenet [a], Win Chaeychomsri [b], Sudawan Chaeychomsri [c] and Jindawan Siruntaweneti* [b]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,393 Download: 429
Antioxidant Compounds and Activities in Selected Fresh and Blanched Vegetables from Northeastern Thailand
page: 834 - 844

Thidarat Somdee*[a], Udomsak Mahaweerawat [a], Methin Phadungkit [b] and Suneerat Yangyuen [a]

Vol.43 No.4 (JULY 2016)
Research Article View: 981 Download: 291
Synthesis, Characterization and Antioxidant Activity of 2-Halobenzoyl Thiourea bearing α- and β-Alanine
page: 578 - 584

Nurziana Ngah*, Nor Azanita Mohamed, and Deny Susanti Darnis

Vol.43 No.3 (APRIL 2016)
Research Article View: 922 Download: 318
Determination of Antioxidant and Anticancer Activities together with Total Phenol and Flavonoid Contents of Cleidion javanicum Bl. and Bridelia retusa (L.) A. Juss.
page: 535 - 546

Duangsuree Sanseera, Boonsom Liawruangrath *, Stephen G. Pyne and Saisunee Liawruangrath*

Vol.43 No.3 (APRIL 2016)
Research Article View: 1,492 Download: 867
Effect of Plasma Power on Copper Substrate Used for Synthesizing Carbon Nanotubes via Alcohol Catalytic Chemical Vapor Deposition (ACVD) Technique
page: 339 - 344

Sureewan Phunwaree*, Wim Nhuapeng, Dheerawan Boonyawan, and Wandee Thamjaree*

Vol.43 No.2 (SPECIAL ISSUE 1)
Research Article View: 926 Download: 294
Characterization of NiO–α-MoO3 Composites Synthesized by Microwave Plasma
page: 276 - 281

Surin Promnopas, Orawan Wiranwetchayan, Titipun Thongtem, Wonchai Promnopas and Somchai Thongtem

Vol.43 No.2 (SPECIAL ISSUE 1)
Research Article View: 916 Download: 420
Phytochemical Constituents with Antimicrobial and Antioxidant Activities from Xylia xylocarpa (Roxb.) Taub. Sawdust Extracts
page: 11 - 21

Pattarawadee Sumthong Nakmee* [a], Soontree Khuntong [a] and Nitra Nuengchamnong [b]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 1,049 Download: 424
Optimization of the Preparation of Gelatin Hydrolysates with Antioxidative Activity from Lizardfish (Saurida spp.) Scales Gelatin
page: 68 - 79

Sutee Wangtueai [a, b]*, Susanne Siebenhandl-Ehn [c] and Dietmar Haltrich [c]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 1,153 Download: 559
Antioxidant Capacity and Phenolic Content Evaluation on Peanut Skins from 3 Peanut Types
page: 123 - 137

Pornnapa Chuenchom, Prasan Swatsitang, Thanaset Senawong, Sanun Jogloy

Vol.43 No.1 (JANUARY 2016)
Research Article View: 1,091 Download: 594
Insights Into The Causes and Anti-hyperglycemic Effects Of Punica granatum Rind in Alloxan-induced Diabetic Rats
page: 112 - 122

Sushil Kumar Middha [a,c], Talambedu Usha [b]* and Veena Pande [a]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 898 Download: 406
Ameliorating Reactive Oxygen Species-Induced in vitro Lipid Peroxidation in Liver, Carbohydrate and DNA Damage by Dendrocalamus hamiltonii Different Leaf Extracts
page: 80 - 88

Arvind Kumar Goyal [a,b], Sushil Kumar Middha [c], Talambedu Usha [c] and Arnab Sen*[a]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 969 Download: 245
Fatty Acid, Essential Oil and Phenolic Compositions of Alcea pallida and Alcea apterocarpa with Antioxidant, Anticholinesterase and Antimicrobial Activities
page: 89 - 99

Abdulselam Ertas*[a,b], Mehmet Boga [b,c], Isil Gazioglu [d], Yeter Yesil [e] , Nesrin Hasimi [f], Cumali Ozaslan [g], Hasibe Yilmaz[h], Muammer Kaplan[j]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 1,017 Download: 379
Comparative Studies on Chemical Composition, Phenolic Compounds and Antioxidant Activities of Brown and White Perilla (Perilla frutescens) Seeds
page: 896 - 906

Sikeret Kongkeaw, Siriporn Riebroy and Manat Chaijan

Vol.42 No.4 (OCTOBER 2015)
Research Article View: 1,051 Download: 349
HPTLC Analysis, Antioxidant, Anti-Inflammatory and Xanthine Oxidase Inhibitory Activity of Plumbago zeylanica L
page: 886 - 895

Shivraj Hariram Nile, Umesh Bapurao Patil and Se Won Park

Vol.42 No.4 (OCTOBER 2015)
Research Article View: 1,006 Download: 514
Prediction of Antioxidant Capacity of Thai Vegetable Extracts by Infrared Spectroscopy
page: 657 - 668

Trakul Prommajak, Sang Moo Kim, Cheol-Ho Pan, Sang Min Kim, Suthat Surawang, Nithiya

Vol.42 No.3 (JULY 2015)
Research Article View: 1,040 Download: 334
Determination of the Effects of Adding Milk and Sugar on the Antioxidant Capacity of Oolong Tea by Chemical and Cell Culture-Based Antioxidant Assays
page: 699 - 711

Angkana Wipatanawin, Sudarat Phongsawanit, Thadthanan Maneeratprasert, Sittiwat Lertsiri

Vol.42 No.3 (JULY 2015)
Research Article View: 1,128 Download: 377
Fatty Acid Composition, Physical Properties, Acute Oral Toxicity and Antioxidant Activity of Crude Lipids from Adipose Tissue of Some Commercialized Freshwater Catfish
page: 626 - 636

Wiwat Wangcharoen, Kriangsak Mengumphan and Doungporn Amornlerdpison

Vol.42 No.3 (JULY 2015)
Research Article View: 954 Download: 265
Juvenile Hormone and Broad-Complex Gene Expression on Programmed Cell Death in the Anterior Silk Glands of the Bamboo Borer (Omphisa fuscidentalis Hampson)
page: 126 - 135

Suparin Bunkuna, Tippawan Singtripop and Manaporn Manaboon

Vol.42 No.1 (JANUARY 2015)
Research Article View: 895 Download: 276
Phenolics production from a novel substrate palm oil mill effluent by Aspergillus niger IBS-103ZA: Evaluation of fermentation conditions and antioxidant activity
page: 1 - 16

Zulkarnain Mohamed Idris, Parveen Jamal, Md Zahangir Alam, Abdul Haseeb Ansari, A

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,023 Download: 309
Plasma Assisted Immobilization of TiO2 nanoparticles onto PLA Surface to Promote the Antimicrobial Activity
page: 1361 - 1374

Chonlada Theerakarunwong*[a], Somruthai Tunma[b] and Dheerawan Boonyawan[c]

Vol.41 No.5/2 (OCTOBER 2014)
Research Article View: 870 Download: 259
In vitro Antioxidant and Antitumor Activities of an Endophytic Fungus Phomopsis liquidambari QH4 from Artemisia annua
page: 992 - 1006

Yixin Qian [a, b], Jichuan Kang*[a, c], Lu Wang [a], Bangxing Lei [a] and Kevin D. Hyde [d, e]

Vol.41 No.5/1 (OCTOBER 2014)
Research Article View: 1,000 Download: 305
Porous Poly(ethylene oxide) / Poly(L-lactic acid) Fiber Fabricated via Coaxial Electrospinning: Potential as Biomedical Applications.
page: 1301 - 1316

Ramaimas Chankoa , Narumol Kreua-ongarjnukool, Pitt Supaphol and Prasit Pavasant

Vol.41 No.5/2 (OCTOBER 2014)
Research Article View: 859 Download: 272
Gastroprotective Effects and Antioxidant Activities of Paederia pilifera Hook.f. Root Extract
page: 1121 - 1131

Kantarat Jirakittirat, Supap Saenphet and Kanokporn Saenphet*

Vol.41 No.5/1 (OCTOBER 2014)
Research Article View: 881 Download: 269
Histone deacetylase inhibitory activity and antiproliferative activity of the cultured medium of Aspergillus niger strain TS1
page: 981 - 991

Thanaset Senawong *[a,d], Somprasong Khaopha [a], Suwatchai Misuna [a], Wandee Bunyatratchata [b], N

Vol.41 No.5/1 (OCTOBER 2014)
Research Article View: 1,012 Download: 278
Some Microstructural Studies of Plasma-sprayed Stainless Steel 316L/Al2O3-TiO2 Produced Using Two-Powder Port Configuration
page: 798 - 803

Phuangphaga Daram [a], Jirasak Sukunta [a], Chalermchai Sukhonket [b], Hathaipat Koiprasert [b] and

Vol.40 No.4 (OCTOBER 2013)
Research Article View: 1,064 Download: 266
Phenolic acids content and antioxidant capacity of fruit extracts from Thailand
page: 636 - 642

Nattaya Ongphimai [a], Supathra Lilitchan*[a], Kornkanok Aryusuk [b], Akkarach Bumrungpert [a], et a

Vol.40 No.4 (OCTOBER 2013)
Research Article View: 1,032 Download: 366
Physical Characteristics and Biological Activities of Thai Flower Extracts Loaded in Niosomes
page: 603 - 617

Aranya Manosroi*[a,b], Bang-on Kietthanakorn [b], Charinya Chankhampan [b], Narinthorn Khositsuntiwo

Vol.40 No.4 (OCTOBER 2013)
Research Article View: 1,000 Download: 361
Role of Quercetin and L-Arginine in Alleviating Zinc Oxide Nanoparticle Hepatotoxicity in Rats
page: 577 - 592

Nayira A. Abdel Baky [a]*, L.M. Faddah [a] , Nouf M. Al-Rasheed [a], Nawal M. Al-Rasheed [a] , and W

Vol.40 No.4 (OCTOBER 2013)
Research Article View: 979 Download: 321
The Potential of Electrospun Poly(L-lactic Acid) Fiber Mats Containing a Crude Garcinia Dulcis Extract for Use as Wound Dressings
page: 517 - 533

Orawan Suwantong *[a], Porntipa Pankongadisak [a], Suwanna Deachathai [a] and Pitt Supaphol [b]

Vol.40 No.3 (JULY 2013)
Research Article View: 965 Download: 312
Development of Sequential Injection Analysis Using Peristaltic Pump and Electrochemical Detection for Antioxidant Capacity Test by ABTS Assay
page: 224 - 231

Jakkrapun Kongkedsuk [a], Apisake Hongwitayakorn [a], Wannarekha Bootnapang [b], Nisachon Boonto [b

Vol.40 No.2 (APRIL 2013)
Research Article View: 1,407 Download: 338
Antimicrobial and Antioxidant Activities of Methanol, Dichloromethane and Ethyl Acetate Extracts of Scutellaria lindbergii Rech.f.
page: 49 - 59

Bibi Sedigheh Fazly-Bazzaz *[a], Atefeh Arab [b], Seyed Ahmad Emami [c], Javad Asili [c], Mohamad Ha

Vol.40 No.1 (JANUARY 2013)
Research Article View: 978 Download: 333
Antioxidant Potential of Pleurotus porrigens Extract and Application in Sunflower Oil during Accelerated Storage
page: 34 - 48

Hip Seng Yim*[a, b], Fook Yee Chye [b], Mei Lian Liow [a], and Chun Wai Ho [a]

Vol.40 No.1 (JANUARY 2013)
Research Article View: 1,014 Download: 359
Antioxidant and Sensory Properties of Thai Herbal Teas with emphasis on Thunbergia laurifolia Lindl.
page: 599 - 609

Eric W.C. Chan*, Suit Ying Eng, Yuen Ping Tan, Zhiew Cheng Wong, Phui Yan Lye, and Lea Ngar Tan

Vol.39 No.4 (OCTOBER 2012)
Research Article View: 1,176 Download: 353
Nutritional and Physicochemical Qualities of Thua Nao (Thai Traditional Fermented Soybean)
page: 562 - 574

Katekan Dajanta1,2, Ekachai Chukeatirote3,* and Arunee Apichartsrangkoon2

Vol.39 No.4 (OCTOBER 2012)
Research Article View: 928 Download: 342
High Subzero Temperature Preservation of Spirulina platensis (Atrhrospira fusiformis) and Its Ultrastucture
page: 554 - 561

Manita Motham, Yuwadee Peerapornpisal, Sudaporn Tongsriri, Chayakorn Pumas and Panmuk Vacharapiyasop

Vol.39 No.4 (OCTOBER 2012)
Research Article View: 769 Download: 273
Antioxidant, Antibacterial, Anticancer Activities and Chemical Constituents of the Essential Oil from Mesua ferrea Leaves
page: 455 - 463

Sukanya Keawsa-ard* [a] and Samart Kongtaweelert [b]

Vol.39 No.3 (JULY 2012)
Research Article View: 1,034 Download: 626
In vitro INHIBITION OF LIPID PEROXIDATION AND THE ANTIOXIDANT SYSTEM OF ENDOPHYTIC FUNGI FROM THAI MEDICINAL PLANTS
page: 429 - 444

Teerayut Theantana [a,b], Duangta Kanjanapothi [c], Saisamorn Lumyong* [b]

Vol.39 No.3 (JULY 2012)
Research Article View: 986 Download: 294
Comparative Analysis of Aqueous Extracts of Amaranth and Coriander in Scavenging Free Radical Activity and Protection of DNA against Oxidative Damage
page: 560 - 571

Asmita Dutta and Mukesh Singh

Vol.38 No.4 (OCTOBER 2011)
Research Article View: 968 Download: 404
Antiproliferative Activity of Berberine from Coscinium fenestratum on NCI-H838 Cell Line
page: 85 - 94

Rudeewan Tungpradit, Supachok Sinchaikul, Suree Phutrakul, Weerah Wongkham, Shui-Tein Chen

Vol.38 No.1 (JANUARY 2011)
Research Article View: 1,397 Download: 278
Self-Diffusion Coefficient of Liquid Alkali Metals
page: 125 - 129

Sant K. Srivastava* [a] and Kishor G. Kolhe [b]

Vol.31 No.2 May 2004
Research Article View: 988 Download: 220
Antioxidant Capacity of Broccoli Seeds Grown in Thailand
page: 117 - 122

Sarunya Chuanphongpanich* [a], Maitree Suttajit [b], Sukon Phanichphant [c Duang Buddhasukh [c]

Vol.33 No.1 (JANUARY 2006)
Research Article View: 976 Download: 367
Extraction of Phenolic Compounds from Fruits of Bitter Melon (Momordica charantia ) with Subcritical Water Extraction and Antioxidant Activities of These Extracts
page: 123 - 130

Parichat Budrat and Artiwan Shotipruk

Vol.35 No.1 (JANUARY 2008)
Research Article View: 1,018 Download: 677
Preliminary Screening of Glycoprotein Profiles in Human Normal and Lung Cancer Sera by Different Staining Methods
page: 331 - 344

Piyorot Hongsachart, Supachok Sinchaikul, Suree Phutrakul and Shui-Tein Chen

Vol.35 No.2 (MAY 2008)
Research Article View: 870 Download: 248
Proteomic Analysis of Deglycosylated Proteins in Normal Human Serum Using Anhydrous Hydrogen Fluoride Treatment
page: 311 - 323

Supawadee Sriyam, Supachok Sinchaikul, Payungsak Tantipaiboonwong, Suree Phutrakul and Shui-Tein Che

Vol.35 No.2 (MAY 2008)
Research Article View: 914 Download: 254
Red Yeast Rice Prepared from Thai Glutinous Rice and the Antioxidant Activities
page: 42 - 49

Em-on Chairote, Griangsak Chairote, and Saisamorn Lumyong

Vol.36 No.1 (JANUARY 2009)
Research Article View: 1,155 Download: 374
Theoretical Study of the Bombyx mori Silk Surface Functionalization: Quantum Mechanical Calculation of the Glycine-Alanine Unit Reacting with Fluorine and Molecular Dynamic Simulation of Wettability
page: 106 - 115

Padungsee Khomhoi, Waleepan Sangprasert, Vannajan S. Lee, and Piyarat Nimmanpipug

Vol.37 No.1 (JANUARY 2010)
Research Article View: 975 Download: 299
Screening of Antioxidants from Some Thai Vegetables and Herbs
page: 1 - 5

Anchana Chanwitheesuk, Nuansri Rakariyatham*, Aphiwat Teerawutgulrag and Teerapol Wongchanapiboon

Vol.29 No.1 (APRIL 2002)
Opinion View: 1,133 Download: 366
Antioxidant and Anti-inflammatory Activities of Brown Marine Alga, Padina minor Yamada
page: 507 - 516

Yuwadee Peerapornpisal, Doungporn Amornlerdpison, Utan Jamjai, Tawat Taesotikul, Yanee Pongpaibul,

Vol.37 No.3 (SEPTEMBER 2010)
Research Article View: 1,038 Download: 375
In vitro Antioxidant Activity of Vitex negundo L. Leaf Extracts
page: 489 - 497

Raghavendra H. Lakshmanashetty, Vijayananda B. Nagaraj, Madhumathi G. Hiremath and Vadlapudi Kumar

Vol.37 No.3 (SEPTEMBER 2010)
Research Article View: 1,535 Download: 1,186
Stability Study of Six 1,4-Benzodiazepines in Bio-fluids Stored at -20oC
page: 451 - 463

Mohammad N. Uddin, Victoria F. Samanidou, and Ioannis N. Papadoyannis

Vol.37 No.3 (SEPTEMBER 2010)
Research Article View: 1,015 Download: 525
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