Determination of Antioxidant and Anticancer Activities together with Total Phenol and Flavonoid Contents of Cleidion javanicum Bl. and Bridelia retusa (L.) A. Juss.

Duangsuree Sanseera; Boonsom Liawruangrath; Stephen G. Pyne; Saisunee Liawruangrath

Determination of Antioxidant and Anticancer Activities together with Total Phenol and Flavonoid Contents of Cleidion javanicum Bl. and Bridelia retusa (L.) A. Juss.

Duangsuree Sanseera, Boonsom Liawruangrath *, Stephen G. Pyne and Saisunee Liawruangrath*
* Author for corresponding; e-mail address: liawruangrath@gmail.com; scislwrn@gmail.com
Volume: Vol.43 No.3 (APRIL 2016)
Research Article
DOI:
Received: 28 July 2014, Revised: -, Accepted: 11 November 2014, Published: -

Citation: Sanseera D., Liawruangrath B., Pyne S.G. and Liawruangrath S., Determination of Antioxidant and Anticancer Activities together with Total Phenol and Flavonoid Contents of Cleidion javanicum Bl. and Bridelia retusa (L.) A. Juss., Chiang Mai Journal of Science, 2016; 43(3): 535-546.

Abstract

The antioxidant and anticancer activities of the hexane, chloroform and methanol extracts of the leaves, stems and fruits from Cleidion javanicum Bl. and Bridelia retusa (L.) A. Juss. were investigated. The antioxidant activities were evaluated by DPPH and ABTS methods. The methanol extracts of the stems and fruits from B. retusa and the methanol extract of the leaves from C. javanicum showed the highest antioxidant activity. The methanol extract of the leaves from B. retusa possessed significant anticancer activity against KB-Oral cavity cancer (IC₅₀ = 21.24 µg/mL), MCF-7 breast cancer cell (IC₅₀ = 17.81 µg/mL), and NCI-H 187 – small cell lung cancer (IC₅₀ = 24.24 µg/mL), whereas the methanol extract of the fruits exhibited the highest anticancer activity against NCI-H 187 – small cell lung cancer with an IC₅₀ value of 8.21 µg/mL. The hexane extract of the fruits from C. javanicum showed significant anticancer activity against NCI-H 187 – small cell lung cancer with an IC₅₀ value of 29.11 µg/mL. All of the extracts of these two medicinal plants were non-cytotoxic to primate cell line (Vero cell). Quantitative determination of phenols and flavonoids in leaves, stems and fruits extracts from B. retusa and C. javanicum was carried out using spectrophotometric methods. The methanol extract of B. retusa fruits and the methanol extract of C. javanicum stems contained the highest phenolic content. The methanol extract of B. retusa leaves contained maximum flavonoid, whereas the chloroform extract of C. javanicum leaves contained maximum flavonoid. These two plants may play important roles for the production of health supplement and might be led to the isolation of lead compounds for drug development.

Keywords: anticancer activity, antioxidant activity, total phenol and flavonoid contents, Cleidion javanicum, Bridelia retusa

Comparative Effects of Solvent Polarity on Phenolic and Flavonoid Contents, Antioxidant Activity, and Cytotoxicity of Litsea elliptica Leaf Extracts
DOI: 10.12982/CMJS.2026.067.

Kwanchayanawish Machana*, Anusorn Thumpithak, Sirirat Phakpaknam, Pattarakorn Sirikul, Pornpimol Namvichaisirikul, Atcharee Chatsuwankit, Nitcha Prapruttipong, Wuttipat Kumpiranont, Kanokwan Waiyanont, Atthasit Sangthong, Natcha Namsiripongpun, Chonlada Judprakob and Natthida Weerapreeyakul

Vol.53 No.4 (July 2026)
Research Article View: 379 Download: 45

Effects of Gum Arabic-Calcium Chloride Composite Coating on Postharvest Quality and Antioxidant Properties of Cold-Stored Tarap (Artocarpus odoratissimus)
DOI: 10.12982/CMJS.2026.054.

Nuratika Tamimi Sheikh Mohamed*, Clayrieysa Samantha Michael Solomon, Shiamala Devi Ramaiya, Hanisah Kamilah, Noorasmah Saupi and Khairunnasrin Binti Hamzah

Vol.53 No.3 (May 2026)
Research Article View: 373 Download: 49

Development and Evaluation of Buckwheat-Fortified Mung Bean Extrudates: Effects on Physicochemical, Textural, and Antioxidant Properties
DOI: 10.12982/CMJS.2025.100.

Girish Ningappa Mathad and Jenshinn Lin

Vol.52 No.6 (November 2025)
Research Article View: 675 Download: 122

Exploring the Effect of Incubation pH, Temperature, and In Vitro Digestion on Antioxidant Potential of Mung Bean Protein Hydrolysates
DOI: 10.12982/CMJS.2024.090.

Zohreh Karami and Kiattisak Duangmal

Vol.51 No.6 (November 2024)
Research Article View: 1,224 Download: 330

Effects of Simulated Digestion on the Bioaccessibility of Phenolic Compounds and Antioxidant Activity of Dehydrated Bignay [Antidesma bunius (L.) Spreng]
DOI: 10.12982/CMJS.2024.032.

Ara Fatima A. Carbonera, Liezl M. Atienza, Maria Amelita C. Estacio, Sheba Mae M. Duque, Rona Camille M. Lizardo-Agustin, and Katherine Ann T. Castillo-Israel

Vol.51 No.2 (March 2024)
Research Article View: 1,966 Download: 521

Effects of Aqueous Extraction Factors on Commercial Protease Inhibitory Activities, Phenolic Acid Profiles and Chemical Properties of Hom Mali-105 Rice (Oryza sativa L.) Bran Extract
DOI: 10.12982/CMJS.2023.054.

Worachai Wongwatcharayothin, Supawan Thawornchinsombut and Akkasit Jongjareonrak

Vol.50 No.5 (September 2023)
Research Article View: 1,820 Download: 960

Efficacy of Dried Indian Gooseberry Kombucha on a Pre–industrial Scale: Chemical Composition, Antioxidant and Antibacterial Activity
DOI: 10.12982/CMJS.2023.034.

Tharinee Klawpiyapamornkun, Thida Kaewkod, Toungporn Uttarotai, Sunanta Wangkarn, Panee Sirisa-ard, Suwalee Kiatkarun, Yingmanee Tragoolpua and Sakunnee Bovonsombut

Vol.50 No.3 (May 2023)
Research Article View: 1,734 Download: 831

Cytotoxicity of Aromatic Substituted Curcuminoid against Human Lung Cancer Cell Line
page: 1313 - 1321

Hien Van Nguyen, Van Thi Bich Pham and Hao Minh Hoang

Vol.48 No.5 (September 2021)
Research Article View: 1,744 Download: 704

Potent Antioxidant Activities of Half-Sib Families of Eucalyptus camaldulensis Dehnh. Leaf Essential Oils Planted in Thailand and Their Antioxidative Components
page: 112 - 122

Sapit Diloksumpun, Preeya Jeenho, Samran Namkhot, Tharinee Saleepochn, Suwaporn Luangkamin*

Vol.48 No.1 (January 2021)
Research Article View: 1,602 Download: 555

Multi-response Optimization of Ultrasonic Assisted Enzymatic Extraction of Antioxidant Polysaccharides from Waste Ginkgo biloba Sarcotesta
page: 499 - 512

Gui-Sheng Zhou, Yu-Ping Tang, Yao Li and Jin-Ao Duan

Vol.47 No.3 (May 2020)
Research Article View: 1,360 Download: 2,124

Solvent Extraction and Measurement of Antioxidant Activity and Total Phenolic Content from Capsicum chinense Jacq. Cv Habanero at Different Maturity Stages
page: 661 - 671

Víctor Manuel Moo-Huchin*, María de Lourdes Vargas y Vargas , Jorge Abraham Tamayo-Cortez, Daniel Arcángel López-Sauri, Enrique Sauri-Duch, Alejandro Ortiz-Fernández, César Abraham Can-Cauich, David Betancur-Ancona

Vol.46 No.4 (July 2019)
Research Article View: 1,262 Download: 455

Optimization of Ultrasonic-assisted Extraction Using Taguchi Design and In vivo Activities ofPolysaccharides from Flos populi
page: 510 - 522

Ying Zhang, Jiaxuan Li, Zheng Jia, Jianqing Chen, Chunbo Gao and Zunlai Sheng

Vol.46 No.3 (May 2019)
Research Article View: 1,216 Download: 346

Antibacterial, Antioxidant Properties and Bioactive Compounds of Thai Cultivated Mushroom Extracts Against Food-borne Bacterial Strains
page: 1713 - 1727

Mathurot Chaiharn, Waya S. Phutdhawong, Doungporn Amornlerdpison and Weerachai Phutdhawong

Vol.45 No.4 (July 2018)
Research Article View: 1,434 Download: 552

Antibacterial and Antioxidant Activities of 3-O-methyl Ellagic Acid from Stem Bark of Polyalthia longifolia Thw.
page: 858 - 867

Pankaj Kumar Jain, Arjun Patra*+, Swaha Satpathy, Sonika Jain and Saleemulla Khan

Vol.45 No.2 (March 2018)
Research Article View: 1,266 Download: 369

Antioxidant Activity and Starch-digesting Enzyme Inhibition of Selected Thai Herb Extracts
page: 263 - 276

Krittalak Pasakawee, Somdet Srichairatanakool, Thunnop Laokuldilok and Niramon Utama-ang

Vol.45 No.1 (January 2018)
Research Article View: 1,318 Download: 304

Carotenoid Composition and Antioxidant Activity of Extracts From Tropical Fruits
page: 605 - 616

V ctor M. Moo-Huchin [a], Gustavo A. Gonz lez-Aguilar [b], Mariela Moo-Huchin [c], Elizabeth Ortiz-V zquez [d], Luis Cuevas-Glory [d], Enrique Sauri-Duch [d] and David Betancur-Ancona* [e]

Vol.44 No.2 (April 2017)
Research Article View: 1,217 Download: 1,061

Antioxidant and Antimicrobial Properties of a Brown Alga Scytosiphon lomentaria
page: 595 - 604

Xiao Juan Xu, Yu Zhang, Hong Lin, Chao Kang, Xiao Ting Fu*

Vol.44 No.2 (April 2017)
Research Article View: 1,211 Download: 285

Antioxidant Activities and Stability of Seed Kernel Extracts from Mango (Mangifera indica linn.) Cultivated in Northern Thailand
page: 573 - 583

Nichana Nakpanich, Wantida Chaiyana and Pimporn Leelapornpisid*

Vol.44 No.2 (April 2017)
Research Article View: 1,381 Download: 406

Phytochemical Screening, Phenolic and Flavonoid Contents, Antioxidant and Cytotoxic Activities of Graptophyllum pictum (L.) Griff.
page: 193 - 202

Nadechanok Jiangseubchatveera, Saisunee Liawruangrath, Aphiwat Teerawutgulrag, Dammrong Santiarwor

Vol.44 No.1 (JANUARY 2017)
Research Article View: 1,525 Download: 717

Polyphenol Contents, Antioxidant and Anticancer Activity (MCF-7) of Soybean Products in Thailand
page: 176 - 183

Thidarat Somdee *, Udomsak Mahaweerawat, Jindawan Wibulutai, Namphuang Dungkokruad and Suneerat Yung

Vol.44 No.1 (JANUARY 2017)
Research Article View: 1,313 Download: 1,914

Essential Oil of Solanum spirale Fruits and Its Biological Activities
page: 547 - 555

Sukanya Keawsa-ard [a], Boonsom Liawruangrath* [b], Saisunee Liawruangrath [c], Aphiwat Teerawutgulrag [c] Stephen G. Pyne [d]

Vol.43 No.3 (APRIL 2016)
Research Article View: 1,720 Download: 375

Optimization of the Preparation of Gelatin Hydrolysates with Antioxidative Activity from Lizardfish (Saurida spp.) Scales Gelatin
page: 68 - 79

Sutee Wangtueai [a, b]*, Susanne Siebenhandl-Ehn [c] and Dietmar Haltrich [c]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 1,417 Download: 564

Comparative Studies on Chemical Composition, Phenolic Compounds and Antioxidant Activities of Brown and White Perilla (Perilla frutescens) Seeds
page: 896 - 906

Sikeret Kongkeaw, Siriporn Riebroy and Manat Chaijan

Vol.42 No.4 (OCTOBER 2015)
Research Article View: 1,259 Download: 355

Fatty Acid Composition, Physical Properties, Acute Oral Toxicity and Antioxidant Activity of Crude Lipids from Adipose Tissue of Some Commercialized Freshwater Catfish
page: 626 - 636

Wiwat Wangcharoen, Kriangsak Mengumphan and Doungporn Amornlerdpison

Vol.42 No.3 (JULY 2015)
Research Article View: 1,175 Download: 274

Bioactive compounds from Mesua ferrea stems
page: 185 - 195

Sukanya Keawsa-ard and Boonsom Liawruangrath

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,162 Download: 336

Phenolics production from a novel substrate palm oil mill effluent by Aspergillus niger IBS-103ZA: Evaluation of fermentation conditions and antioxidant activity
page: 1 - 16

Zulkarnain Mohamed Idris, Parveen Jamal, Md Zahangir Alam, Abdul Haseeb Ansari, A

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,199 Download: 314

In vitro Antioxidant and Antitumor Activities of an Endophytic Fungus Phomopsis liquidambari QH4 from Artemisia annua
page: 992 - 1006

Yixin Qian [a, b], Jichuan Kang*[a, c], Lu Wang [a], Bangxing Lei [a] and Kevin D. Hyde [d, e]

Vol.41 No.5/1 (OCTOBER 2014)
Research Article View: 1,221 Download: 314

Histone deacetylase inhibitory activity and antiproliferative activity of the cultured medium of Aspergillus niger strain TS1
page: 981 - 991

Thanaset Senawong *[a,d], Somprasong Khaopha [a], Suwatchai Misuna [a], Wandee Bunyatratchata [b], N

Vol.41 No.5/1 (OCTOBER 2014)
Research Article View: 1,263 Download: 283

Physical Characteristics and Biological Activities of Thai Flower Extracts Loaded in Niosomes
page: 603 - 617

Aranya Manosroi*[a,b], Bang-on Kietthanakorn [b], Charinya Chankhampan [b], Narinthorn Khositsuntiwo

Vol.40 No.4 (OCTOBER 2013)
Research Article View: 1,239 Download: 370

Antimicrobial and Antioxidant Activities of Methanol, Dichloromethane and Ethyl Acetate Extracts of Scutellaria lindbergii Rech.f.
page: 49 - 59

Bibi Sedigheh Fazly-Bazzaz *[a], Atefeh Arab [b], Seyed Ahmad Emami [c], Javad Asili [c], Mohamad Ha

Vol.40 No.1 (JANUARY 2013)
Research Article View: 1,196 Download: 340

Antioxidant Potential of Pleurotus porrigens Extract and Application in Sunflower Oil during Accelerated Storage
page: 34 - 48

Hip Seng Yim*[a, b], Fook Yee Chye [b], Mei Lian Liow [a], and Chun Wai Ho [a]

Vol.40 No.1 (JANUARY 2013)
Research Article View: 1,207 Download: 366

Nutritional and Physicochemical Qualities of Thua Nao (Thai Traditional Fermented Soybean)
page: 562 - 574

Katekan Dajanta1,2, Ekachai Chukeatirote3,* and Arunee Apichartsrangkoon2

Vol.39 No.4 (OCTOBER 2012)
Research Article View: 1,250 Download: 352

Antioxidant, Antibacterial, Anticancer Activities and Chemical Constituents of the Essential Oil from Mesua ferrea Leaves
page: 455 - 463

Sukanya Keawsa-ard* [a] and Samart Kongtaweelert [b]

Vol.39 No.3 (JULY 2012)
Research Article View: 1,386 Download: 634

Anticancer and Antibacterial Activities of the Isolated Compounds from Solanum spirale Roxb. Leaves
page: 445 - 454

Sukanya Keawsa-ard [a], Surapol Natakankitkul [a], Saisunee Liawruangrath [b], Aphiwat Teerawutgulra

Vol.39 No.3 (JULY 2012)
Research Article View: 1,202 Download: 420

Antioxidant and Anti-inflammatory Activities of Brown Marine Alga, Padina minor Yamada
page: 507 - 516

Yuwadee Peerapornpisal, Doungporn Amornlerdpison, Utan Jamjai, Tawat Taesotikul, Yanee Pongpaibul,

Vol.37 No.3 (SEPTEMBER 2010)
Research Article View: 1,264 Download: 386

Chiang Mai Journal of Science

2,448

Q3 1.0

Q2 1.6

7 days

Outline

Figures and Tables