Antibacterial, Antioxidant Properties and Bioactive Compounds of Thai Cultivated Mushroom Extracts Against Food-borne Bacterial Strains

Mathurot Chaiharn; Waya S. Phutdhawong; Doungporn Amornlerdpison; Weerachai Phutdhawong

Antibacterial, Antioxidant Properties and Bioactive Compounds of Thai Cultivated Mushroom Extracts Against Food-borne Bacterial Strains

Mathurot Chaiharn, Waya S. Phutdhawong, Doungporn Amornlerdpison and Weerachai Phutdhawong
* Author for corresponding; e-mail address: mathurot@mju.ac.th
Volume: Vol.45 No.4 (July 2018)
Research Article
DOI:
Received: 15 November 2016, Revised: -, Accepted: 18 April 2017, Published: -

Citation: Chaiharn M., Phutdhawong W.S., Amornlerdpison D. and Phutdhawong W., Antibacterial, Antioxidant Properties and Bioactive Compounds of Thai Cultivated Mushroom Extracts Against Food-borne Bacterial Strains, Chiang Mai Journal of Science, 2018; 45(4): 1713-1727.

Abstract

A fine-dried edible mushroom was extracted and investigated for its antibacterial activity against food-borne pathogenic bacteria, i.e. Bacillus cereus, Enterobacter aerogenes, Escherichia coli, Micrococcus luteus, Proteus vulgaris, Salmonella typhimurium and Staphylococcus aureus. The extracts of Flammulina velutipes, Ganoderma lucidum, Pleurotus ostreatus and Pleurotus pulmonarius inhibited both Gram-positive and Gram-negative bacteria. Water extract of Pleurotus pulmonarius significantly inhibited the growth of Gram-positive, i.e. Bacillus cereus and Micrococcus luteus (15 mm), while Gram-negative, i.e. Escherichia coli and Salmonella typhimurium resisted to most extracts. Only water extract from Pleurotus pulmonarius showed antibacterial activity against all tested bacteria. The minimum inhibitory concentrations (MICs) of Bacillus cereus had the lowest MIC range (1.25-5.00 mg/ml), whereas those of Salmonella typhimurium had the highest MICs (12.5-22.5 mg/ml) in Flammulina velutipes in ethylacetate extract, Ganoderma lucidum in methanol extract, Pleurotus ostreatus in ethylacetate extract and Pleurotus pulmonarius in water extract. Mushroom crude extracts were investigated for antioxidant capacity using the ABTS system. Results showed an effective antioxidant activity against ABTS radicals (91-94 %) and vitamin E derivatives (97 %), respectively. The IC₅₀ values of Ganoderma lucidum, Pleurotus pulmonarius and Flammulina velutipes were 2.81 ± 0.02 mg/ml to 10.57 ± 0.27 mg/ml. Ganoderma lucidum exhibited maximum antioxidant potential as compared to Pleurotus pulmonarius and Flammulina velutipes, respectively. Positive correlations were found between total phenolic content in the mushroom extracts and their antioxidant activities. Water-extracted bioactive compounds produced by Pleurotus pulmonarius were characterized and identified on the basis of ¹H-NMR as a polysaccharide. The study reveals that cultivated mushroom extracts have higher free radical scavenging potential with high levels of antioxidant compounds. These compounds showed broad-spectrum activity, were non-toxic, and might be applicable for human use. Results suggest that cultivated mushrooms may have potential as natural antibacterial and antioxidant properties and could be used as potential natural source for the development of nutraceuticals.

Keywords: Antibacterial activity, Antioxidant activity, Cultivated mushroom, Food-borne pathogenic bacteria, Polysaccharide

Comparative Effects of Solvent Polarity on Phenolic and Flavonoid Contents, Antioxidant Activity, and Cytotoxicity of Litsea elliptica Leaf Extracts
DOI: 10.12982/CMJS.2026.067.

Kwanchayanawish Machana*, Anusorn Thumpithak, Sirirat Phakpaknam, Pattarakorn Sirikul, Pornpimol Namvichaisirikul, Atcharee Chatsuwankit, Nitcha Prapruttipong, Wuttipat Kumpiranont, Kanokwan Waiyanont, Atthasit Sangthong, Natcha Namsiripongpun, Chonlada Judprakob and Natthida Weerapreeyakul

Vol.53 No.4 (July 2026)
Research Article View: 119 Download: 12

Effects of Gum Arabic-Calcium Chloride Composite Coating on Postharvest Quality and Antioxidant Properties of Cold-Stored Tarap (Artocarpus odoratissimus)
DOI: 10.12982/CMJS.2026.054.

Nuratika Tamimi Sheikh Mohamed*, Clayrieysa Samantha Michael Solomon, Shiamala Devi Ramaiya, Hanisah Kamilah, Noorasmah Saupi and Khairunnasrin Binti Hamzah

Vol.53 No.3 (May 2026)
Research Article View: 272 Download: 48

Antibacterial Activity and Photocatalytic Performance of Zinc Oxide Nanoparticles from Morus alba L.
DOI: 10.12982/CMJS.2026.009.

Natrada Phumprakhon, Mongkol Nontakitticharoen, Angkhana Chuajedton, Sirinuch Loiha, Ratchaneekorn Pilasombat, Prawit Nuengmatcha, Surapon Saensouk and Siripit Pitchuanchom*

Vol.53 No.1 (January 2026)
Research Article View: 697 Download: 58

Development and Evaluation of Buckwheat-Fortified Mung Bean Extrudates: Effects on Physicochemical, Textural, and Antioxidant Properties
DOI: 10.12982/CMJS.2025.100.

Girish Ningappa Mathad and Jenshinn Lin

Vol.52 No.6 (November 2025)
Research Article View: 609 Download: 118

Antibacterial Properties of Bacillus sp. TBRC 16324 and Its Efficacy in Controlling Bacterial Leaf Blight Disease in Rice
DOI: 10.12982/CMJS.2024.100.

Juangjun Jumpathong, Chanetsada Yothasuphap, Paweena Permthawee, Bernard Dell, Nungruthai Suphrom, Thanita Boonsrangsom and Tepsuda Rungrat

Vol.51 No.6 (November 2024)
Research Article View: 1,831 Download: 483

Exploring the Effect of Incubation pH, Temperature, and In Vitro Digestion on Antioxidant Potential of Mung Bean Protein Hydrolysates
DOI: 10.12982/CMJS.2024.090.

Zohreh Karami and Kiattisak Duangmal

Vol.51 No.6 (November 2024)
Research Article View: 1,144 Download: 328

Increased Antibacterial Activity of Zinc-Silver Doped Hydroxyapatite Synthesized via Ultrasonic in Combination with Sol-Gel Technique using Glutinous Rice as a Template
DOI: 10.12982/CMJS.2024.044.

Piaw Phatai, Sirilak Kamonwannasit, Sakuntala Siri-udom, Cybelle Morales Futalan, Pongtanawat Khemthong, Saran Youngjan, Teera Butburee, Khongvit Prasitnok and Orrasa Prasitnok

Vol.51 No.3 (May 2024)
Research Article View: 1,258 Download: 601

Effects of Simulated Digestion on the Bioaccessibility of Phenolic Compounds and Antioxidant Activity of Dehydrated Bignay [Antidesma bunius (L.) Spreng]
DOI: 10.12982/CMJS.2024.032.

Ara Fatima A. Carbonera, Liezl M. Atienza, Maria Amelita C. Estacio, Sheba Mae M. Duque, Rona Camille M. Lizardo-Agustin, and Katherine Ann T. Castillo-Israel

Vol.51 No.2 (March 2024)
Research Article View: 1,830 Download: 518

Effects of Aqueous Extraction Factors on Commercial Protease Inhibitory Activities, Phenolic Acid Profiles and Chemical Properties of Hom Mali-105 Rice (Oryza sativa L.) Bran Extract
DOI: 10.12982/CMJS.2023.054.

Worachai Wongwatcharayothin, Supawan Thawornchinsombut and Akkasit Jongjareonrak

Vol.50 No.5 (September 2023)
Research Article View: 1,751 Download: 958

Efficacy of Dried Indian Gooseberry Kombucha on a Pre–industrial Scale: Chemical Composition, Antioxidant and Antibacterial Activity
DOI: 10.12982/CMJS.2023.034.

Tharinee Klawpiyapamornkun, Thida Kaewkod, Toungporn Uttarotai, Sunanta Wangkarn, Panee Sirisa-ard, Suwalee Kiatkarun, Yingmanee Tragoolpua and Sakunnee Bovonsombut

Vol.50 No.3 (May 2023)
Research Article View: 1,684 Download: 828

Cu2O(ClO4)2/TiO2 Dandelion-shaped Nanospheres with Photocatalytic Antibacterial Activity
DOI: 10.12982/CMJS.2022.021.

Xiaolin Xu, Yacong Yang, Yingchun Miao, Kaiquan Liu, Yuqin Chen and Shaoyan Yue

Vol.49 No.2 (March 2022)
Research Article View: 2,118 Download: 438

Potent Antioxidant Activities of Half-Sib Families of Eucalyptus camaldulensis Dehnh. Leaf Essential Oils Planted in Thailand and Their Antioxidative Components
page: 112 - 122

Sapit Diloksumpun, Preeya Jeenho, Samran Namkhot, Tharinee Saleepochn, Suwaporn Luangkamin*

Vol.48 No.1 (January 2021)
Research Article View: 1,571 Download: 552

Multi-response Optimization of Ultrasonic Assisted Enzymatic Extraction of Antioxidant Polysaccharides from Waste Ginkgo biloba Sarcotesta
page: 499 - 512

Gui-Sheng Zhou, Yu-Ping Tang, Yao Li and Jin-Ao Duan

Vol.47 No.3 (May 2020)
Research Article View: 1,343 Download: 2,122

Solvent Extraction and Measurement of Antioxidant Activity and Total Phenolic Content from Capsicum chinense Jacq. Cv Habanero at Different Maturity Stages
page: 661 - 671

Víctor Manuel Moo-Huchin*, María de Lourdes Vargas y Vargas , Jorge Abraham Tamayo-Cortez, Daniel Arcángel López-Sauri, Enrique Sauri-Duch, Alejandro Ortiz-Fernández, César Abraham Can-Cauich, David Betancur-Ancona

Vol.46 No.4 (July 2019)
Research Article View: 1,232 Download: 454

Optimization of Ultrasonic-assisted Extraction Using Taguchi Design and In vivo Activities ofPolysaccharides from Flos populi
page: 510 - 522

Ying Zhang, Jiaxuan Li, Zheng Jia, Jianqing Chen, Chunbo Gao and Zunlai Sheng

Vol.46 No.3 (May 2019)
Research Article View: 1,185 Download: 343

Application of Silver Nanoparticles Synthesized by Using Ipomoea batatas L. Waste to Improve Antibacterial Properties and Hydrophobicity of Polyester Cloths
page: 2715 - 2729

Eli Rohaeti and Anna Rakhmawati

Vol.45 NO.7 (November 2018)
Research Article View: 1,279 Download: 589

Comparative GC-MS Analysis, In-vitro Antioxidant and Antimicrobial Activities of the Essential Oils Isolated from the Peel of Omani Lime
page: 1782 - 1795

Amwaj Mohammed Al-Breiki, Huda Mubarak Al-Brashdi, Jamal Nasser Al-Sabahi and Shah Alam Khan

Vol.45 No.4 (July 2018)
Research Article View: 1,529 Download: 368

Phytochemical Screening and Antibacterial Activities of Tuber Extract of Stephania pierrei Diels
page: 1396 - 1406

Sirilak Kamonwannasit and Agarat Kamcharoen*

Vol.45 No.3 (May 2018)
Research Article View: 1,317 Download: 466

Antioxidant and Antimicrobial Properties of Polysaccharides from Rice Brans
page: 1372 - 1382

Siriluck Surin, Phisit Seesuriyachan, Prodpran Thakeow, SangGuan You and Yuthana Phimolsiripol*

Vol.45 No.3 (May 2018)
Research Article View: 1,401 Download: 456

Antibacterial and Antioxidant Activities of 3-O-methyl Ellagic Acid from Stem Bark of Polyalthia longifolia Thw.
page: 858 - 867

Pankaj Kumar Jain, Arjun Patra*+, Swaha Satpathy, Sonika Jain and Saleemulla Khan

Vol.45 No.2 (March 2018)
Research Article View: 1,243 Download: 367

Antimicrobial Efficacy of a Combination of Crocodile (Crocodylus siamensis) Leukocyte Extract and Hen Egg Lysozyme
page: 797 - 810

Tinnakorn Theansungnoen, Patthana Tastub, Nisachon Jangpromma, Nualyai Yaraksa, Theeranan Temsiripong and Sompong Klaynongsruang*

Vol.45 No.2 (March 2018)
Research Article View: 1,123 Download: 317

Antioxidant Activity and Starch-digesting Enzyme Inhibition of Selected Thai Herb Extracts
page: 263 - 276

Krittalak Pasakawee, Somdet Srichairatanakool, Thunnop Laokuldilok and Niramon Utama-ang

Vol.45 No.1 (January 2018)
Research Article View: 1,281 Download: 302

Newer 1,2,3-oxathiazino [5,6-c] Quinolin-5 (6H) One-2,2-dioxidederivatives: Synthesis, Molecular Properties Prediction and Biological Evaluation as Potent Antibacterial, Anticoagulant Agents
page: 1463 - 1474

Naveen Polkam, Parsharamulu Rayam, Tejeswara Rao Allaka, Shankaraiah Malthum, Naveen Kuntala, Bhaska

Vol.44 No.4 (October 2017)
Research Article View: 1,190 Download: 272

Production of Chitooligosaccharides with Antibacterial Potential via Crude Chitinase Enzymes from Marine Fungi
page: 1224 - 1230

Apiradee Pilantanapak*, Yaowapha Waiprib, Phattharawadee Eadtem and Watanalai Panbangred

Vol.44 No.4 (October 2017)
Research Article View: 1,163 Download: 322

Antibiotics Constituents of Endophytic Bacillus amyloliquefaciens UD25 Extracted from a Medicinal Plant, Memecylon edule Roxb.
page: 788 - 799

Artidtaya Bhoonobtong, Sirirath Sodngam, Sophon Boonlue, Wandee Bunyatratchata and Wiyada Mongkolt

Vol.44 No.3 (July 2017)
Research Article View: 2,340 Download: 1,048

Carotenoid Composition and Antioxidant Activity of Extracts From Tropical Fruits
page: 605 - 616

V ctor M. Moo-Huchin [a], Gustavo A. Gonz lez-Aguilar [b], Mariela Moo-Huchin [c], Elizabeth Ortiz-V zquez [d], Luis Cuevas-Glory [d], Enrique Sauri-Duch [d] and David Betancur-Ancona* [e]

Vol.44 No.2 (April 2017)
Research Article View: 1,183 Download: 1,060

Antioxidant and Antimicrobial Properties of a Brown Alga Scytosiphon lomentaria
page: 595 - 604

Xiao Juan Xu, Yu Zhang, Hong Lin, Chao Kang, Xiao Ting Fu*

Vol.44 No.2 (April 2017)
Research Article View: 1,192 Download: 284

Antioxidant Activities and Stability of Seed Kernel Extracts from Mango (Mangifera indica linn.) Cultivated in Northern Thailand
page: 573 - 583

Nichana Nakpanich, Wantida Chaiyana and Pimporn Leelapornpisid*

Vol.44 No.2 (April 2017)
Research Article View: 1,336 Download: 402

Phytochemical Screening, Phenolic and Flavonoid Contents, Antioxidant and Cytotoxic Activities of Graptophyllum pictum (L.) Griff.
page: 193 - 202

Nadechanok Jiangseubchatveera, Saisunee Liawruangrath, Aphiwat Teerawutgulrag, Dammrong Santiarwor

Vol.44 No.1 (JANUARY 2017)
Research Article View: 1,455 Download: 714

Polyphenol Contents, Antioxidant and Anticancer Activity (MCF-7) of Soybean Products in Thailand
page: 176 - 183

Thidarat Somdee *, Udomsak Mahaweerawat, Jindawan Wibulutai, Namphuang Dungkokruad and Suneerat Yung

Vol.44 No.1 (JANUARY 2017)
Research Article View: 1,285 Download: 1,913

Antibacterial Activity Against Acne Involved Bacteria of Chitosan in a Soluble State and as Nanoparticles
page: 1149 - 1158

Supreedee Sungkharak [a, b], Nicha Supasit [b], Sasiluk Choopan [b] and Suwipa Ungphaiboon*[a, b]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,248 Download: 781

Essential Oil of Solanum spirale Fruits and Its Biological Activities
page: 547 - 555

Sukanya Keawsa-ard [a], Boonsom Liawruangrath* [b], Saisunee Liawruangrath [c], Aphiwat Teerawutgulrag [c] Stephen G. Pyne [d]

Vol.43 No.3 (APRIL 2016)
Research Article View: 1,687 Download: 369

Determination of Antioxidant and Anticancer Activities together with Total Phenol and Flavonoid Contents of Cleidion javanicum Bl. and Bridelia retusa (L.) A. Juss.
page: 535 - 546

Duangsuree Sanseera, Boonsom Liawruangrath *, Stephen G. Pyne and Saisunee Liawruangrath*

Vol.43 No.3 (APRIL 2016)
Research Article View: 1,645 Download: 897

Optimization of the Preparation of Gelatin Hydrolysates with Antioxidative Activity from Lizardfish (Saurida spp.) Scales Gelatin
page: 68 - 79

Sutee Wangtueai [a, b]*, Susanne Siebenhandl-Ehn [c] and Dietmar Haltrich [c]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 1,380 Download: 561

Comparative Studies on Chemical Composition, Phenolic Compounds and Antioxidant Activities of Brown and White Perilla (Perilla frutescens) Seeds
page: 896 - 906

Sikeret Kongkeaw, Siriporn Riebroy and Manat Chaijan

Vol.42 No.4 (OCTOBER 2015)
Research Article View: 1,226 Download: 354

Fatty Acid Composition, Physical Properties, Acute Oral Toxicity and Antioxidant Activity of Crude Lipids from Adipose Tissue of Some Commercialized Freshwater Catfish
page: 626 - 636

Wiwat Wangcharoen, Kriangsak Mengumphan and Doungporn Amornlerdpison

Vol.42 No.3 (JULY 2015)
Research Article View: 1,149 Download: 271

Antimicrobial and antioxidant activity of various solvent extracts of Salsola stenoptera Wagenitz and Petrosimonia nigdeensis Aellen (Chenopodiaceae) plants
page: 156 - 172

Meltem Asan Ozusaglam, Mahmut Erzengin, Derya Onal Darilmaz, Seher Karaman Erkul, Mehtap Tekse

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,347 Download: 252

Phenolics production from a novel substrate palm oil mill effluent by Aspergillus niger IBS-103ZA: Evaluation of fermentation conditions and antioxidant activity
page: 1 - 16

Zulkarnain Mohamed Idris, Parveen Jamal, Md Zahangir Alam, Abdul Haseeb Ansari, A

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,180 Download: 313

In vitro Antioxidant and Antitumor Activities of an Endophytic Fungus Phomopsis liquidambari QH4 from Artemisia annua
page: 992 - 1006

Yixin Qian [a, b], Jichuan Kang*[a, c], Lu Wang [a], Bangxing Lei [a] and Kevin D. Hyde [d, e]

Vol.41 No.5/1 (OCTOBER 2014)
Research Article View: 1,182 Download: 308

Physical Characteristics and Biological Activities of Thai Flower Extracts Loaded in Niosomes
page: 603 - 617

Aranya Manosroi*[a,b], Bang-on Kietthanakorn [b], Charinya Chankhampan [b], Narinthorn Khositsuntiwo

Vol.40 No.4 (OCTOBER 2013)
Research Article View: 1,206 Download: 369

Antibacterial Activity and Chemical Composition of Essential Oil and Various Extracts of Fagraea fragrans Roxb. Flowers
page: 214 - 223

Patcharee Pripdeevech* [a] and Jarupux Saansoomchai [b]

Vol.40 No.2 (APRIL 2013)
Research Article View: 1,327 Download: 543

Antimicrobial and Antioxidant Activities of Methanol, Dichloromethane and Ethyl Acetate Extracts of Scutellaria lindbergii Rech.f.
page: 49 - 59

Bibi Sedigheh Fazly-Bazzaz *[a], Atefeh Arab [b], Seyed Ahmad Emami [c], Javad Asili [c], Mohamad Ha

Vol.40 No.1 (JANUARY 2013)
Research Article View: 1,170 Download: 339

Antioxidant Potential of Pleurotus porrigens Extract and Application in Sunflower Oil during Accelerated Storage
page: 34 - 48

Hip Seng Yim*[a, b], Fook Yee Chye [b], Mei Lian Liow [a], and Chun Wai Ho [a]

Vol.40 No.1 (JANUARY 2013)
Research Article View: 1,181 Download: 365

Nutritional and Physicochemical Qualities of Thua Nao (Thai Traditional Fermented Soybean)
page: 562 - 574

Katekan Dajanta1,2, Ekachai Chukeatirote3,* and Arunee Apichartsrangkoon2

Vol.39 No.4 (OCTOBER 2012)
Research Article View: 1,200 Download: 348

Antioxidant, Antibacterial, Anticancer Activities and Chemical Constituents of the Essential Oil from Mesua ferrea Leaves
page: 455 - 463

Sukanya Keawsa-ard* [a] and Samart Kongtaweelert [b]

Vol.39 No.3 (JULY 2012)
Research Article View: 1,336 Download: 631

Anticancer and Antibacterial Activities of the Isolated Compounds from Solanum spirale Roxb. Leaves
page: 445 - 454

Sukanya Keawsa-ard [a], Surapol Natakankitkul [a], Saisunee Liawruangrath [b], Aphiwat Teerawutgulra

Vol.39 No.3 (JULY 2012)
Research Article View: 1,177 Download: 419

Nostochopsis lobatus Wood em. Geitler (Nostocales), Edible Algae in Northern Thailand
page: 119 - 127

Sorrachat Thiamdao, Manita Motham, Jeeraporn Pekkoh, Lapatrada Mungmai and Yuwadee Peerapornpisal

Vol.39 No.1 (JANUARY 2012)
Research Article View: 1,199 Download: 266

Antibacterial Activity of Bauhinia acuminata L. Seed Protein Extract with Low Hemolytic Activity Against Human Erythrocytes
page: 242 - 251

Karaket Phansri [a], Rakrudee Sarnthima [a], Sompong Thammasirirak [b], Pasakorn Boonchalee [c] and

Vol.38 No.2 (APRIL 2011)
Research Article View: 1,248 Download: 278

Gastroprotective Activity of Padina minor Yamada
page: 92 - 103

Doungporn Amornlerdpison, Yuwadee Peerapornpisal, Tawat Taesotikul, Thidarat Noiraksar, and Duangta

Vol.36 No.1 (JANUARY 2009)
Research Article View: 1,040 Download: 295

Antioxidant and Anti-inflammatory Activities of Brown Marine Alga, Padina minor Yamada
page: 507 - 516

Yuwadee Peerapornpisal, Doungporn Amornlerdpison, Utan Jamjai, Tawat Taesotikul, Yanee Pongpaibul,

Vol.37 No.3 (SEPTEMBER 2010)
Research Article View: 1,237 Download: 384

Chiang Mai Journal of Science

2,437

Q3 1.0

Q2 1.6

7 days

Outline

Figures and Tables