Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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Ultrasonic Extraction, Antioxidant and Anti-glycation Activities of Polysaccharides from Gynura divaricata Leaves

Pawinee Deetae*[a, e], Kwanrudee Tseng [a], Chanathinart Pasuphan [a], Atikorn Panya [b], Pawadee Methacanon [c], Waranya Temthawee [a] and Manop Suphantharika [d]
* Author for corresponding; e-mail address: pawinee_deetae@hotmail.com
Volume: Vol.44 No.1 (JANUARY 2017)
Research Article
DOI:
Received: 27 April 2015, Revised: -, Accepted: 2 December 2015, Published: -

Citation: Deetae[a P., E] , Tseng K., Pasuphan C., Panya A., Methacanon P., et al., Ultrasonic Extraction, Antioxidant and Anti-glycation Activities of Polysaccharides from Gynura divaricata Leaves, Chiang Mai Journal of Science, 2017; 44(1): 144-156.

Abstract

To date, there are numerous studies on Gynura divaricata mainly focused on isolation and biological activities of small chemical compounds such as polyphenols. Only few attentions have been paid on polysaccharides from G. divaricata leaves regarding the extraction, structural characterization and biological functions. Therefore, in this study, ultrasonic extraction, an efficient technique compared to the conventional methods, was applied via response surface methodology to determine the optimum conditions, which maximized the yield of G. divaricata polysaccharides (GDP). Moreover, its physico-chemical and biological properties including in vitro-antioxidant and anti-glycation activities were investigated. The GDP yield under the optimal condition (148 W ultrasonic power, 15 min extraction time and 18.5 ml/g ratio of water to raw material) was found to be 6.45% ± 0.13, which was very close to the value predicted by the mathematical model. The primary characterization using various physico-chemical approaches revealed that GDP is a polysaccharide mainly composed of uronic acid (57.38%) and neutral sugars (40.94%) with molecular weight approximately of 960 kDa. In addition, GDP exhibited remarkable metal chelating antioxidant up to 30% of activity at 60 mg/100 ml and anti-glycation capacity reached 90% at 100 mg/100 ml. Ultrasound-assisted extraction was found to positively affect the GDP yield. The GDP exhibited highly potent antioxidant and anti-glycation properties which could be explored as a novel natural antioxidant and natural glycation inhibitor for food, pharmaceutical and cosmetics industries.

Keywords: polysaccharides, purple velvet, anti-AGE formation, antioxidant, optimization

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Jiradej Manosroi, Charinya Chankhampan, Worapaka Manosroi and Aranya Manosroi*

Vol.44 No.4 (October 2017)
Research Article View: 1,138 Download: 338
Antioxidant, Total Phenolics and Total Flavonoid Content of the Aqueous Extract of Caesalpinia bonducella Seeds
page: 929 - 938

Shruti Shukla* and Archana Mehta

Vol.44 No.3 (July 2017)
Research Article View: 1,114 Download: 474
Total Phenolic and Ascorbic Acid Contents and Antioxidant Activities of Twelve Different Ecotypes of Phyllanthus emblica from Pakistan
page: 904 - 911

Syed Mubashar Sabir*, Rashad Hussain Shah and Asad Hussain Shah

Vol.44 No.3 (July 2017)
Research Article View: 1,018 Download: 516
Innovating Botanical Extraction through Application of Microwaves for the Efficient Extraction of Ursolic Acid from Ocimum sanctum Leaves
page: 869 - 880

Rajendra Mehta, Raj Kumar, Roshni Tandey and Vivekananda Mandal*

Vol.44 No.3 (July 2017)
Research Article View: 984 Download: 304
Carotenoid Composition and Antioxidant Activity of Extracts From Tropical Fruits
page: 605 - 616

V ctor M. Moo-Huchin [a], Gustavo A. Gonz lez-Aguilar [b], Mariela Moo-Huchin [c], Elizabeth Ortiz-V zquez [d], Luis Cuevas-Glory [d], Enrique Sauri-Duch [d] and David Betancur-Ancona* [e]

Vol.44 No.2 (April 2017)
Research Article View: 1,029 Download: 1,054
Antioxidant and Antimicrobial Properties of a Brown Alga Scytosiphon lomentaria
page: 595 - 604

Xiao Juan Xu, Yu Zhang, Hong Lin, Chao Kang, Xiao Ting Fu*

Vol.44 No.2 (April 2017)
Research Article View: 1,035 Download: 277
Antioxidant Activities and Stability of Seed Kernel Extracts from Mango (Mangifera indica linn.) Cultivated in Northern Thailand
page: 573 - 583

Nichana Nakpanich, Wantida Chaiyana and Pimporn Leelapornpisid*

Vol.44 No.2 (April 2017)
Research Article View: 1,123 Download: 401
Chemical Profiles and Antimicrobial Activities of Thai Propolis Collected from Apis mellifera
page: 438 - 448

Sirikarn Sanpa [a,b], Milena Popova [c], Tawee Tunkasiri [d], Sukum Eitssayeam [d], Vassya Bankova [

Vol.44 No.2 (April 2017)
Research Article View: 1,858 Download: 473
Phytochemical Screening, Phenolic and Flavonoid Contents, Antioxidant and Cytotoxic Activities of Graptophyllum pictum (L.) Griff.
page: 193 - 202

Nadechanok Jiangseubchatveera, Saisunee Liawruangrath, Aphiwat Teerawutgulrag, Dammrong Santiarwor

Vol.44 No.1 (JANUARY 2017)
Research Article View: 1,240 Download: 686
Polyphenol Contents, Antioxidant and Anticancer Activity (MCF-7) of Soybean Products in Thailand
page: 176 - 183

Thidarat Somdee *, Udomsak Mahaweerawat, Jindawan Wibulutai, Namphuang Dungkokruad and Suneerat Yung

Vol.44 No.1 (JANUARY 2017)
Research Article View: 1,118 Download: 1,909
Optimization of Xylitol Production by Candida tropicalis A26
page: 50 - 58

Wanlapa Lorliam [a], Ancharida Akaracharanya [b], Sukhumaporn Krajangsang [c], Vasana Tolieng [d] a

Vol.44 No.1 (JANUARY 2017)
Research Article View: 1,090 Download: 254
Phenolic Profile, Fatty Acid and Essential Oil Composition Analysis and Antioxidant, Antialzheimer and Antibacterial Activities of Verbascum flavidum Extracts
page: 1090 - 1101

Mehmet Boğa*[a], Abdulselam Ertaş [b], Nesrin Haşimi [c], Serpil Demirci [d], Mustafa Abdullah Yılmaz [e], Hamdi Temel [f] and Ufuk Kolak [g]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,059 Download: 378
Utilization of Non-rubber Skim Latex for Poly(L-lactide)-degrading Enzyme Production by Actinomadura keratinilytica Strain T16-1
page: 1016 - 1026

Sabadee Worrathampitak [a], Shinji Tokuyama [b], Vichien Kitpreechavanich [c] and Sukhumaporn Sukkhum* [a]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,052 Download: 368
Antioxidant Compounds and Activities in Selected Fresh and Blanched Vegetables from Northeastern Thailand
page: 834 - 844

Thidarat Somdee*[a], Udomsak Mahaweerawat [a], Methin Phadungkit [b] and Suneerat Yangyuen [a]

Vol.43 No.4 (JULY 2016)
Research Article View: 998 Download: 291
Synthesis, Characterization and Antioxidant Activity of 2-Halobenzoyl Thiourea bearing α- and β-Alanine
page: 578 - 584

Nurziana Ngah*, Nor Azanita Mohamed, and Deny Susanti Darnis

Vol.43 No.3 (APRIL 2016)
Research Article View: 940 Download: 318
Determination of Antioxidant and Anticancer Activities together with Total Phenol and Flavonoid Contents of Cleidion javanicum Bl. and Bridelia retusa (L.) A. Juss.
page: 535 - 546

Duangsuree Sanseera, Boonsom Liawruangrath *, Stephen G. Pyne and Saisunee Liawruangrath*

Vol.43 No.3 (APRIL 2016)
Research Article View: 1,514 Download: 867
Phytochemical Constituents with Antimicrobial and Antioxidant Activities from Xylia xylocarpa (Roxb.) Taub. Sawdust Extracts
page: 11 - 21

Pattarawadee Sumthong Nakmee* [a], Soontree Khuntong [a] and Nitra Nuengchamnong [b]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 1,071 Download: 424
Optimization of the Preparation of Gelatin Hydrolysates with Antioxidative Activity from Lizardfish (Saurida spp.) Scales Gelatin
page: 68 - 79

Sutee Wangtueai [a, b]*, Susanne Siebenhandl-Ehn [c] and Dietmar Haltrich [c]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 1,186 Download: 559
Antioxidant Capacity and Phenolic Content Evaluation on Peanut Skins from 3 Peanut Types
page: 123 - 137

Pornnapa Chuenchom, Prasan Swatsitang, Thanaset Senawong, Sanun Jogloy

Vol.43 No.1 (JANUARY 2016)
Research Article View: 1,107 Download: 594
Insights Into The Causes and Anti-hyperglycemic Effects Of Punica granatum Rind in Alloxan-induced Diabetic Rats
page: 112 - 122

Sushil Kumar Middha [a,c], Talambedu Usha [b]* and Veena Pande [a]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 928 Download: 406
Ameliorating Reactive Oxygen Species-Induced in vitro Lipid Peroxidation in Liver, Carbohydrate and DNA Damage by Dendrocalamus hamiltonii Different Leaf Extracts
page: 80 - 88

Arvind Kumar Goyal [a,b], Sushil Kumar Middha [c], Talambedu Usha [c] and Arnab Sen*[a]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 993 Download: 245
Fatty Acid, Essential Oil and Phenolic Compositions of Alcea pallida and Alcea apterocarpa with Antioxidant, Anticholinesterase and Antimicrobial Activities
page: 89 - 99

Abdulselam Ertas*[a,b], Mehmet Boga [b,c], Isil Gazioglu [d], Yeter Yesil [e] , Nesrin Hasimi [f], Cumali Ozaslan [g], Hasibe Yilmaz[h], Muammer Kaplan[j]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 1,040 Download: 379
Analysis of Chitin, Chitosan, and Optimization for Carotenoids Extraction Yield with Rice Bran Oil from Thai Fairy Shrimp
page: 918 - 929

Tanyarat Poothawan and Khomsorn Lomthaisong

Vol.42 No.4 (OCTOBER 2015)
Research Article View: 999 Download: 408
Comparative Studies on Chemical Composition, Phenolic Compounds and Antioxidant Activities of Brown and White Perilla (Perilla frutescens) Seeds
page: 896 - 906

Sikeret Kongkeaw, Siriporn Riebroy and Manat Chaijan

Vol.42 No.4 (OCTOBER 2015)
Research Article View: 1,074 Download: 349
HPTLC Analysis, Antioxidant, Anti-Inflammatory and Xanthine Oxidase Inhibitory Activity of Plumbago zeylanica L
page: 886 - 895

Shivraj Hariram Nile, Umesh Bapurao Patil and Se Won Park

Vol.42 No.4 (OCTOBER 2015)
Research Article View: 1,043 Download: 514
Medium Optimization for b-Galactosidase Production by a Thermotolerant Yeast
page: 840 - 849

Sirinda Am-aiam and Chartchai Khanongnuch

Vol.42 No.4 (OCTOBER 2015)
Research Article View: 1,375 Download: 296
Prediction of Antioxidant Capacity of Thai Vegetable Extracts by Infrared Spectroscopy
page: 657 - 668

Trakul Prommajak, Sang Moo Kim, Cheol-Ho Pan, Sang Min Kim, Suthat Surawang, Nithiya

Vol.42 No.3 (JULY 2015)
Research Article View: 1,071 Download: 334
Determination of the Effects of Adding Milk and Sugar on the Antioxidant Capacity of Oolong Tea by Chemical and Cell Culture-Based Antioxidant Assays
page: 699 - 711

Angkana Wipatanawin, Sudarat Phongsawanit, Thadthanan Maneeratprasert, Sittiwat Lertsiri

Vol.42 No.3 (JULY 2015)
Research Article View: 1,152 Download: 377
Improvement of production and stability of silk degumming protease by Bacillus sp. C4 SS-2013
page: 599 - 613

Nisa Romsomsa, Patoomporn Chim-anage, Sarote Sirisansaneeyakul

Vol.42 No.3 (JULY 2015)
Research Article View: 1,371 Download: 290
Fatty Acid Composition, Physical Properties, Acute Oral Toxicity and Antioxidant Activity of Crude Lipids from Adipose Tissue of Some Commercialized Freshwater Catfish
page: 626 - 636

Wiwat Wangcharoen, Kriangsak Mengumphan and Doungporn Amornlerdpison

Vol.42 No.3 (JULY 2015)
Research Article View: 987 Download: 265
Phenolics production from a novel substrate palm oil mill effluent by Aspergillus niger IBS-103ZA: Evaluation of fermentation conditions and antioxidant activity
page: 1 - 16

Zulkarnain Mohamed Idris, Parveen Jamal, Md Zahangir Alam, Abdul Haseeb Ansari, A

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,046 Download: 309
Statistical Optimization for the Production of D-Mannose Isomerase by Saccharothrix sp. CMU-K747 Using Response Surface Methodology
page: 52 - 61

ApiradeeSiangsuepchart [a] and SaisamornLumyong*[a, b]

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,020 Download: 315
In vitro Antioxidant and Antitumor Activities of an Endophytic Fungus Phomopsis liquidambari QH4 from Artemisia annua
page: 992 - 1006

Yixin Qian [a, b], Jichuan Kang*[a, c], Lu Wang [a], Bangxing Lei [a] and Kevin D. Hyde [d, e]

Vol.41 No.5/1 (OCTOBER 2014)
Research Article View: 1,027 Download: 305
Minimal Changes in the Right Hand Side Data for Correcting Linear Infeasibility Arising in Intensity-Modulated Radiation Therapy with the Generalized Newton Method
page: 1439 - 1443

Saeed Ketabchi [a], Hossein Moosaei [b] and Ying Xiao [c]

Vol.41 No.5/2 (OCTOBER 2014)
Research Article View: 903 Download: 247
Gastroprotective Effects and Antioxidant Activities of Paederia pilifera Hook.f. Root Extract
page: 1121 - 1131

Kantarat Jirakittirat, Supap Saenphet and Kanokporn Saenphet*

Vol.41 No.5/1 (OCTOBER 2014)
Research Article View: 894 Download: 269
Optimization of Solid-state Fermentation for Fruiting Body Growth and Cordecepin Production by Cordyceps militaris
page: 858 - 872

Ting-chi Wen, Cuang-rong Li, Ji-chuan Kang*,Chao Kang and Kevin D. Hyde

Vol.41 No.4 (SPECIAL ISSUE 1)
Research Article View: 1,230 Download: 583
Phenolic acids content and antioxidant capacity of fruit extracts from Thailand
page: 636 - 642

Nattaya Ongphimai [a], Supathra Lilitchan*[a], Kornkanok Aryusuk [b], Akkarach Bumrungpert [a], et a

Vol.40 No.4 (OCTOBER 2013)
Research Article View: 1,052 Download: 366
Physical Characteristics and Biological Activities of Thai Flower Extracts Loaded in Niosomes
page: 603 - 617

Aranya Manosroi*[a,b], Bang-on Kietthanakorn [b], Charinya Chankhampan [b], Narinthorn Khositsuntiwo

Vol.40 No.4 (OCTOBER 2013)
Research Article View: 1,032 Download: 361
Role of Quercetin and L-Arginine in Alleviating Zinc Oxide Nanoparticle Hepatotoxicity in Rats
page: 577 - 592

Nayira A. Abdel Baky [a]*, L.M. Faddah [a] , Nouf M. Al-Rasheed [a], Nawal M. Al-Rasheed [a] , and W

Vol.40 No.4 (OCTOBER 2013)
Research Article View: 1,003 Download: 321
The Potential of Electrospun Poly(L-lactic Acid) Fiber Mats Containing a Crude Garcinia Dulcis Extract for Use as Wound Dressings
page: 517 - 533

Orawan Suwantong *[a], Porntipa Pankongadisak [a], Suwanna Deachathai [a] and Pitt Supaphol [b]

Vol.40 No.3 (JULY 2013)
Research Article View: 987 Download: 312
Development of Sequential Injection Analysis Using Peristaltic Pump and Electrochemical Detection for Antioxidant Capacity Test by ABTS Assay
page: 224 - 231

Jakkrapun Kongkedsuk [a], Apisake Hongwitayakorn [a], Wannarekha Bootnapang [b], Nisachon Boonto [b

Vol.40 No.2 (APRIL 2013)
Research Article View: 1,429 Download: 338
Antimicrobial and Antioxidant Activities of Methanol, Dichloromethane and Ethyl Acetate Extracts of Scutellaria lindbergii Rech.f.
page: 49 - 59

Bibi Sedigheh Fazly-Bazzaz *[a], Atefeh Arab [b], Seyed Ahmad Emami [c], Javad Asili [c], Mohamad Ha

Vol.40 No.1 (JANUARY 2013)
Research Article View: 1,007 Download: 333
Antioxidant Potential of Pleurotus porrigens Extract and Application in Sunflower Oil during Accelerated Storage
page: 34 - 48

Hip Seng Yim*[a, b], Fook Yee Chye [b], Mei Lian Liow [a], and Chun Wai Ho [a]

Vol.40 No.1 (JANUARY 2013)
Research Article View: 1,034 Download: 359
Antioxidant and Sensory Properties of Thai Herbal Teas with emphasis on Thunbergia laurifolia Lindl.
page: 599 - 609

Eric W.C. Chan*, Suit Ying Eng, Yuen Ping Tan, Zhiew Cheng Wong, Phui Yan Lye, and Lea Ngar Tan

Vol.39 No.4 (OCTOBER 2012)
Research Article View: 1,208 Download: 353
Nutritional and Physicochemical Qualities of Thua Nao (Thai Traditional Fermented Soybean)
page: 562 - 574

Katekan Dajanta1,2, Ekachai Chukeatirote3,* and Arunee Apichartsrangkoon2

Vol.39 No.4 (OCTOBER 2012)
Research Article View: 971 Download: 342
Antioxidant, Antibacterial, Anticancer Activities and Chemical Constituents of the Essential Oil from Mesua ferrea Leaves
page: 455 - 463

Sukanya Keawsa-ard* [a] and Samart Kongtaweelert [b]

Vol.39 No.3 (JULY 2012)
Research Article View: 1,074 Download: 626
In vitro INHIBITION OF LIPID PEROXIDATION AND THE ANTIOXIDANT SYSTEM OF ENDOPHYTIC FUNGI FROM THAI MEDICINAL PLANTS
page: 429 - 444

Teerayut Theantana [a,b], Duangta Kanjanapothi [c], Saisamorn Lumyong* [b]

Vol.39 No.3 (JULY 2012)
Research Article View: 1,019 Download: 294
Comparative Analysis of Aqueous Extracts of Amaranth and Coriander in Scavenging Free Radical Activity and Protection of DNA against Oxidative Damage
page: 560 - 571

Asmita Dutta and Mukesh Singh

Vol.38 No.4 (OCTOBER 2011)
Research Article View: 996 Download: 404
Optimization of Media Components and Operating Conditions for Exogenous Production of Fungal L-asparaginase
page: 270 - 279

Gurunathan Baskar [a], and Sahadevan Renganathan*[b]

Vol.38 No.2 (APRIL 2011)
Research Article View: 944 Download: 231
Optimization of Micro Metal Injection Molding with Multiple Performance Characteristics using Grey Relational Grade
page: 231 - 241

Mohd H.I. Ibrahim*[a,b], Norhamidi Muhamad [b], Abu B. Sulong [b], Khairur R. Jamaludin [c], Nor H.

Vol.38 No.2 (APRIL 2011)
Research Article View: 956 Download: 248
A Comparative Analysis of Conjugate Gradient Algorithms & PSO Based Neural Network Approaches for Reusability Evaluation of Procedure Based Software Systems
page: 123 - 135

Parvinder S. Sandhu*[a], Shalini Chhabra [b]

Vol.38 (SPECIAL ISSUE 2011)
Research Article View: 982 Download: 217
Antioxidant Capacity of Broccoli Seeds Grown in Thailand
page: 117 - 122

Sarunya Chuanphongpanich* [a], Maitree Suttajit [b], Sukon Phanichphant [c Duang Buddhasukh [c]

Vol.33 No.1 (JANUARY 2006)
Research Article View: 1,003 Download: 367
Extraction of Phenolic Compounds from Fruits of Bitter Melon (Momordica charantia ) with Subcritical Water Extraction and Antioxidant Activities of These Extracts
page: 123 - 130

Parichat Budrat and Artiwan Shotipruk

Vol.35 No.1 (JANUARY 2008)
Research Article View: 1,059 Download: 677
Red Yeast Rice Prepared from Thai Glutinous Rice and the Antioxidant Activities
page: 42 - 49

Em-on Chairote, Griangsak Chairote, and Saisamorn Lumyong

Vol.36 No.1 (JANUARY 2009)
Research Article View: 1,171 Download: 374
Screening of Antioxidants from Some Thai Vegetables and Herbs
page: 1 - 5

Anchana Chanwitheesuk, Nuansri Rakariyatham*, Aphiwat Teerawutgulrag and Teerapol Wongchanapiboon

Vol.29 No.1 (APRIL 2002)
Opinion View: 1,161 Download: 367
Sensitivity Enhancement of Differential Pulse Polarographic Technique by Simplex Optimization Method
page: 71 - 81

Buabarn Poonperm, Waret Veerasai* and Prapin Wilairat

Vol.27 No.2 (DECEMBER 2000)
Opinion View: 931 Download: 250
Antioxidant and Anti-inflammatory Activities of Brown Marine Alga, Padina minor Yamada
page: 507 - 516

Yuwadee Peerapornpisal, Doungporn Amornlerdpison, Utan Jamjai, Tawat Taesotikul, Yanee Pongpaibul,

Vol.37 No.3 (SEPTEMBER 2010)
Research Article View: 1,065 Download: 375
In vitro Antioxidant Activity of Vitex negundo L. Leaf Extracts
page: 489 - 497

Raghavendra H. Lakshmanashetty, Vijayananda B. Nagaraj, Madhumathi G. Hiremath and Vadlapudi Kumar

Vol.37 No.3 (SEPTEMBER 2010)
Research Article View: 1,559 Download: 1,186
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