Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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Phenolic Profile, Fatty Acid and Essential Oil Composition Analysis and Antioxidant, Antialzheimer and Antibacterial Activities of Verbascum flavidum Extracts

Mehmet Boğa*[a], Abdulselam Ertaş [b], Nesrin Haşimi [c], Serpil Demirci [d], Mustafa Abdullah Yılmaz [e], Hamdi Temel [f] and Ufuk Kolak [g]
* Author for corresponding; e-mail address: mehmetboga1980@gmail.com; mehmet.boga@dicle.edu.tr
Volume: Vol.43 No.5 (OCTOBER 2016)
Research Article
DOI:
Received: 12 December 2014, Revised: -, Accepted: 12 April 2015, Published: -

Citation: Boğa M., Ertaş A., Haşimi N., Demirci S., Yılmaz M.A., Temel H., et al., Phenolic Profile, Fatty Acid and Essential Oil Composition Analysis and Antioxidant, Antialzheimer and Antibacterial Activities of Verbascum flavidum Extracts, Chiang Mai Journal of Science, 2016; 43(5): 1090-1101.

Abstract

This paper is the first report about phytochemical analysis with LC-MS/MS and biological activities of Verbascum flavidum (Boiss.) Freyn & Bornm. Rutin (1077.09±77.47 µg analyte/g extract) and chlorogenic acid (1012.42±97.18 µg analyte/g extract) were determined the most abundant phenolics in V. flavidum. The main components of fatty acid were found to be palmitic (30.3%) and oleic acids (17.9%); while the major essential oils were determined as arachidic acid (16.4%) and α-selinene (8.9%) by GC-MS analysis. In DPPH free radical scavenging activity the methanol extract showed higher activity than BHT with 72.62% inhibition at 100 µg/mL concentration. The methanol and water extracts of V. flavidum showed 86.01 % and 87.39 % inhibition in ABTS cation radical scavenging assay at 100 μg/mL concentration, respectively. The methanol extract showed moderate activity against E. coli with 250±0.3 MIC value and with 15 mm inhibition zone diameter at 30 mg/mL concentration.

Keywords: Verbascum flavidum, antioxidant, anticholinesterase, antimicrobial, LC-MS/MS, GC-MS

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