Chiang Mai Journal of Science

Identificationof Bioactive Moleculesfrom Thai Centipede, Scolopendra subspinipes dehaani, Venom

Paper Type	Contributed Paper
Title	Identificationof Bioactive Moleculesfrom Thai Centipede, Scolopendra subspinipes dehaani, Venom
Author	Muchalin Meunchan, Nunthawun Uawonggul, Praroonkorn Incamnoi, Sophida Sukprasert, Prapenpuksiri Rungsa, Theerasak Somdee, Sittiruk Roytrakul, Sompong Thammasirirak and Sakda Daduang*
Email	sakdad@kku.ac.th
Abstract:  Centipede venoms are rich sources of bioactive molecules with various actions. These bioactive molecules obtained from Thai centipede, S. subspinipes dehaani, venom have been investigated by proteomic approach and bioactivity assay. Protein profile analysis by SDS-PAGE technique showed 7 major bands with a molecular weight ranging under 66 kDa.  After resolved in 2D-PAGE gel, numerous spots of proteins were found in centipede venom. The trains of major spots were observed at 22 and 45 kDa. The 10 major protein spots were analyzed byliquid chromatography coupled to tandem mass spectrometry(LC-MS/MS). The presence of protease and phospholipase activities in centipede venom was tested by zymographic method andon activity indicator plate (PCY plate), respectively.These molecules were classified into 4 groups including cysteine-rich venom protein, protease, hyaluronidase and phospholipase.
Start & End Page	1 - 10
Received Date	2013-03-10
Revised Date
Accepted Date	2014-12-13
Full Text	Download
Keyword	bioactive molecules, Scolopendrasubspinipesdehaani, 2D-PAGE, LC-MS/MS
Volume	Vol.43 No.1 (JANUARY 2016)
DOI
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