Chiang Mai Journal of Science

Comparative analysis of protein profiles of toxic and non-toxic Jatropha curcas latex

Paper Type	Contributed Paper
Title	Comparative analysis of protein profiles of toxic and non-toxic Jatropha curcas latex
Author	Lucsame Gurnecka, Eleni Gentekakia, Janthima Jaresitthikunchaib, Teerawit Waratrujiwonga, Sittiruk R
Email	siam@mfu.ac.th
Abstract: Latex proteins have been previously characterized in various plants including their indispensable roles. However, latex protein profiles are not well defined in Jatropha, neither of toxic nor non-toxic varieties. To compare the protein profiles between toxic and non-toxic latex we employed a proteomic approach using one-dimensional gel electrophoresis in conjunction with LC-MS/MS. Proteomic analysis of Jatropha latex revealed 421 proteins, of which 368 were shared between the two varieties. Functional classification showed that the most abundant proteins in both toxic and non-toxic Jatropha latex were related to cellular and metabolic processes, while some were associated with immune system processes.  Furthermore, some unique proteins were classified as defense-related highlighting the special role of latex in the defense mechanism of Jatropha. Finally, we identified proteins belonging to the phenylpropanoid, carotenoid and flavonoid biosynthesis pathways. To the extent of our knowledge, this study provides the first comprehensive proteomic data for Jatropha latex.
Start & End Page	1379 - 1391
Received Date	2016-07-15
Revised Date
Accepted Date	2016-09-22
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Keyword	Jatropha curcas, latex, LC-MS/MS, one dimensional gel electrophoresis, proteomic analysis
Volume	Vol.44 No.4 (October 2017)
DOI
Citation	Gurnecka L., Gentekakia E., Jaresitthikunchaib J., Waratrujiwonga T. and R S., Comparative analysis of protein profiles of toxic and non-toxic Jatropha curcas latex, Chiang Mai J. Sci., 2017; 44(4): 1379-1391.
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