Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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A Rapid Process for Purification of Chitinase from the Latex of Carica papaya

Sarote Nitsawang [a] and Pawinee Kanasawud* [b]
* Author for corresponding; e-mail address: scipknsw@chiangmai.ac.th
Volume: Vol.33 No.2 (MAY 2006)
Short Communication
DOI:
Received: 25 July 2005, Revised: -, Accepted: 30 November 2005, Published: -

Citation: Nitsawang S. and Kanasawud P., A Rapid Process for Purification of Chitinase from the Latex of Carica papaya, Chiang Mai Journal of Science, 2006; 33(2): 237-242.

Abstract

A rapid process for purification of chitinase from the latex of Carica papaya involves an aqueous two-phase extraction and salt fractionation. The whole latex, including the insoluble material, was extracted by 5% PEG-12% (NH4)2SO4 to remove papain. Chitinase was purified from the salt-rich bottom phase by precipitation with 11% ammonium sulfate and 20% sodium chloride, respectively: 2.4 mg enzyme/g of wet latex and 4.2 folds of purification were obtained. The purified enzyme was homogeneous on cathodic gel electrophoresis and its Mw of 26540 daltons was determined by ESI-MS. It can be classified as class II chitinase by its N-terminal amino acid sequence, GIEKII.

Keywords: Carica papaya latex, chitinase, purification

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