Chiang Mai Journal of Science

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A rapid process for purification of chitinase from the latex of Carica papaya involves an aqueous two-phase extraction and salt fractionation. The whole latex, including the insoluble material, was extracted by 5% PEG-12% (NH₄)₂SO₄ to remove papain. Chitinase was purified from the salt-rich bottom phase by precipitation with 11% ammonium sulfate and 20% sodium chloride, respectively: 2.4 mg enzyme/g of wet latex and 4.2 folds of purification were obtained. The purified enzyme was homogeneous on cathodic gel electrophoresis and its Mw of 26540 daltons was determined by ESI-MS. It can be classified as class II chitinase by its N-terminal amino acid sequence, GIEKII.

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