Submit your paper 
Download Template JOURNAL DETAIL
A Rapid Process for Purification of Chitinase from the Latex of Carica papaya
Paper Type  |
Short Communication |
Title |
A Rapid Process for Purification of Chitinase from the Latex of Carica papaya |
Author |
Sarote Nitsawang [a] and Pawinee Kanasawud* [b] |
Email |
scipknsw@chiangmai.ac.th |
|
Abstract: A rapid process for purification of chitinase from the latex of Carica papaya involves an aqueous two-phase extraction and salt fractionation. The whole latex, including the insoluble material, was extracted by 5% PEG-12% (NH4)2SO4 to remove papain. Chitinase was purified from the salt-rich bottom phase by precipitation with 11% ammonium sulfate and 20% sodium chloride, respectively: 2.4 mg enzyme/g of wet latex and 4.2 folds of purification were obtained. The purified enzyme was homogeneous on cathodic gel electrophoresis and its Mw of 26540 daltons was determined by ESI-MS. It can be classified as class II chitinase by its N-terminal amino acid sequence, GIEKII.
|
|
Start & End Page |
237 - 242 |
Received Date |
2005-07-25 |
Revised Date |
|
Accepted Date |
2005-11-30 |
Full Text |
Download |
Keyword |
Carica papaya latex, chitinase, purification |
Volume |
Vol.33 No.2 (MAY 2006) |
DOI |
|
Citation |
[a] S.N. and [b] P.K., A Rapid Process for Purification of Chitinase from the Latex of Carica papaya, Chiang Mai Journal of Science, 2006; 33(2): 237-242. |
SDGs |
|
| View:602 Download:185 | |
RELATED ARTICLE
Purification and Characterization of an Extracellular Chitinase from Bacillus thuringiensis R 176 using Solid State Fermentation with Shrimp Shells Waste
page: 495 - 509
Author:Mathurot Chaiharn, Abhinya Pikomol and Saisamorn Lumyoung
Vol.46 No.3 (May 2019) View: 670 Download:292
page: 495 - 509
Author:Mathurot Chaiharn, Abhinya Pikomol and Saisamorn Lumyoung
Vol.46 No.3 (May 2019) View: 670 Download:292
Optimization, Purification and Characterization of b-xylanase by a Novel Thermotolerant Strain of Microbispora siamensis, DMKUA 245T
page: 2267 - 2282
Author:Antika Boondaeng, Sukhumaporn Krajangsang, Chanaporn Trakunjae, Thanasak Lomthong, Shinji Tokuyama and Vichien Kitpreechavanich
Vol.45 No.6 (September 2018) View: 660 Download:360
page: 2267 - 2282
Author:Antika Boondaeng, Sukhumaporn Krajangsang, Chanaporn Trakunjae, Thanasak Lomthong, Shinji Tokuyama and Vichien Kitpreechavanich
Vol.45 No.6 (September 2018) View: 660 Download:360
Response Surface Methodology Based Optimized Purification of the Residual Glycerol from Biodiesel Production Process
page: 1570 - 1582
Author:Muhammad Danish*, Muhammad Waseem Mumtaz [a], Mahpara Fakhar [a] and Umer Rashid*
Vol.44 No.4 (October 2017) View: 822 Download:511
page: 1570 - 1582
Author:Muhammad Danish*, Muhammad Waseem Mumtaz [a], Mahpara Fakhar [a] and Umer Rashid*
Vol.44 No.4 (October 2017) View: 822 Download:511
Production and Characterization of Bacteriocin-producing Bacteria Capable of Inhibition of Streptococcus dysgalactiae
page: 366 - 374
Author:Nalin Wongkattiya [a], Ian H. Fraser [b], Piyanuch Niamsup [a], Phanchana Sanguansermsri [c] and Don
Vol.44 No.2 (April 2017) View: 683 Download:347
page: 366 - 374
Author:Nalin Wongkattiya [a], Ian H. Fraser [b], Piyanuch Niamsup [a], Phanchana Sanguansermsri [c] and Don
Vol.44 No.2 (April 2017) View: 683 Download:347
Partial Purification and Comparison of Precipitation Techniques of Pyruvate Decarboxylase Enzyme
page: 184 - 192
Author:Julaluk Tangtua, Charin Techapun, Ronachai Pratanaphon, Ampin Kuntiya, Vorapat Sanguanchaipaiwong, T
Vol.44 No.1 (JANUARY 2017) View: 714 Download:265
page: 184 - 192
Author:Julaluk Tangtua, Charin Techapun, Ronachai Pratanaphon, Ampin Kuntiya, Vorapat Sanguanchaipaiwong, T
Vol.44 No.1 (JANUARY 2017) View: 714 Download:265
Biochemical Characterization of Two Acid Phosphatases Purified from Breadfruit (Artocarpus communis) Seeds
page: 1100 - 1112
Author:Hubert Kouassi Konan*, Desire Yapi Assoi Yapi, Clement Yao Yue Bi, Thierry Fankroma Martial Kone , Parfait Eugene Jean N’guessan Kouadio and Lucien Patrice Kouame
Vol.43 No.5 (OCTOBER 2016) View: 638 Download:806
page: 1100 - 1112
Author:Hubert Kouassi Konan*, Desire Yapi Assoi Yapi, Clement Yao Yue Bi, Thierry Fankroma Martial Kone , Parfait Eugene Jean N’guessan Kouadio and Lucien Patrice Kouame
Vol.43 No.5 (OCTOBER 2016) View: 638 Download:806
Purification and Evaluation of Bioactive Fractions for Anti-Cancer Potentials from the Flowers and Leaves of Nyctanthes arbor-tristis L.
page: 100 - 111
Author:Bibechana Timsina[a] and Varalakshmi Kilingar Nadumane*[a]
Vol.43 No.1 (JANUARY 2016) View: 1,141 Download:360
page: 100 - 111
Author:Bibechana Timsina[a] and Varalakshmi Kilingar Nadumane*[a]
Vol.43 No.1 (JANUARY 2016) View: 1,141 Download:360
Antibacterial Agents from Trichoderma harzianum strain T9 Against Pathogenic Bacteria
page: 304 - 316
Author:Weeraya Phupiewkham, Pisan Sirithorn, Weerasak Saksirirat, Sompong Thammasirirak
Vol.42 No.2 (APRIL 2015) View: 1,155 Download:247
page: 304 - 316
Author:Weeraya Phupiewkham, Pisan Sirithorn, Weerasak Saksirirat, Sompong Thammasirirak
Vol.42 No.2 (APRIL 2015) View: 1,155 Download:247
Purification and Characterization of Fungal Laccase from Mycena purpureofusca
page: 151 - 160
Author:Shujing Sun1, Yonghui Zhang1, Youxiong Que2, Bixian Liu1, Kaihui Hu1, and Liping Xu2*
Vol.40 No.2 (APRIL 2013) View: 666 Download:189
page: 151 - 160
Author:Shujing Sun1, Yonghui Zhang1, Youxiong Que2, Bixian Liu1, Kaihui Hu1, and Liping Xu2*
Vol.40 No.2 (APRIL 2013) View: 666 Download:189
Cloning and Expression of Chitinase Gene Isolated
from Insect Pathogenic Fungi, Beauveria bassiana in
Escherichia coli
page: 347 - 355
Author:Khemika Songjang*[a], Tawee Donchai [a], Paranya Chaiyawat [a] and Christopher R. Meyer [b]
Vol.33 No.3 (SEPTEMBER 2006) View: 1,117 Download:198
page: 347 - 355
Author:Khemika Songjang*[a], Tawee Donchai [a], Paranya Chaiyawat [a] and Christopher R. Meyer [b]
Vol.33 No.3 (SEPTEMBER 2006) View: 1,117 Download:198
Simple Purification of Indirubin from Indigofera
tinctoria Linn. and Inhibitory Effect on MCF-7 Human
Breast Cancer Cells
page: 329 - 337
Author:Paitoon Aobchey , Supachok Sinchaikul , Suree Phutrakul and Shui-Tein Chen
Vol.34 No.3 (SEPTEMBER 2007) View: 698 Download:254
page: 329 - 337
Author:Paitoon Aobchey , Supachok Sinchaikul , Suree Phutrakul and Shui-Tein Chen
Vol.34 No.3 (SEPTEMBER 2007) View: 698 Download:254
Proteomic Study of Recombinant Escherichia coli
Expressing Beauveria bassiana Chitinase Gene
page: 324 - 330
Author:Khemika Lomthaisong, Kanchanit Boonmaleerat and Aphinya Wongpia
Vol.35 No.2 (MAY 2008) View: 672 Download:173
page: 324 - 330
Author:Khemika Lomthaisong, Kanchanit Boonmaleerat and Aphinya Wongpia
Vol.35 No.2 (MAY 2008) View: 672 Download:173
Optimising the Purification of Terpyridine-Cytochrome
c Bioconjugates
page: 236 - 246
Author:Joshua R. Peterson, and Pall Thordarson
Vol.36 No.2 (MAY 2009) View: 677 Download:214
page: 236 - 246
Author:Joshua R. Peterson, and Pall Thordarson
Vol.36 No.2 (MAY 2009) View: 677 Download:214
Copyrights © Since 2021 All Rights Reserved by Chiang Mai Journal of Science