Journal Volumes


Visitors
ALL : 1,004,973
TODAY : 2,955
ONLINE : 19



















  JOURNAL DETAIL



Antibacterial Agents from Trichoderma harzianum strain T9 Against Pathogenic Bacteria


Paper Type 
Contributed Paper
Title 
Antibacterial Agents from Trichoderma harzianum strain T9 Against Pathogenic Bacteria
Author 
Weeraya Phupiewkham, Pisan Sirithorn, Weerasak Saksirirat, Sompong Thammasirirak
Email 
somkly@kku.ac.th
Abstract:

        Trichoderma harzianum is an important fungi that well known for their ability to produce a wide range of secondary metabolite, antibiotic substance and cell-wall degrading enzyme (CWDE) against phytopathogenic fungi. However, information on the antimicrobial agent in T. harzianum isolate T9 has not been reported. Hence, this research is the first report about antibiotic activity of Trichoderma metabolites against human pathogenic bacteria and enterobacteria. Data for antimicrobial activity were measured at 3 (TS3), 6 (TS6), 9 (TS9), 12 (TS12) and 15 (TS15) days after culture. All culture supernatants from each culture day showed broad-spectrum activity in inhibiting growth of human pathogens and enterobacteria. Especially, inhibiting the growth of Salmonella typhi ATCC5784, this is a human-specific pathogen. Moreover, it was stable to thermal and pronase digestion. Therefore, these culture supernatants were further characterized by thin layer chromatography (TLC), high-performance liquid chromatography (HPLC) and proteomics analysis. The TLC fractions activity shows that their activity was loss when compared with the crude compound. Due to the higher antimicrobial activity of TS3 and TS12, their fractions were chosen for further purification by C18 Reversed-phase HPLC. The results showed that antibacterial activity against Staphylococcus aureus  ATCC25923 of these samples was found only one peak of TS3 (F1) and two peaks of TS12 (F1 and F2). Additionally, proteomics analysis shows that the secreted proteins in log and early late log phase appeared to be a proteases enzyme. From our results, it is likely that T. harzianum isolate T9 against human pathogenic bacteria and enterobacteria with multiple mechanisms including release of antimicrobial agent and CWDE.

Start & End Page 
304 - 316
Received Date 
2013-06-19
Revised Date 
Accepted Date 
2013-08-21
Full Text 
  Download
Keyword 
Trichoderma harzianum, Biological control, Pathogenic fungi, Purification, Proteomics
Volume 
Vol.42 No.2 (APRIL 2015)
DOI 
Citation 
Phupiewkham W., Sirithorn P., Saksirirat W. and Thammasirirak S., Antibacterial Agents from Trichoderma harzianum strain T9 Against Pathogenic Bacteria , Chiang Mai J. Sci., 2015; 42(2): 304-316.
SDGs
View:562 Download:200

  RELATED ARTICLE

CFPG: Creating a Common Fungal Pathogenic Genes Database through Data Mining
Article ID: e2024038
Author:Kenneth Lee Shean Tan and Saharuddin Bin Mohamad
Vol.51 No.3 (May 2024) View: 745 Download:132
Biocontrol of Colletotrichum falcatum Causing Red Rot Disease of Sugarcane Using Non-toxigenic Aspergillus sp.
Article ID: e2023032
Author:Atcharaporn Srijudanu, Onuma Piasai, Wiphawee Leesutthiphonchai, Mattana Wanitch, Chingchai Chaisiri, Netnapis Khewkhom
Vol.50 No.3 (May 2023) View: 396 Download:335
Evaluation of Antifungal Activity from Bacillus subtilis strain AS80 Against Pathogenic Fungi Isolated from Hass Avocado Fruits
Article ID: e2023019
Author:Sugunya Suebsan, Chanikarn Mee-u-sah, Saowarat Suyana, Manlika Onprachoo, Kanta Sangwijit and Somboon Anantalabhochai
Vol.50 No.2 (March 2023) View: 433 Download:391
Biological Control of Brassicales Ring Spot Disease Caused by Alternaria brassicicola Using Antagonistic Yeasts
Article ID: e2023016
Author:Sukanya Haituk, Parichad Pakdeeniti, Patchareeya Withee, Anuruddha Karunarathna, Irish Claire Literatus, Jaturong Kumla, Ratchadawan Cheewangkoon, Jintana Suwannamanee and Jeerapa Nguanhom
Vol.50 No.2 (March 2023) View: 646 Download:414
Inhibition of Lasiodiplodia pseudotheobromae Causing Fruit Rot Disease of Longan by Using Antagonistic Bacillus siamensis RFCD306
Article ID: e2023004
Author:Aimalin Pipattanapuckdee, Pimjai Seehanam, Chantalak Tiyayon, Danai Boonyakait, Kaewalin Kunasakdakul, Salit Supakitthanakorn and On-Uma Ruangwong
Vol.50 No.1 (January 2023) View: 508 Download:420
SDGs:
Biocontrol Potential of Arbuscular Mycorrhizal Fungi on Soil-borne Pathogens in Rice and Other Crops
page: 730 - 741
Author:Ruwanthika Kalamulla, Samantha C. Karunarathna, Suhail Asad, Saowaluck Tibpromma, Steven L. Stephenson, Itthayakorn Promputtha and Neelamanie Yapa
Vol.49 No.3 (Special Issue II : May 2022) View: 914 Download:333
SDGs:
A New Species (Fuscosporella xingyiensis sp. nov.) and a New Record (Sporoschisma chiangraiense) of Wood- Decay Fungi from Guizhou, China
page: 718 - 729
Author:Xia Xu, Nalin N. Wijayawardene , Chunsheng Long, Xiaobing Li , Min Cheng, Di Wang, Meizhu Wang, Feng Wang, Yanxia Liu, Qirui Li and Yingqian Kang
Vol.49 No.3 (Special Issue II : May 2022) View: 826 Download:308
Endophytic Fungi from Oncosperma sp. with Promising In Vitro Plant Growth Promotion and Antagonistic Activities
page: 837 - 852
Author:Machima Saengket, Kevin D Hyde, Vinit Kumar, Mingkwan Doilom and Siraprapa Brooks
Vol.48 No.3 (Special Issue I : May 2021) View: 731 Download:432
SDGs:
Optimization, Purification and Characterization of b-xylanase by a Novel Thermotolerant Strain of Microbispora siamensis, DMKUA 245T
page: 2267 - 2282
Author:Antika Boondaeng, Sukhumaporn Krajangsang, Chanaporn Trakunjae, Thanasak Lomthong, Shinji Tokuyama and Vichien Kitpreechavanich
Vol.45 No.6 (September 2018) View: 519 Download:259
The Antagonistic Activity of Bioactive Compound Producing Streptomyces of Fusarium Wilt Disease and Sheath Blight Disease in Rice
page: 1680 - 1698
Author:Mathurot Chaiharn, Nikhom Sujada, Wasu Pathom-aree and Saisamorn Lumyong
Vol.45 No.4 (July 2018) View: 576 Download:192
Response Surface Methodology Based Optimized Purification of the Residual Glycerol from Biodiesel Production Process
page: 1570 - 1582
Author:Muhammad Danish*, Muhammad Waseem Mumtaz [a], Mahpara Fakhar [a] and Umer Rashid*
Vol.44 No.4 (October 2017) View: 627 Download:262
Production and Characterization of Bacteriocin-producing Bacteria Capable of Inhibition of Streptococcus dysgalactiae
page: 366 - 374
Author:Nalin Wongkattiya [a], Ian H. Fraser [b], Piyanuch Niamsup [a], Phanchana Sanguansermsri [c] and Don
Vol.44 No.2 (April 2017) View: 548 Download:175
Partial Purification and Comparison of Precipitation Techniques of Pyruvate Decarboxylase Enzyme
page: 184 - 192
Author:Julaluk Tangtua, Charin Techapun, Ronachai Pratanaphon, Ampin Kuntiya, Vorapat Sanguanchaipaiwong, T
Vol.44 No.1 (JANUARY 2017) View: 565 Download:210
Biochemical Characterization of Two Acid Phosphatases Purified from Breadfruit (Artocarpus communis) Seeds
page: 1100 - 1112
Author:Hubert Kouassi Konan*, Desire Yapi Assoi Yapi, Clement Yao Yue Bi, Thierry Fankroma Martial Kone , Parfait Eugene Jean N’guessan Kouadio and Lucien Patrice Kouame
Vol.43 No.5 (OCTOBER 2016) View: 495 Download:127
Purification and Evaluation of Bioactive Fractions for Anti-Cancer Potentials from the Flowers and Leaves of Nyctanthes arbor-tristis L.
page: 100 - 111
Author:Bibechana Timsina[a] and Varalakshmi Kilingar Nadumane*[a]
Vol.43 No.1 (JANUARY 2016) View: 986 Download:225
Assessing the Impact of Dominant Sequencing-Based Gene Expression Profiling Techniques (SGEPTs) on Phytopathogenic Fungi
page: 922 - 944
Author:Dawei Wang and Youliang Peng*
Vol.41 No.4 (SPECIAL ISSUE 1) View: 469 Download:137
Purification and Characterization of Fungal Laccase from Mycena purpureofusca
page: 151 - 160
Author:Shujing Sun1, Yonghui Zhang1, Youxiong Que2, Bixian Liu1, Kaihui Hu1, and Liping Xu2*
Vol.40 No.2 (APRIL 2013) View: 523 Download:145
Optimization of Solid State Fermentation for Reducing Sugar Production from Agricultural Residues of Sweet Sorghum by Trichoderma harzianum
page: 270 - 280
Author:Anusith Thanapimmetha, Korsuk Vuttibunchon, Boosaree Titapiwatanakun and Penjit Srinophakun
Vol.39 No.2 (APRIL 2012) View: 452 Download:150
The need for re-inventory of Thai phytopathogens
page: 625 - 637
Author:Thida win Ko Ko, Eric Hugh Charles McKenzie, Ali Hassan Bahkali, Chaiwat To-anun, Ekachai
Vol.38 No.4 (OCTOBER 2011) View: 480 Download:265
Cloning and Expression of Chitinase Gene Isolated from Insect Pathogenic Fungi, Beauveria bassiana in Escherichia coli
page: 347 - 355
Author:Khemika Songjang*[a], Tawee Donchai [a], Paranya Chaiyawat [a] and Christopher R. Meyer [b]
Vol.33 No.3 (SEPTEMBER 2006) View: 496 Download:135
Simple Purification of Indirubin from Indigofera tinctoria Linn. and Inhibitory Effect on MCF-7 Human Breast Cancer Cells
page: 329 - 337
Author:Paitoon Aobchey , Supachok Sinchaikul , Suree Phutrakul and Shui-Tein Chen
Vol.34 No.3 (SEPTEMBER 2007) View: 529 Download:178
A Rapid Process for Purification of Chitinase from the Latex of Carica papaya
page: 237 - 242
Author:Sarote Nitsawang [a] and Pawinee Kanasawud* [b]
Vol.33 No.2 (MAY 2006) View: 458 Download:146
Optimising the Purification of Terpyridine-Cytochrome c Bioconjugates
page: 236 - 246
Author:Joshua R. Peterson, and Pall Thordarson
Vol.36 No.2 (MAY 2009) View: 506 Download:168



Search in this journal


Document Search


Author Search

A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z

Popular Search






Chiang Mai Journal of Science

Faculty of Science, Chiang Mai University
239 Huaykaew Road, Tumbol Suthep, Amphur Muang, Chiang Mai 50200 THAILAND
Tel: +6653-943-467




Faculty of Science,
Chiang Mai University




EMAIL
cmjs@cmu.ac.th




Copyrights © Since 2021 All Rights Reserved by Chiang Mai Journal of Science