Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

1,647
Articles
Q3 0.80
Impact Factor
Q3 1.3
CiteScore
7 days
Avg. First Decision
Outline
Figures and Tables
ARTICLE METRICS
Views 966
Downloads 1,124

Evaluation of Antifungal Activity from Bacillus subtilis strain AS80 Against Pathogenic Fungi Isolated from Hass Avocado Fruits

Sugunya Suebsan, Chanikarn Mee-u-sah, Saowarat Suyana, Manlika Onprachoo, Kanta Sangwijit and Somboon Anantalabhochai
* Author for corresponding; e-mail address: sugunya.pi@up.ac.th
Volume: Vol.50 No.2 (March 2023)
Research Article
DOI: https://doi.org/10.12982/CMJS.2023.019
Received: 14 December 2022, Revised: 19 Febuary 2023, Accepted: 21 Febuary 2023, Published: -

Citation: Suebsan S., Mee-u-sah C., Suyana S., Onprachoo M., Sangwijit K. and Anantalabhochai S., Evaluation of Antifungal Activity from Bacillus subtilis strain AS80 Against Pathogenic Fungi Isolated from Hass Avocado Fruits, Chiang Mai Journal of Science, 2023; 50(2): e2023019. DOI 10.12982/CMJS.2023.019.

Abstract

      Fruit rot and anthracnose are the main diseases in avocado fruits caused by fungal species. Solving this problem with chemical reagents has led to human health and pollution problems. Therefore, this study was aimed to select antagonistic bacteria to control those pathogenic fungi. The pathogenic fungi were isolated from avocado fruit occurred fruit rot or anthracnose symptoms. Based on morphological characteristics and the BLASTn results of sequences of the internal transcribed spacer of ribosomal DNA, the obtained fungal isolates belonged to three fungal genera, namely Fusarium, Lasiodiplodia and Colletotrichum, which was the most commonly occurring. Pathogenicity tests were evaluated on avocado fruit. Then the rhizosphere bacteria were isolated from the avocado rhizosphere. To study antagonistic activity, 101 bacterial isolates were cultured with five fungal pathogens using dual culture technique. The bacterial isolate AS80 exhibited high potential to against Fusarium sp. (61.7%), Lasiodiplodia sp. (66.7%) and Colletotrichum sp. (74.1%). The strain AS80 was used to evaluate the efficacy control of Fusarium sp. on “Hass” avocado fruit. The in vivo evaluation revealed that AS80 showed high efficacy against Fusarium sp. The 16s ribosomal DNA sequencing indicated that AS80 was similar to Bacillus subtilis (99.53 %). Further study under field conditions should be done to validate their efficacy.

Keywords: Anthracnose, biological control, fruit rot, Persea americana, plant pathogen

Related Articles

Efficacy of Solid Formulation of Trichoderma virens in Managing Cowpea Crown Rot Caused by Agroathelia rolfsii (Sacc.)
DOI: 10.12982/CMJS.2025.077.

Gannile Herath Mudiyanselage Gedara Sajini Wasundara Nawarathna, Tikka Dewage Chamarika Priyadarshani, Weerawickramage Chaminda Priyanka Egodawatta, Garumuni Dilrukshi Nadeeshani Manike, Entaj Tarafder, Abdallah M. Elgorban, Wenhua Lu and Samantha C. Karunarathna

Vol.52 No.5 (September 2025)
Research Article View: 430 Download: 309
Development of Loop-Mediated Isothermal Amplification (LAMP) Assay for Anthracnose Disease Caused by Colletotrichum asianum and Colletotrichum musae
DOI: 10.12982/CMJS.2025.074.

Kanittha Chansri, Anupan Kongbangkerd and Maliwan Nakkuntod

Vol.52 No.5 (September 2025)
Research Article View: 613 Download: 150
A Rhizobacterium Kitasatospora nipponensis Demonstrates Antifungal Activity against Basal Stem Rot Disease and Promotes Plant Growth in Oil Palm (Elaeis guineensis) Seedlings
DOI: 10.12982/CMJS.2025.033.

Anurag Sunpapao, Supaporn Pithakkit and Prisana Wonglom

Vol.52 No.3 (May 2025)
Research Article View: 752 Download: 332
A New Approach for Machine Learning-Based Recognition of Meat Species Using a BME688 Gas Sensors Matrix
DOI: 10.12982/CMJS.2025.031.

Nursel Söylemez Milli, İsmail Hakkı Parlak and Mehmet Milli

Vol.52 No.3 (May 2025)
Research Article View: 769 Download: 820
Biocontrol of Colletotrichum falcatum Causing Red Rot Disease of Sugarcane Using Non-toxigenic Aspergillus sp.
DOI: 10.12982/CMJS.2023.032.

Atcharaporn Srijudanu, Onuma Piasai, Wiphawee Leesutthiphonchai, Mattana Wanitch, Chingchai Chaisiri, Netnapis Khewkhom

Vol.50 No.3 (May 2023)
Research Article View: 2,353 Download: 675
Biological Control of Brassicales Ring Spot Disease Caused by Alternaria brassicicola Using Antagonistic Yeasts
DOI: 10.12982/CMJS.2023.016.

Sukanya Haituk, Parichad Pakdeeniti, Patchareeya Withee, Anuruddha Karunarathna, Irish Claire Literatus, Jaturong Kumla, Ratchadawan Cheewangkoon, Jintana Suwannamanee and Jeerapa Nguanhom

Vol.50 No.2 (March 2023)
Research Article View: 1,786 Download: 877
Inhibition of Lasiodiplodia pseudotheobromae Causing Fruit Rot Disease of Longan by Using Antagonistic Bacillus siamensis RFCD306
DOI: 10.12982/CMJS.2023.004.

Aimalin Pipattanapuckdee, Pimjai Seehanam, Chantalak Tiyayon, Danai Boonyakait, Kaewalin Kunasakdakul, Salit Supakitthanakorn and On-Uma Ruangwong

Vol.50 No.1 (January 2023)
Research Article View: 1,584 Download: 839
Biocontrol Potential of Arbuscular Mycorrhizal Fungi on Soil-borne Pathogens in Rice and Other Crops
DOI: 10.12982/CMJS.2022.057.

Ruwanthika Kalamulla, Samantha C. Karunarathna, Suhail Asad, Saowaluck Tibpromma, Steven L. Stephenson, Itthayakorn Promputtha and Neelamanie Yapa

Vol.49 No.3 (Special Issue II : May 2022)
Research Article View: 2,021 Download: 646
A New Species (Fuscosporella xingyiensis sp. nov.) and a New Record (Sporoschisma chiangraiense) of Wood- Decay Fungi from Guizhou, China
DOI: 10.12982/CMJS.2022.056.

Xia Xu, Nalin N. Wijayawardene , Chunsheng Long, Xiaobing Li , Min Cheng, Di Wang, Meizhu Wang, Feng Wang, Yanxia Liu, Qirui Li and Yingqian Kang

Vol.49 No.3 (Special Issue II : May 2022)
Research Article View: 2,004 Download: 531
Endophytic Fungi from Oncosperma sp. with Promising In Vitro Plant Growth Promotion and Antagonistic Activities
page: 837 - 852

Machima Saengket, Kevin D Hyde, Vinit Kumar, Mingkwan Doilom and Siraprapa Brooks

Vol.48 No.3 (Special Issue I : May 2021)
Research Article View: 1,718 Download: 1,436
Phytopathogenic Fungi Identification in Thailand: Current Trends and Future Perspectives
page: 726 - 759

Ratchadawan Cheewangkoon, Rampai Kodsueb, Sararat Monkhung, Kapuduwa Vitharanage Anuruddha Karunarathna, Thitima Wongwan, Patchareeya Withee, Sukanya Haituk and Nisachon Tamakeaw

Vol.48 No.3 (Special Issue I : May 2021)
Review Article View: 1,895 Download: 876
The Antagonistic Activity of Bioactive Compound Producing Streptomyces of Fusarium Wilt Disease and Sheath Blight Disease in Rice
page: 1680 - 1698

Mathurot Chaiharn, Nikhom Sujada, Wasu Pathom-aree and Saisamorn Lumyong

Vol.45 No.4 (July 2018)
Research Article View: 1,028 Download: 446
Antibacterial Agents from Trichoderma harzianum strain T9 Against Pathogenic Bacteria
page: 304 - 316

Weeraya Phupiewkham, Pisan Sirithorn, Weerasak Saksirirat, Sompong Thammasirirak

Vol.42 No.2 (APRIL 2015)
Research Article View: 1,473 Download: 302
The need for re-inventory of Thai phytopathogens
page: 625 - 637

Thida win Ko Ko, Eric Hugh Charles McKenzie, Ali Hassan Bahkali, Chaiwat To-anun, Ekachai

Vol.38 No.4 (OCTOBER 2011)
Research Article View: 1,340 Download: 610
Outline
Figures