Chiang Mai Journal of Science

Paper Type 

Title 

Author 

Email 

A chitinase gene (1047 bp) was amplified from Beauveria bassiana BCC3653 genomic DNA by PCR technique using designed primers to generate 5’-EcoRI and 3’-HindIII ends. As this gene contains no intron, the PCR product was cloned into an expression vector, pSE420 resulting a recombinant plasmid, pDSM1. This recombinant plasmid was then transformed into Escherichia coli TOP10 cells. After confirming the DNA sequence of cloned PCR, gene expression was induced by IPTG. The chitinase activities were then investigated in gel supplemented with glycol chitin. Two chitinolytic activity bands were detected only in E. coli carrying pDSM1 suggesting the expression of cloned chitinase gene.

Start & End Page 

Received Date 

Revised Date 

Accepted Date 

Full Text 

Keyword 

Volume 

DOI 

Citation 

SDGs