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Cloning and Expression of Chitinase Gene Isolated from Insect Pathogenic Fungi, Beauveria bassiana in Escherichia coli


Paper Type 
Contributed Paper
Title 
Cloning and Expression of Chitinase Gene Isolated from Insect Pathogenic Fungi, Beauveria bassiana in Escherichia coli
Author 
Khemika Songjang*[a], Tawee Donchai [a], Paranya Chaiyawat [a] and Christopher R. Meyer [b]
Email 
khemika@chiangmai.ac.th
Abstract:
A chitinase gene (1047 bp) was amplified from Beauveria bassiana BCC3653 genomic DNA by PCR technique using designed primers to generate 5’-EcoRI and 3’-HindIII ends. As this gene contains no intron, the PCR product was cloned into an expression vector, pSE420 resulting a recombinant plasmid, pDSM1. This recombinant plasmid was then transformed into Escherichia coli TOP10 cells. After confirming the DNA sequence of cloned PCR, gene expression was induced by IPTG. The chitinase activities were then investigated in gel supplemented with glycol chitin. Two chitinolytic activity bands were detected only in E. coli carrying pDSM1 suggesting the expression of cloned chitinase gene.
Start & End Page 
347 - 355
Received Date 
2006-02-15
Revised Date 
Accepted Date 
2006-07-21
Full Text 
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Keyword 
Beauveria bassiana, chitinase, gene cloning, insect pathogenic fungi
Volume 
Vol.33 No.3 (SEPTEMBER 2006)
DOI 
Citation 
Songjang K., Donchai T., Chaiyawat P. and Meyer C., Cloning and Expression of Chitinase Gene Isolated from Insect Pathogenic Fungi, Beauveria bassiana in Escherichia coli, Chiang Mai J. Sci., 2006; 33(3): 347-355.
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Chiang Mai Journal of Science

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