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Cloning and Expression of Phytoene Desaturase Gene from Undaria pinnatifida Suringar


Paper Type 
 Contributed Paper
Title 
 Cloning and Expression of Phytoene Desaturase Gene from Undaria pinnatifida Suringar
Author 
 Tengteng Guan, Tingting Zhang, Yi Ma, Yinjie Li, Guicai Du* and Ronggui Li*
Email 
 duguicai@qdu.edu.cn; lrg@qdu.edu.cn
Abstract:
The phytoene desaturase is a key enzyme involved in the carotenoids biosynthesis pathway,
which catalyzes the conversion of ζ-carotene from phytoene. Undaria pinnatifida Suringar is an edible
economic brown algae that produces high concentrations of fucoxanthin. In this study, the PDS gene
(UpPDS) was isolated and characterized from U. pinnatifida, and the full-length cDNA sequence was
1707 bp in length and encoded 568 amino acid residues. Furthermore, the putative protein sequence
showed a high homology to the PDS from other known plants. Phylogenetic analysis showed that
the UpPDS was more related to that of brown algae compared to other species. By heterologous
complementation and high-performance liquid chromatography analysis, desaturation activity of
recombinant UpPDS was confirmed by production of ζ-carotene in engineering E. coli co-transformed
with crtE, crtB and UpPDS. The L9(3 4) orthogonal test was used to optimize ζ-carotene fermentation
conditions of the engineering bacteria. The results showed that temperature had the most significant
effect on ζ-carotene production, the maximal yield of ζ-carotene could reach 5.0mg/L under the
optimal conditions.
Start & End Page 
 1118 - 1129
Received Date 
 2019-07-02
Revised Date 
Accepted Date 
 2020-06-18
Full Text 
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Keyword 
 phytoene desaturase, gene cloning, ζ-carotene, expression, Undaria pinnatifida Suringar
Volume 
 Vol.47 No.6 (November 2020)
DOI 
Citation 
 Guan T., Zhang T., Ma Y., Li Y., Du G. and Li R., Cloning and Expression of Phytoene Desaturase Gene from Undaria pinnatifida Suringar, Chiang Mai J. Sci., 2020; 47(6): 1118-1129.
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