Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

1,647
Articles
Q3 0.80
Impact Factor
Q3 1.3
CiteScore
7 days
Avg. First Decision
Outline
Figures and Tables
ARTICLE METRICS
Views 1,362
Downloads 645

Investigation on Selection, Improvement of Biomass Production and Protein Content in Duckweeds (Lemna Strains)

Ramesh Basnet, Anping Du, Li Tan, Ling Guo, Yanglin Jin, Zhuolin Yi, Tianfang Huang, Yang Fang and Hai Zhao
* Author for corresponding; e-mail address: fangyang@cib.ac.cn, zhaohai@cib.ac.cn
Volume: Vol.51 No.4 (July 2024)
Research Article
DOI: https://doi.org/10.12982/CMJS.2024.065
Received: 12 December 2023, Revised: 19 May 2024, Accepted: 21 June 2024, Published: -

Citation: Basnet R., Du A., Tan L., Guo L., Jin Y., Yi Z., et al., Investigation on selection, improvement of biomass production and protein content in duckweeds (Lemna Strains), Chiang Mai Journal of Science, 2024; 51(4): e2024065. DOI 10.12982/CMJS.2024.065.

Abstract

     Duckweeds are becoming an emerging plant protein source as it may be produced in a more efficient and eco-friendly manner than other major crops. Screening is essential for tapping the desired traits. We conducted the screening on 32 Lemna species and accessions available in the Gene Bank. It follows two steps screening, primary screening involved culturing in 1/5 strength Hoagland Solution (HS) for one week. Based on protein %, the best three strains were cultured two sets ammonium and nitrate nitrogen source. Based on both biomass and protein content, D0158 strain was identified as the best. Then, the strain was optimized in five different parameters. In our study, we found that a longer photoperiod resulted in greater biomass accumulation and a lower percentage of protein. There was a 6.51% increase in crude protein, rising from 30.97% to 33.13% and dry biomass growth rate increase of 54.48% from 4.35 to 6.72 g/m2/d. The findings can be used to enhance the duckweed indoor cultivation system. Additionally, this protocol can be applied to other duckweed strains for similar purposes.

Keywords: screening, Lemna, biomass, protein, optimization

Related Articles

Isolation and Identification of Cd-resistant Bacteria Ld-1 in Karst Mining Regions and Its Microbial Biosorption Mechanisms for Cd
DOI: 10.12982/CMJS.2026.020.

Dan Li, Fumin Zuo, Jiayu Tian, Yichi Zhang, Qinghai Zhang, Yuan Tang* and Jianzhong Cheng

Vol.53 No.1 (January 2026)
Research Article View: 301 Download: 23
Enhancing Butanol during Acetone-butanol-ethanol (ABE) Fermentation using Clostridium beijerinckii ATCC 10132 by pH Control Strategy and Molasses Pretreatment
DOI: 10.12982/CMJS.2025.069.

Sireethorn Khaonuan, Nikannapas Usmanbaha, Chen-Yeon Chu, Rattana Jariyaboon, Nils-Kåre Birkeland and Prawit Kongjan

Vol.52 No.5 (September 2025)
Research Article View: 795 Download: 214
Characterization, Compositional Analysis and Calorific Value of Terminalia ivorensis Sawdust, Corncob and Low-Density Polyethylene as Potential Blended Feedstock for Bio-oil Production
DOI: 10.12982/CMJS.2025.056.

Sakina Bello, Taofik Olatunde Uthman, Serdar Surgun and Abdullahi Muhammad Sokoto

Vol.52 No.4 (July 2025)
Research Article View: 789 Download: 154
Microscopy Investigation of Streblus asper Lour. Leaves-derived SiO2/C with Polypyrrole Nanocomposites as Sustainable Anode Materials for Lithium-ion Batteries
DOI: 10.12982/CMJS.2024.099.

Kittiched Khunpakdee, Thanapat Autthawong, Natthakan Ratsameetammajak and Thapanee Sarakonsri

Vol.51 No.6 (November 2024)
Research Article View: 1,090 Download: 381
H2S Removal from Biogas using Steel Wool (Fe2O3) Adsorption Combined with Water Absorption: Experimental and Modelling via RSM
DOI: 10.12982/CMJS.2024.027.

Jiraporn Paoaiang, Prukraya Pongyeela, Nirana Chairerk and Juntima Chungsiriporn

Vol.51 No.2 (March 2024)
Research Article View: 1,786 Download: 1,739
Grey-Taguchi Approach for Optimizing FSW Parameters in Joining AA6262 and AA5083 Alloys
DOI: 10.12982/CMJS.2024.003.

Jeyakrishnan Sundararaj, Movva Naga Swapna Sri, Anusha Peyyala and Vijayakumar Sivasundar

Vol.51 No.1 (January 2024)
Research Article View: 1,835 Download: 2,164
Local Search Element Decomposition Method to Solve Integer and m-Integer Problems
DOI: 10.12982/CMJS.2022.077.

Ekkaphon Jaiyen, Komgrit Leksakul and Nivit Charoenchai

Vol.49 No.4 (July 2022)
Research Article View: 1,555 Download: 912
Capacitance Optimization of Planar Carbon-based Supercapacitor
page: 607 - 613

Ukrit Jitropas, Chumphol Yunphuttha, Sumeth Siriroj and Worawat Meevasana*

Vol.47 No.4 (Special Issue II : July 2020)
Research Article View: 2,325 Download: 1,242
Optimization of Culture Conditions for Oil Production by the Double Mutant of Chlorella sorokiniana DMKU5202-D223
page: 1055 - 1068

Mathurin Vichachucherd, Wichien Yongmanitchai and Duenrut Chonudomkul *

Vol.46 No.6 (November 2019)
Research Article View: 1,670 Download: 493
Bioproduction of Xylitol From Chestnut Shell Hydrolysate: Optimization of Fermentation Parameters
page: 822 - 838

Kubra Eryasar-Orer and Seda Karasu-Yalcin*

Vol.46 No.5 (September 2019)
Research Article View: 1,151 Download: 350
Optimization of Oleic Acid Esterification for Biodiesel Production using Brønsted Acidic Ionic Liquid as a Catalyst
page: 714 - 726

Benyong Han, Fang Yin*, Shiqing Liu, Xingling Zhao, Jing Liu, Changmei Wang, Hong Yang, Wudi Zhang*

Vol.46 No.4 (July 2019)
Research Article View: 1,163 Download: 647
Isolation of A Novel Collagenase-producing Strain from Animal Bone Wastes and Optimization of Its Enzyme Production
page: 219 - 235

Lili Liu*, Yuanyuan Meng, Xiaoning Dai and Ke Chen

Vol.46 No.2 (March 2019)
Research Article View: 1,110 Download: 333
Optimization and Performance of Covered-Light Structures for Hydrogen Production by Rhodopseudomonas palustris TN1
page: 2597 - 2608

Pornnipa Botthong, Tanatorn Pornsawatchai, Wanticha Savedboworn, Sriwiang Tipkanon, Atipan Siammai and Wiboon Riansa-ngawong

Vol.45 NO.7 (November 2018)
Research Article View: 1,302 Download: 295
Toluene Oxidation using K-OMS 2 Synthesized Via Hydrothermal Process by Central Composite Design
page: 1030 - 1038

Jessa Marie Millanar, Mark Daniel de Luna, Aummara Yodsa-nga and Kitirote Wantala*

Vol.45 No.2 (March 2018)
Research Article View: 1,130 Download: 228
Screening and Optimization of Squalene Production from Microalgae Aurantiochytrium sp.
page: 680 - 691

Aeujkom Saengwong, Wichien Yongmanitchai and Duenrut Chonudomkul*

Vol.45 No.2 (March 2018)
Research Article View: 1,180 Download: 362
Hydrocarbon Production and Biodiesel Properties of a Green Microalga Botryococcus braunii KMITL 2 Cultivated Outdoor in Open Pond and Closed Photobioreactor
page: 668 - 679

Suneerat Ruangsomboon

Vol.45 No.2 (March 2018)
Research Article View: 1,002 Download: 389
Biohydrogen Production from Crude Glycerol Using Anaerobic Mixed Cultures: Media Compositions Optimization
page: 653 - 667

Sureewan Sittijunda and Alissara Reungsang*

Vol.45 No.2 (March 2018)
Research Article View: 1,158 Download: 363
Ultrasonic Extraction, Antioxidant and Anti-glycation Activities of Polysaccharides from Gynura divaricata Leaves
page: 144 - 156

Pawinee Deetae*[a, e], Kwanrudee Tseng [a], Chanathinart Pasuphan [a], Atikorn Panya [b], Pawadee Methacanon [c], Waranya Temthawee [a] and Manop Suphantharika [d]

Vol.44 No.1 (JANUARY 2017)
Research Article View: 2,444 Download: 416
Optimization of Xylitol Production by Candida tropicalis A26
page: 50 - 58

Wanlapa Lorliam [a], Ancharida Akaracharanya [b], Sukhumaporn Krajangsang [c], Vasana Tolieng [d] a

Vol.44 No.1 (JANUARY 2017)
Research Article View: 1,208 Download: 257
Utilization of Non-rubber Skim Latex for Poly(L-lactide)-degrading Enzyme Production by Actinomadura keratinilytica Strain T16-1
page: 1016 - 1026

Sabadee Worrathampitak [a], Shinji Tokuyama [b], Vichien Kitpreechavanich [c] and Sukhumaporn Sukkhum* [a]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,161 Download: 369
Molecular Docking Study of Mycobacterium tuberculosis Dihydrofolate Reductase in Complex with 2,4-diaminopyrimidines Analogues
page: 931 - 945

Pimonluck Sittikornpaiboon [a], Pisanu Toochinda [a], Chawanee Thongpanchang [b], Ubolsree Leartsakulpanich [b] and Luckhana Lawtrakul* [a]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,024 Download: 373
Detention Time Study of Algal Biomass Production with Natural Water Medium
page: 549 - 559

Rameshprabu Ramaraj, David Da-Wei Tsai and Paris Honglay Chen

Vol.42 No.3 (JULY 2015)
Research Article View: 1,031 Download: 287
Improvement of production and stability of silk degumming protease by Bacillus sp. C4 SS-2013
page: 599 - 613

Nisa Romsomsa, Patoomporn Chim-anage, Sarote Sirisansaneeyakul

Vol.42 No.3 (JULY 2015)
Research Article View: 1,466 Download: 291
Optimization of Solid-state Fermentation for Fruiting Body Growth and Cordecepin Production by Cordyceps militaris
page: 858 - 872

Ting-chi Wen, Cuang-rong Li, Ji-chuan Kang*,Chao Kang and Kevin D. Hyde

Vol.41 No.4 (SPECIAL ISSUE 1)
Research Article View: 1,475 Download: 590
Chlorophyll is not accurate measurement for algal biomass
page: 547 - 555

Rameshprabu Ramaraj **[a], David D-W. Tsai [a] and Paris Honglay Chen *[a]

Vol.40 No.4 (OCTOBER 2013)
Research Article View: 1,000 Download: 303
Screening of 50 Microbial Strains for Production of Ethanol and (R)-phenylacetylcarbinol
page: 299 - 304

Julaluk Tangtua [a], Charin Techapun [b], Ronachai Pratanaphon [b], Ampin Kuntiya [b], Thanongsak C

Vol.40 No.2 (APRIL 2013)
Research Article View: 978 Download: 272
Optimization of Media Components and Operating Conditions for Exogenous Production of Fungal L-asparaginase
page: 270 - 279

Gurunathan Baskar [a], and Sahadevan Renganathan*[b]

Vol.38 No.2 (APRIL 2011)
Research Article View: 1,043 Download: 238
Phenolic Compounds in Protein Fraction of Processed Mustard Meal
page: 1 - 8

Worravimon Trakarnsirinont and Nuansri Rakariyatham

Vol.27 No.1 (JUNE 2000)
Opinion View: 1,064 Download: 302
Outline
Figures