Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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Investigation on Selection, Improvement of Biomass Production and Protein Content in Duckweeds (Lemna Strains)

Ramesh Basnet, Anping Du, Li Tan, Ling Guo, Yanglin Jin, Zhuolin Yi, Tianfang Huang, Yang Fang and Hai Zhao
* Author for corresponding; e-mail address: fangyang@cib.ac.cn, zhaohai@cib.ac.cn
Volume: Vol.51 No.4 (July 2024)
Research Article
DOI: https://doi.org/10.12982/CMJS.2024.065
Received: 12 December 2023, Revised: 19 May 2024, Accepted: 21 June 2024, Published: -

Citation: Basnet R., Du A., Tan L., Guo L., Jin Y., Yi Z., et al., Investigation on Selection, Improvement of Biomass Production and Protein Content in Duckweeds (Lemna Strains), Chiang Mai Journal of Science, 2024; 51(4): e2024065. DOI 10.12982/CMJS.2024.065.

Abstract

     Duckweeds are becoming an emerging plant protein source as it may be produced in a more efficient and eco-friendly manner than other major crops. Screening is essential for tapping the desired traits. We conducted the screening on 32 Lemna species and accessions available in the Gene Bank. It follows two steps screening, primary screening involved culturing in 1/5 strength Hoagland Solution (HS) for one week. Based on protein %, the best three strains were cultured two sets ammonium and nitrate nitrogen source. Based on both biomass and protein content, D0158 strain was identified as the best. Then, the strain was optimized in five different parameters. In our study, we found that a longer photoperiod resulted in greater biomass accumulation and a lower percentage of protein. There was a 6.51% increase in crude protein, rising from 30.97% to 33.13% and dry biomass growth rate increase of 54.48% from 4.35 to 6.72 g/m2/d. The findings can be used to enhance the duckweed indoor cultivation system. Additionally, this protocol can be applied to other duckweed strains for similar purposes.

Keywords: screening, Lemna, biomass, protein, optimization

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Research Article View: 855 Download: 265
Application of Frequency Domain Experiments in the Factor Screening of Production Systems
page: 171 - 190

Watha Minsan

Vol.39 No.2 (APRIL 2012)
Research Article View: 867 Download: 439
Fluid Flow Induced Shear Stress Affects Cell Growth and Total Flavone Production by Phellinus igniarius in Stirred-Tank Bioreactor
page: 69 - 75

Hu Zhu, Wei Liu, Baozhen Tian and Shuaisshuai Zhang

Vol.39 No.1 (JANUARY 2012)
Research Article View: 956 Download: 272
Optimization of Media Components and Operating Conditions for Exogenous Production of Fungal L-asparaginase
page: 270 - 279

Gurunathan Baskar [a], and Sahadevan Renganathan*[b]

Vol.38 No.2 (APRIL 2011)
Research Article View: 968 Download: 231
Optimization of Micro Metal Injection Molding with Multiple Performance Characteristics using Grey Relational Grade
page: 231 - 241

Mohd H.I. Ibrahim*[a,b], Norhamidi Muhamad [b], Abu B. Sulong [b], Khairur R. Jamaludin [c], Nor H.

Vol.38 No.2 (APRIL 2011)
Research Article View: 988 Download: 250
A Comparative Analysis of Conjugate Gradient Algorithms & PSO Based Neural Network Approaches for Reusability Evaluation of Procedure Based Software Systems
page: 123 - 135

Parvinder S. Sandhu*[a], Shalini Chhabra [b]

Vol.38 (SPECIAL ISSUE 2011)
Research Article View: 1,008 Download: 217
An Improved Algorithm for Protein Structural Comparison Based on Graph Theoretical Approach
page: 71 - 81

Cheng-Hsien Hsu [a], Sheng-Lung Peng*[b], and Yu-Wei Tsay [b]

Vol.38 (SPECIAL ISSUE 2011)
Research Article View: 1,998 Download: 279
Sunflower Seed Meal as Rumen-undegradable Protein Sources For Lactating Dairy Cows Fed Urea-treated Rice Straw
page: 119 - 125

Pramote Paengkoum

Vol.34 No.1 (JANUARY 2007)
Research Article View: 955 Download: 264
Development of Fuel Briquettes from Biomass-Lignite Blends
page: 43 - 50

Suparin Chaiklangmuang, Suwit Supa and Prattana Kaewpet

Vol.35 No.1 (JANUARY 2008)
Research Article View: 914 Download: 324
Preliminary Screening of Glycoprotein Profiles in Human Normal and Lung Cancer Sera by Different Staining Methods
page: 331 - 344

Piyorot Hongsachart, Supachok Sinchaikul, Suree Phutrakul and Shui-Tein Chen

Vol.35 No.2 (MAY 2008)
Research Article View: 910 Download: 248
Proteomic Analysis of Deglycosylated Proteins in Normal Human Serum Using Anhydrous Hydrogen Fluoride Treatment
page: 311 - 323

Supawadee Sriyam, Supachok Sinchaikul, Payungsak Tantipaiboonwong, Suree Phutrakul and Shui-Tein Che

Vol.35 No.2 (MAY 2008)
Research Article View: 967 Download: 254
Comparative Membrane Extraction Methods for Identifying Membrane Proteome of SW900 Squamous Lung Cancer Cell Line
page: 467 - 482

Piyorot Hongsachart, Supachok Sinchaikul, Suree Phutrakul,Weerah Wongkham and Shui-Tein Chen

Vol.35 No.3 (SEPTEMBER 2008)
Research Article View: 917 Download: 282
Optimising the Purification of Terpyridine-Cytochrome c Bioconjugates
page: 236 - 246

Joshua R. Peterson, and Pall Thordarson

Vol.36 No.2 (MAY 2009)
Short Communication View: 979 Download: 286
Chemical Analysis of Airborne Particulates for Air Pollutants in Chiang Mai and Lamphun Provinces, Thailand
page: 123 - 135

Somporn Chantara, Sunanta Wangkarn, Urai Tengcharoenkul, Walaya Sangchan and Mongkon Rayanakorn

Vol.36 No.2 (MAY 2009)
Short Communication View: 1,039 Download: 270
Immunoproteomic Assay of Streptococcus suis Serotype 2 Isolated from Patients
page: 134 - 150

Kanruethai Wongsawan, Aphinya Wongpia, Khemika Lomthaisong, Anuson Boonthum, Volaluck Supjatura, and

Vol.37 No.1 (JANUARY 2010)
Research Article View: 906 Download: 290
Sensitivity Enhancement of Differential Pulse Polarographic Technique by Simplex Optimization Method
page: 71 - 81

Buabarn Poonperm, Waret Veerasai* and Prapin Wilairat

Vol.27 No.2 (DECEMBER 2000)
Opinion View: 958 Download: 250
Phenolic Compounds in Protein Fraction of Processed Mustard Meal
page: 1 - 8

Worravimon Trakarnsirinont and Nuansri Rakariyatham

Vol.27 No.1 (JUNE 2000)
Opinion View: 983 Download: 302
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