Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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Investigation on Selection, Improvement of Biomass Production and Protein Content in Duckweeds (Lemna Strains)

Ramesh Basnet, Anping Du, Li Tan, Ling Guo, Yanglin Jin, Zhuolin Yi, Tianfang Huang, Yang Fang and Hai Zhao
* Author for corresponding; e-mail address: fangyang@cib.ac.cn, zhaohai@cib.ac.cn
Volume: Vol.51 No.4 (July 2024)
Research Article
DOI: https://doi.org/10.12982/CMJS.2024.065
Received: 12 December 2023, Revised: 19 May 2024, Accepted: 21 June 2024, Published: -

Citation: Basnet R., Du A., Tan L., Guo L., Jin Y., Yi Z., et al., Investigation on selection, improvement of biomass production and protein content in duckweeds (Lemna Strains), Chiang Mai Journal of Science, 2024; 51(4): e2024065. DOI 10.12982/CMJS.2024.065.

Abstract

     Duckweeds are becoming an emerging plant protein source as it may be produced in a more efficient and eco-friendly manner than other major crops. Screening is essential for tapping the desired traits. We conducted the screening on 32 Lemna species and accessions available in the Gene Bank. It follows two steps screening, primary screening involved culturing in 1/5 strength Hoagland Solution (HS) for one week. Based on protein %, the best three strains were cultured two sets ammonium and nitrate nitrogen source. Based on both biomass and protein content, D0158 strain was identified as the best. Then, the strain was optimized in five different parameters. In our study, we found that a longer photoperiod resulted in greater biomass accumulation and a lower percentage of protein. There was a 6.51% increase in crude protein, rising from 30.97% to 33.13% and dry biomass growth rate increase of 54.48% from 4.35 to 6.72 g/m2/d. The findings can be used to enhance the duckweed indoor cultivation system. Additionally, this protocol can be applied to other duckweed strains for similar purposes.

Keywords: screening, Lemna, biomass, protein, optimization

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