No Session
Chiang Mai Journal of Science, Faculty of Science, Chiang Mai University
 


Journal Volumes


  JOURNAL DETAIL



Regulation of Reactive Oxygen Species Metabolism by Trehalose Application Related to Alleviate Chilling Injury Development in Harvested ‘Kim Ju’ Guava Fruit


Paper Type 
Contributed Paper
Title 
Regulation of Reactive Oxygen Species Metabolism by Trehalose Application Related to Alleviate Chilling Injury Development in Harvested ‘Kim Ju’ Guava Fruit
Author 
Thanakorn Vichaiya, Sirawich Chotikakham and Sitthisak Intarasit
Email 
sitthisak.inta@cmu.ac.th
Abstract:

Chilling injury (CI) in postharvest crops associated with an imbalance between reactive oxygen species (ROS) and antioxidant defense systems. This study investigated the role of exogenous trehalose in mitigating CI by modulating ROS and antioxidant activities in ‘Kim Ju’ guava during low temperature storage (8 °C for 14 days) followed by shelf storage (25 °C for 4 days). The findings revealed a progressive rise in CI index and ROS level, encompassing superoxide radical and hydrogen peroxide (H2O2), in untreated fruits throughout the storage duration. Concurrently, the total antioxidant capacity, activities of enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione peroxidase (GPX), along with the redox status of non-enzymatic antioxidants, including the ratios of ascorbic acid/dehydroascorbate (ASA/DHA) and reduced glutathione/oxidized glutathione (GSH/GSSG), exhibited a declining trend during storage, except activity of peroxidase (POX). Trehalose treatment (immersion in 200 mM for 30 min) effectively reduced CI throughout the storage period. This reduction was associated with the upregulation of total antioxidant capacity, SOD, CAT, APX and GPX activities, along with increased total phenolic content and elevated ratios of ASA/DHA and GSH/GSSG, compared to untreated fruits. Additionally, POX activity and H2O2 level were significantly lower in trehalose-treated fruits. The results of this study indicate that low temperature stress causes an increase in ROS level and a decrease in enzymatic and non-enzymatic Chiang Mai Journal of Science 2 http://epg.science.cmu.ac.th/ejournal/ Chiang Mai Journal of Science Chiang Mai J. Sci., 2025; 52(2): e2025015 DOI 10.12982/CMJS.2025.015 antioxidant activities resulting in oxidative stress and leading to CI development in control fruit. On the other hand, application of trehalose can upregulate total antioxidant capacity including enzymatic and non-enzymatic antioxidant activities to overcome ROS leading to mitigating CI guava fruit.

Graphical Abstract:
Article ID
e2025015
Received Date 
2024-05-10
Revised Date 
2025-01-19
Accepted Date 
2025-02-24
Keyword 
antioxidant enzyme activity, reactive oxygen species, guava fruit, chilling injury, trehalose
Volume 
Vol.52 No.2 In progress (March 2025). This issue is in progress but contains articles that are final and fully citable.
DOI 
10.12982/CMJS.2025.015
Citation 
Vichaiya T., Chotikakham S. and Intarasit S., Regulation of Reactive Oxygen Species Metabolism by Trehalose Application Related to Alleviate Chilling Injury Development in Harvested ‘Kim Ju’ Guava Fruit, Chiang Mai Journal of Science, 2025; 52(2): e2025015. DOI 25.015.
SDGs
View:63 Download:0

  RELATED ARTICLE

Cytoprotection Against Oxidative Damage by Bioaccessible Fraction of Unripe Musa balbisiana Fruit Extract in An Intestinal-like Epithelial Cell Model
Article ID: e2024081
Author:Areeya Tohteb, Thammarat Kaewmanee, Piyawan Boonyanuphong and Tanyarath Utaipan
Vol.51 No.5 (September 2024) View: 411 Download:268
Reactive Oxygen Species Scavenging Enzyme Activities in Berangan Banana Plant Infected by Fusarium oxysporum f. sp. cubense
page: 1084 - 1095
Author:Fung Shi Ming, Zuliana Razali and Chandran Somasundram*
Vol.46 No.6 (November 2019) View: 1,725 Download:400
Ascorbic Acid Application Improves Salinity Stress Tolerance in Wheat
page: 1296 - 1306
Author:Manzer H Siddiqui*, Saud A Alamri, Mutahhar YY Al-Khaishany, Mohammed A Al-Qutami and Hayssam M Ali
Vol.45 No.3 (May 2018) View: 651 Download:295



Search in this journal


Document Search


Author Search

A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z

Popular Search






Chiang Mai Journal of Science

Faculty of Science, Chiang Mai University
239 Huaykaew Road, Tumbol Suthep, Amphur Muang, Chiang Mai 50200 THAILAND
Tel: +6653-943-467




Faculty of Science,
Chiang Mai University




EMAIL
cmjs@cmu.ac.th




Copyrights © Since 2021 All Rights Reserved by Chiang Mai Journal of Science