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Determination of the Effects of Adding Milk and Sugar on the Antioxidant Capacity of Oolong Tea by Chemical and Cell Culture-Based Antioxidant Assays


Paper Type 
Contributed Paper
Title 
Determination of the Effects of Adding Milk and Sugar on the Antioxidant Capacity of Oolong Tea by Chemical and Cell Culture-Based Antioxidant Assays
Author 
Angkana Wipatanawin, Sudarat Phongsawanit, Thadthanan Maneeratprasert, Sittiwat Lertsiri
Email 
angkana.s@cmu.ac.th ; s.angkana@gmail.com
Abstract:

 The effect of sugars and/or milk on the antioxidant capacity of Oolong tea was evaluated using 2,2´-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS), ferrous ion-chelating (FIC) and cellular antioxidant activity (CAA) assays. The addition of sugar did not alter the antioxidant capacity of tea (p < 0.05), however with the addition of sweetener an 18% and 14% increase was observed using ABTS and FIC assays respectively. In contrast, using the CAA method, the sweetener decreased the tea antioxidant capacity by 8%. Milks and milks and sugars decreased the antioxidant capacity of tea by 30% and 45% respectively determined by ABTS and CAA assays, with an opposite result using the FIC method.  The chemical and cellular based methods showed poorly correlated values.

 

Start & End Page 
699 - 711
Received Date 
2014-07-22
Revised Date 
Accepted Date 
2014-10-27
Full Text 
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Keyword 
tea, milk, sugar, sweetener, cellular antioxidant activity, FIC, ABTS, CAA
Volume 
Vol.42 No.3 (JULY 2015)
DOI 
SDGs
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Chiang Mai Journal of Science

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