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Production of Polyhydroxybutyrate (PHB) by Novel Bacillus megaterium SWU01 Isolated from Activated Sludge


Paper Type 
Contributed Paper
Title 
Production of Polyhydroxybutyrate (PHB) by Novel Bacillus megaterium SWU01 Isolated from Activated Sludge
Author 
Wisan Chuavong, Sirikwan Ponprateep, Pravech Ajawatanawong and Tipachai Vatanavicharn
Email 
tipachai.va@kmitl.ac.th
Abstract:

     Polyhydroxybutyrate (PHB) is a natural polymer accumulated in granules as intracellular energy storage in stress condition. The aim of this research was to isolate potential PHB producing bacteria from canned tuna wastewater activated sludge and cow dung. Five positive bacterial isolates were confirmed the accumulation of PHB using lipophilic staining and TEM. To identify the bacterial species, the phylogenetic analyses inferred from 16S rDNA gene sequence demonstrated that the bacterial strains were closely related to Bacillus genera. The comparison of PHB production from bacterial isolates showed that SWU01 strain isolated from the activated sludge exhibited the highest yield of PHB production. The cultivation of SWU01 stain in optimization condition (modified M9 medium supplemented with 2% sodium acetate and 0.5% yeast extract at pH 7 for 48 h) exhibited the yield of PHB up to 0.95 g/L or 7.5 compared with M9 medium supplemented with 2% glucose. Transmission electron micrograph showed high among of PHB in the granule of the bacterial cell. From all results, the bacterial strain SWU01, efficient PHB producers, exhibited the potential for their utilization in commercial PHB production.

Start & End Page 
1483 - 1499
Received Date 
2022-08-17
Revised Date 
2022-10-20
Accepted Date 
2022-11-14
Full Text 
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Keyword 
bioplastics, Bacillus spp., identification, PHB producing bacteria, polyhydroxybutyrate
Volume 
Vol.49 No.6 (November 2022)
DOI 
https://doi.org/10.12982/CMJS.2022.109
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Chiang Mai Journal of Science

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