Endophytic Fungi from Root of Three Lady’s Slipper Orchids (Paphiopedilum spp.) in Southern Thailand

Pornsawan Sutthinon; Kanin Rungwattana; Chalermpol Suwanphakdee; Winanda Himaman; Piyangkun Lueangjaroenkit

Endophytic Fungi from Root of Three Lady’s Slipper Orchids (Paphiopedilum spp.) in Southern Thailand

Pornsawan Sutthinon, Kanin Rungwattana, Chalermpol Suwanphakdee, Winanda Himaman and Piyangkun Lueangjaroenkit
* Author for corresponding; e-mail address: fscipkl@ku.ac.th
Volume: Vol.48 No.3 (Special Issue I : May 2021)
Research Article
DOI:
Received: 30 November 2020, Revised: -, Accepted: 17 April 2021, Published: -

Citation: Sutthinon P., Rungwattana K., Suwanphakdee C., Himaman W. and Lueangjaroenkit P., Endophytic Fungi from Root of Three Lady’s Slipper Orchids (Paphiopedilum spp.) in Southern Thailand, Chiang Mai Journal of Science, 2021; 48(3): 853-866.

Abstract

The life cycle of Paphiopedilum, the lady’s slipper orchid, under natural conditions requires fungal symbiosis, especially during the stage of seed germination. For orchid propagation and conservation, endophytic fungal isolation and orchid root anatomical observation are fundamental stages for mycorrhizal studies. Here, endophytic fungi were isolated from the root of three endemic orchids from Southern Thailand; Paphiopedilum niveum, P. exul and P. godefroyae. Fungal identification was based on morphological and molecular data. Results showed that all orchids exhibited several root anatomical adaptations. Endophytic fungal colonisation in their roots consisted of peloton structures in root cells of the cortex layer. Seven endophytic fungi belonging to four different taxa including Tulasnella sp., Fusarium solani, Phomopsis sp. and Daldinia eschscholtzii were identified from three orchid species. The genus Tulasnella is the most common endophytic fungi, forming root associations in several terrestrial orchids. Endophytic fungi can be applied for orchid propagation for successful future conservation.

Keywords: fungi, fungal identification, terrestrial orchid, peloton, anatomy

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