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In Vitro Symbiotic Seed Germination of Epipactis flava (Orchidaceae) Promoted by Endophytic Fungus, Tulasnella phuhinrongklaensis


Paper Type 
Contributed Paper
Title 
In Vitro Symbiotic Seed Germination of Epipactis flava (Orchidaceae) Promoted by Endophytic Fungus, Tulasnella phuhinrongklaensis
Author 
Nakarin Suwannarach, Jaturong Kumla, Chanalug Rachanarin and Kanok-Orn Srimuang
Email 
suwan.462@gmail.com
Abstract:

The rheophytic orchid, Epipactis flava, is a rare and endangered terrestrial orchid that grows

throughout Southeast Asia. In nature, seed germination and protocrom development of this orchid
species require a symbiotic association with fungi. In this study, Tulasnella phuhinrongklaensis CMUCR41,
was investigated for its compatibility to act as a symbiont during in vitro seed germination and
the protocorm development of this orchid. Percentage of symbiotic seed germination and protocorm
developmental rate index of the symbiotic treatment were higher than asymbiotic seed germination
treatment. The level of protocorm development was the most advanced at up to stage 3 over a course
of 65 days. The formation of peloton-like structures was found in the rhizoids and cortical cells of
protocorms in the symbiotic treatment. The identification of the fungal association was confirmed
through molecular methods. This is the first report on the in vitro seed germination and protocorm
development of E. flava using through the use of a compatible fungal symbiont.
Start & End Page 
787 - 792
Received Date 
2020-12-21
Revised Date 
Accepted Date 
2021-03-10
Full Text 
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Keyword 
fungal symbiont, orchid mycorrhizal fungi, rheophytic orchid, protocorm development
Volume 
Vol.48 No.3 (Special Issue I : May 2021)
DOI 
Citation 
Suwannarach N., Kumla J., Rachanarin C. and Srimuang K., In Vitro Symbiotic Seed Germination of Epipactis flava (Orchidaceae) Promoted by Endophytic Fungus, Tulasnella phuhinrongklaensis, Chiang Mai J. Sci., 2021; 48(3): 787-792.
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Chiang Mai Journal of Science

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