Paper Type |
Contributed Paper |
Title |
Antimicrobial Potential of Fungi Isolated from Soils of Dry Dipterocarp Forest in Northeast Thailand |
Author |
Panjamaphon Chanthasena, Watsana Penkhrue, Phimpha Khowangklang, Pishyaporn Sritangos and Nawarat Nantapong |
Email |
nawarat@sut.ac.th |
Abstract: A total of sixteen antimicrobial-producing fungal isolates were isolated from forest soil collected from Suranaree University of Technology (SUT), Thailand. These fungal isolates exhibited
antimicrobial activity against test pathogenic Gram-positive bacteria (methicillin-resistant Staphylococcus
aureus (MRSA), S. aureus, S. epidermidis, Bacillus subtilis, and B. cereus), Gram-negative bacteria (Escherichia
coli, Enterobacter aerogenes, Salmonella typhimurium, and Proteus mirabilis), and yeasts (Candida albicans,
C. tropicalis, and Saccharomyces cerevisiae). The fungal isolates that exhibited the highest antimicrobial
activity against MRSA, S. aureus, S. epidermidis, B. subtilis, B. cereus, E. coli, En. aerogenes, Sal. typhimurium,
P. mirabilis, C. albicans and C. tropicalis are PKF125, PKF60, PKF125, PKF6, PKF127, PKF104,
PKF152, PKF152, PKF152, PKF105 and PKF105, respectively. The highest antifungal activity toward
Sac. cerevisiae was observed from PKF77 and PKF105. An initial molecular classification of fungal strains
was conducted by sequencing the internal transcribed spacer (ITS) region. The ITS region of fungal
isolates were analyzed by BLAST search and phylogenetic analysis. The BLAST search revealed that
16 fungal isolates were members of the genus Aspergillus (9 isolates), Penicillium (3 isolates), Clonostachys
(2 isolates) and Talaromyces (2 isolates). The obtained fungal isolates in the genus Aspergillus were
separated into three sections including Flavipedes (PKF116, PKF145, and PKF105), Nigri (PKF161),
and Flavi (PKF59, PKF38, PKF6, PKF60, and PKF61). On the otherhand, isolates PKF121, PKF125,
and PKF127 were placed in Penicillium section Citrina. Isolate PKF152 and PKF124 were assigned to
Talaromyces section Talaromyces and Islandici, respectively. Isolate PKF77 and PKF104 were deemed as
Clonostachys rogersoniana. The current study suggests that unexplored forest soil represents a rich reservoir
for the search of new antimicrobial compounds. Fungal isolates in this study exhibited the ability to
inhibit MRSA, providing insights into pharmaceutical targeting against drug-resistant microorganisms |
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Start & End Page |
793 - 807 |
Received Date |
2020-12-13 |
Revised Date |
|
Accepted Date |
2021-03-24 |
Full Text |
Download |
Keyword |
antimicrobial activity, antimicrobial-producing fungi, drug-resistant microorganisms, MRSA, soil fungi |
Volume |
Vol.48 No.3 (Special Issue I : May 2021) |
DOI |
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SDGs |
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