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Biofuel Production, Characterization and Degradation of 3-hydroxybutyate Methyl Ester from Polyhydroxybutyrate


Paper Type 
 Contributed Paper
Title 
 Biofuel Production, Characterization and Degradation of 3-hydroxybutyate Methyl Ester from Polyhydroxybutyrate
Author 
 Kanokphorn Sangkharak*[a], Nisa Pichid [a], Tewan Yunu[a], Kamonthip Srinak [a], Sasithorn Sornnum [a] and Poonsuk Prasertsan [b]
Email 
 skanokphorn@yahoo.com
Abstract:
Poly(3-hydroxybutyrate) [P(3HB)], isolated from Bacillus licheniformis M2-12, were utilized as biofuel substrate. P(3HB) was esterified by acid- and alkali-catalysis to become 3-hydroxybutyrate methyl ester (3HBME). High 3HBME recovery percentage (68%) and purity (>95%) were obtained from acid catalytic processes. Afterward, 3HBME was further characterized for basic physical, chemical and fuel related properties. According to 3HBME properties such as density, flash point, boiling point and freezing point, 3HBME showed the possible ability to be utilized as a novel biofuel or a fuel additive. In addition, the fuel properties were also compared with ethanol, gasoline, diesel and biodiesel. The degradation of 3HBME was investigated under different storage conditions. Only 5% degradation was found when 3HBME was stored under 4°C. However, 3HBME degradation increased (30-90%) when the storage temperature increased (30-40°C). The highest rate of degradation (90%) was observed in samples stored under 40°C together with air exposure and water. 
Start & End Page 
 808 - 817
Received Date 
 2015-09-21
Revised Date 
Accepted Date 
 2015-11-22
Full Text 
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Keyword 
 Bacillus licheniformis, methyl ester, PHA, PHB, polyhydroxyalkanote, polyhydroxybutyrate
Volume 
 Vol.43 No.4 (JULY 2016)
DOI 
Citation 
 Sangkharak*[a] K., [a] N.P., Yunu[a] T., [a] K.S., [a] S.S. and [b] P.P., Biofuel Production, Characterization and Degradation of 3-hydroxybutyate Methyl Ester from Polyhydroxybutyrate , Chiang Mai Journal of Science, 2016; 43(4): 808-817.
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Author:Kanokphorn Sangkharak *[a], Piyaporn Wangsirikul [a,b], Nisa Pichid [a], Tewan Yunu [a] and Poonsuk
Vol.43 No.4 (JULY 2016) View: 697 Download:285
1, 3-Propanediol Production from Crude Glycerol by Klebsiella pneumoniae
page: 718 - 725
Author:Jiaxin Wu*[a], Fenghuan Wang[b], Zheng Wang[b], Hua Ye[c] and Peiyi Liu[d]
Vol.43 No.4 (JULY 2016) View: 704 Download:249
Production of Talaromyces (Penicillium) marneffei Glyceraldehyde-3-Phosphate Dehydrogenase Recombinant Protein Expressed by Pichia pastoris
page: 470 - 476
Author:Sophit Khanthawong* and Nongnuch Vanittanakom
Vol.43 No.3 (APRIL 2016) View: 675 Download:242
Sintered Dual-phase Steels Produced from Pre-alloyed Fe-Cr-Mo Powder
page: 358 - 364
Author:Wananurat Srijampan [a], Monnapas Morakotjinda [b], Rungtip Krataitong [b], Thanyaporn Yotkaew [b], Nattaya Tosangthum [b], Amporn Wiengmoon [a] and Ruangdaj Tongsri *[b]
Vol.43 No.2 (SPECIAL ISSUE 1) View: 695 Download:327
Earthy-musty Odour and Off-flavour Taints in Phayao Lake, Thailand
page: 22 - 31
Author:Supannee Suwanpakdee, Redel Gutierrez, Santiwat Pithakpol, Arunothai Jampeetong, Wasu Pathom-aree, Nakao Nomura, Tomoaki Itayama[f] and Niwooti Whangchai
Vol.43 No.1 (JANUARY 2016) View: 781 Download:346
Comparison of Some Modern IT Equipment as Detectors for Microfluidic Paper-based Determination of Phosphate
page: 176 - 182
Author:Wasin Wongwilai [a], B. Manori Jayawardane [b], Spas D. Kolev [b], Kate Grudpan [a] and Ian D. McKelvie*[b,c]
Vol.43 No.1 (JANUARY 2016) View: 672 Download:219
Assessment of genetic diversity of Jatropha curcas L. using AFLP and ISSR markers
page: 614 - 625
Author:Sirisak Soonthornyatara, Prapa Sripichitt, Rugsarit Kaveeta and Vipa Hongtrakul
Vol.42 No.3 (JULY 2015) View: 1,064 Download:241
Production and Immobilization of Levansucrase
page: 44 - 51
Author:Surawut Sangmanee, Santhana Nakapong, Kamontip Kuttiyawong and Rath Pichyangkura
Vol.42 No.1 (JANUARY 2015) View: 721 Download:237
Phylogenetic Study of the Botryosphaeriaceae Species Associated with Avocado and Pará rubber in Thailand.
page: 104 - 116
Author:Trakunyingcharoen T., Cheewangkoon R. and To-anun C.
Vol.42 No.1 (JANUARY 2015) View: 1,287 Download:264
Genetic Relationships among Indonesian Jatropha curcas L. Accessions, Selected Crossings, and Seed Oil Yield of Their Progenies
page: 1109 - 1120
Author:Andi Wijayaa, b, Memen Surahmanc, S. Susantidianad, Benyamin Lakitana,e
Vol.41 No.5/1 (OCTOBER 2014) View: 725 Download:268
Culturable Bacterial Community in a High Temperature EBPR Reactor
page: 970 - 980
Author:Ying Hui Ong 1a, Adeline Seak May Chua 2a*, Geok Yuan Annie Tan3b, and Gek Cheng Ngoh 4a
Vol.41 No.5/1 (OCTOBER 2014) View: 742 Download:244
Production of a rabbit polyclonal anti-elephant IgG antibody for the elephant laboratory investigation
page: 376 - 385
Author:Preeyanat Vongchan*
Vol.40 No.3 (JULY 2013) View: 720 Download:282
Co-precipitation of Mefenamic acid and Polyethylene Glycol 4000 Using the Gas Anti-Solvent (GAS) Process
page: 440 - 446
Author:Manop Charoenchaitrakool *[a, b], Siwaporn Suttikornchai [a, b] and Thitima Songjitsomboon [a]
Vol.40 No.3 (JULY 2013) View: 691 Download:226
Phytoplankton as a Bio-indicator of Water Quality in the Freshwater Fishing Area of Pak Phanang River Basin (Southern Thailand)
page: 344 - 355
Author:Amphorn Sakset* [a] and Wanninee Chankaew* [b]
Vol.40 No.3 (JULY 2013) View: 708 Download:356
Micellar Liquid Chromatographic Determination of Sildenafil Citrate in Pharmaceutical Formulations
page: 408 - 418
Author:Sujaritpun Jaipang [a], Dammrong Santiarworn [a], Saisunee Liawruangrath [b] and Boonsom Liawruangra
Vol.40 No.3 (JULY 2013) View: 658 Download:247
Effects of Calcination Conditions on Phase Formation and Particle Size of Zn2Nb34O87 Powders Synthesized by Solid-State Reaction
page: 135 - 142
Author:Penphitcha Amonpattaratkit and Supon Ananta
Vol.40 No.1 (JANUARY 2013) View: 628 Download:241
Characteristics of Phosphate Solubilization by Phosphate-Solubilizing Bacteria Isolated from Agricultural Chili Soil and Their Efficiency on the Growth of Chili (Capsicum frutescens L. cv. Hua Rua)
page: 11 - 25
Author:Worapon Surapat[a], Charida Pukahuta[a], Pongsak Rattanachaikunsopon[a], Tadanori Aimi[b], and Sopho
Vol.40 No.1 (JANUARY 2013) View: 700 Download:268
Post-treatment by Sodium Silicate after Zinc Phosphating on Cold-Rolled Steel
page: 30 - 40
Author:Supunsa Dech-oup, Sirinee Thaiwatthana and Torranin Chairaungsri
Vol.39 No.1 (JANUARY 2012) View: 683 Download:262
An Orphan Crop (Cucumeropsis mannii Naudin) with Interesting Phosphatase, β-galactosidase and α-mannosidase Activities: Nutritional Approach and Biochemical Characterization
page: 210 - 222
Author:Djary M. Koffi [a], Betty M. Faulet [b], Jean T. Gonnety [b], Mica l E. B dikou [a], Iri A. Zoro Bi
Vol.38 No.2 (APRIL 2011) View: 695 Download:223
Isothermal Phase Transformation Sequence in Fe-22wt%Cr-3.2wt%Mo-6.2wt%Ni-0.037wt%C Cast Duplex Stainless Steel
page: 47 - 55
Author:Surasak Kuimalee*[a], John T.H. Pearce [b] and Torranin Chairuangsri**[c]
Vol.38 No.1 (JANUARY 2011) View: 701 Download:233
A Direct Current Polarographic Method for the Determination of Chlorpheniramine Maleate in Pharmaceutical Preparations
page: 135 - 142
Author:Teraboon Pojanagaroon, Saisunee Liawruangrath, and Boonsom Liawruangrath
Vol.34 No.1 (JANUARY 2007) View: 684 Download:453
Dirac Equation : Wave Function and Phase Shifts for a Linear Potential
page: 15 - 22
Author:Lalit K. Sharma, and Lesolle D. Sebitla
Vol.34 No.1 (JANUARY 2007) View: 642 Download:236
Preliminary Study of Anthraquinone in Sweet Bamboo (Dendrocalamus asper Backer) Alkaline Sulfite Pulping
page: 235 - 247
Author:Suphat Kamthai
Vol.34 No.2 (MAY 2007) View: 1,243 Download:1,491
Optimization Method for Determination of Carbofuran and Carboxin Residues in Cabbages by SPE and HPLC-UV
page: 227 - 234
Author:Khin L. Zan, and Somporn Chantara
Vol.34 No.2 (MAY 2007) View: 692 Download:257
Finite Element Solution for 1-D Groundwater Flow, Advection-Dispersion, and Interphase Mass Transfer: II. Experimental Validation
page: 161 - 168
Author:Satawat Saenton*[a] and Morrakot Khebchareon [b]
Vol.33 No.2 (MAY 2006) View: 686 Download:177
High Performance Thin Layer Chromatographic Method for the Determination of Diclofenac Sodium in Pharmaceutical Formulations
page: 123 - 128
Author:Wisanu Thongchai, Boonsom Liawruangrath*, Chalermporn Thongpoon and Theeraphan Machan
Vol.33 No.1 (JANUARY 2006) View: 818 Download:229
Pressure-Temperature Estimates of the Metamorphosed Sedimentary Rocks on the Eastern Side of Khao Phai, Immediate West of Ban Choeng Chai, Wang Pong, Phetchabun Province, Thailand
page: 283 - 293
Author:Yuenyong Panjasawatwong, Boontarika Srithai and Udomporn Wipakul
Vol.35 No.2 (MAY 2008) View: 710 Download:215
Novel Elastic Nanovesicles for Cosmeceutical and Pharmaceutical Applications
page: 168 - 178
Author:Aranya Manosroi, Pensak Jantrawut, Narinthorn Khositsuntiwong, Worapaka Manosroi, and Jiradej Manosr
Vol.36 No.2 (MAY 2009) View: 702 Download:245
Characterization of Perovskite Lead Iron Niobate Powders Synthesized by Partial Oxalate
page: 71 - 76
Author:Pitak Youmee [a] and Sukon Phanichphant* [b]
Vol.32 No.1 (JANUARY 2005) View: 1,128 Download:190
Stability Study of Six 1,4-Benzodiazepines in Bio-fluids Stored at -20oC
page: 451 - 463
Author:Mohammad N. Uddin, Victoria F. Samanidou, and Ioannis N. Papadoyannis
Vol.37 No.3 (SEPTEMBER 2010) View: 812 Download:391
Retention Behavior of Aromatic Amines with Some Ionic Liquids Mobile Phase in Semi-micro HPLC
page: 384 - 396
Author:Yuppadee Nusai, Hitoshi Koizumi, Masaki Tachibana, Kazue Tani, and Nobutoshi Kiba
Vol.37 No.3 (SEPTEMBER 2010) View: 756 Download:231



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