Culturable Bacterial Community in a High Temperature EBPR Reactor
Ying Hui Ong 1a, Adeline Seak May Chua 2a*, Geok Yuan Annie Tan3b, and Gek Cheng Ngoh 4a* Author for corresponding; e-mail address: ngoh@um.edu.my
Volume: Vol.41 No.5/1 (OCTOBER 2014)
Research Article
DOI:
Received: 25 June 2013, Revised: -, Accepted: 8 January 2014, Published: -
Citation: 1a Y.H.O., 2a A.S.M.C., Tan3b G.Y.A. and 4a G.C.N., Culturable Bacterial Community in a High Temperature EBPR Reactor, Chiang Mai Journal of Science, 2014; 41(5/1): 970-980.
Abstract
An enhanced biological phosphorus removal (EBPR) reactor operated at high temperature (28oC) exhibited good phosphorus removal efficiencies of 99% throughout a 2-year operation. To investigate the culturable bacterial community of this system, isolation of bacteria was carried out on agar media containing nutrients similar to that in the EBPR reactor. Analyses of 16S rRNA gene sequences of selected bacterial isolates were found to belong to α-Proteobacteria, β-Proteobacteria, γ-Proteobacteria, Flavobacteria, Bacilli, Sphingobacteria and Actinobacteria. The dominant community was β-Proteobacteria which included Acidovorax spp., Achromobacter spp., and Hydrogenophaga spp. Out of the 24 species identified, some of the bacterial strains were not commonly found in previously reported EBPR processes. Thus, this has contributed to the uniqueness of microbial population in EBPR process operated at high temperature.