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Effect of Temperature on Schizophyllum commune Growth and 4H-pyran-4-one,2,3-dihydro-3, 5-dihydroxy-6-methyl- Production using a Bubble Column Bioreactor


Paper Type 
 Contributed Paper
Title 
 Effect of Temperature on Schizophyllum commune Growth and 4H-pyran-4-one,2,3-dihydro-3, 5-dihydroxy-6-methyl- Production using a Bubble Column Bioreactor
Author 
 Yi Peng Teoh and Mashitah Mat Don*
Email 
 chmashitah@usm.my
Abstract:
 Schizophyllum commune, a basidiomycetes species, exhibited the anti-fungal properties by producing several bioactive compounds during secondary metabolism. One flavonoid fraction, named 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- (DDMP), has received much attention for its antifungal activity toward wood-degrading fungi of rubberwood. In the present study, the effect of temperature on the growth and bioactive compound production by Schizophyllum commune was investigated in a bubble column bioreactor. Results revealed that the highest production of biomass (35.11±0.12 g/L), total flavonoids (1.328±0.007 µg QE/mg sample), and DDMP (1.278±0.005 µg/mg sample) were achieved in the culture grown at 30 oC. The separation of crude extract was studied using a high performance liquid chromatography (HPLC) system equipped with a Zorbax XDB C18 column.

Start & End Page 
 462 - 469
Received Date 
 2014-02-18
Revised Date 
Accepted Date 
 2014-06-28
Full Text 
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Keyword 
 Schizophyllum commune, Total flavonoid, 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- (DDMP), Bubble column bioreactor, Temperature, High performance liquid chromatography (HPLC)
Volume 
 Vol.43 No.3 (APRIL 2016)
DOI 
Citation 
 Teoh Y.P. and Don M.M., Effect of Temperature on Schizophyllum commune Growth and 4H-pyran-4-one,2,3-dihydro-3, 5-dihydroxy-6-methyl- Production using a Bubble Column Bioreactor, Chiang Mai J. Sci., 2016; 43(3): 462-469.
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