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Effect of temperature on Schizophyllum commune growth and 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- production using a bubble column bioreactor
Paper Type  |
Contributed Paper |
Title |
Effect of temperature on Schizophyllum commune growth and 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- production using a bubble column bioreactor |
Author |
Yi Peng, Teoh and Mashitah, Mat Don |
Email |
chmashitah@usm.my |
|
Abstract: Schizophyllum commune, a basidiomycetes species, exhibited the anti-fungal properties by producing several bioactive compounds during secondary metabolism. One flavonoid fraction, named 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- (DDMP), has received much attention for its antifungal activity toward wood-degrading fungi of rubberwood. In the present study, the effect of temperature on the growth and bioactive compound production by Schizophyllum commune was investigated in a bubble column bioreactor. Results revealed that the highest production of biomass (35.11±0.12 g/L), total flavonoids (1.328±0.007 µg QE/mg sample), and DDMP (1.278±0.005 µg/mg sample) were achieved in the culture grown at 30 oC. The separation of crude extract was studied using a high performance liquid chromatography (HPLC) system equipped with a Zorbax XDB C18 column. |
|
Start & End Page |
539 - 548 |
Received Date |
2014-02-18 |
Revised Date |
|
Accepted Date |
2014-07-28 |
Full Text |
Download |
Keyword |
Schizophyllum commune, Total flavonoid, 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- (DDMP), Bubble column bioreactor, Temperature, High performance liquid chromatography (HPLC) |
Volume |
Vol.42 No.3 (JULY 2015) |
DOI |
|
Citation |
Peng Y., Teoh , Mashitah and Don M., Effect of temperature on Schizophyllum commune growth and 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- production using a bubble column bioreactor , Chiang Mai Journal of Science, 2015; 42(3): 539-548. |
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