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Effect of temperature on Schizophyllum commune growth and 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- production using a bubble column bioreactor


Paper Type 
Contributed Paper
Title 
Effect of temperature on Schizophyllum commune growth and 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- production using a bubble column bioreactor
Author 
Yi Peng, Teoh and Mashitah, Mat Don
Email 
chmashitah@usm.my
Abstract:

 Schizophyllum commune, a basidiomycetes species, exhibited the anti-fungal properties by producing several bioactive compounds during secondary metabolism. One flavonoid fraction, named 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- (DDMP), has received much attention for its antifungal activity toward wood-degrading fungi of rubberwood. In the present study, the effect of temperature on the growth and bioactive compound production by Schizophyllum commune was investigated in a bubble column bioreactor. Results revealed that the highest production of biomass (35.11±0.12 g/L), total flavonoids (1.328±0.007 µg QE/mg sample), and DDMP (1.278±0.005 µg/mg sample) were achieved in the culture grown at 30 oC. The separation of crude extract was studied using a high performance liquid chromatography (HPLC) system equipped with a Zorbax XDB C18 column.

Start & End Page 
539 - 548
Received Date 
2014-02-18
Revised Date 
Accepted Date 
2014-07-28
Full Text 
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Keyword 
Schizophyllum commune, Total flavonoid, 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- (DDMP), Bubble column bioreactor, Temperature, High performance liquid chromatography (HPLC)
Volume 
Vol.42 No.3 (JULY 2015)
DOI 
Citation 
Peng Y., Teoh and Don M.M., Effect of temperature on Schizophyllum commune growth and 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- production using a bubble column bioreactor , Chiang Mai J. Sci., 2015; 42(3): 539-548.
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