Chiang Mai Journal of Science

     Water temperature is one of the most important effects on aquatic physiology and survival rate. The primordial germ cell (PGC) development in post-larvae stages was conducted to determine the effect of high-water temperature using the histology technique. This research was conducted by incubating white shrimp (Litopenaeus vannamei) post -larvae at 3, 4, 5, 11, 15 days (n = 3) with three different water temperatures (25, 34, and 37 °C). The gonad development was observed in the aspect of PGCs migration to the genital ridge by using Hematoxylin and Eosin staining (H&E). Among the PGCs of shrimp cultured in high water temperatures, they decreased in number and size when compared to the control group (25 °C). While the PCGs development of the post-larvae cultured at 34 and 37 °C were not significantly different in the number and size. Based on this result, the high temperature affected the reduction of PGCs number and their migration during the larvae stage. This study is useful as a guide to establishing fewer germ cells in white shrimp for future transplantation techniques.