Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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Selective Isolation of Sorbitol Utilizing Acetic Acid Bacteria

Jeerapun Jaiinphon, Kreuawan Thonglem and Wasu Pathom-aree*
* Author for corresponding; e-mail address: wasu_pathomaree@hotmail.com
Volume: Vol.29 No.3 (DECEMBER 2002)
Research Article
DOI:
Received: 5 March 2002, Revised: -, Accepted: 10 October 2002, Published: -

Citation: Jaiinphon J., Thonglem K. and Pathom-aree W., Selective Isolation of Sorbitol Utilizing Acetic Acid Bacteria, Chiang Mai Journal of Science, 2002; 29(3): 189-192.

Abstract

The selective isolation of sorbitol utilizing acetic acid bacteria using specific enrichment medium in combination with the incubation temperature of 37 °C was reported. The medium which composed of 0.5 % glucono-delta-lactone and 1% sorbitol has been used for the selective isolation of acetic acid bacteria from flowers, fruits and vegetables. A total of 48 acetic acid bacteria were successfully recovered from the samples. Among these isolates, 85% were sorbitol utilizing acetic acid bacteria which grew well at 37 °C. Morphological and biochemical determinations indicated that all isolates are member of the genus Gluconobacter.

Keywords: selective isolation, acetic acid bacteria, sorbitol utilizing, enrichment

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