Submit your paper 
Download Template JOURNAL DETAIL
Stability Study of Six 1,4-Benzodiazepines in Bio-fluids Stored at -20oC
Paper Type  |
Contributed Paper |
Title |
Stability Study of Six 1,4-Benzodiazepines in Bio-fluids Stored at -20oC |
Author |
Mohammad N. Uddin, Victoria F. Samanidou, and Ioannis N. Papadoyannis |
Email |
nasiru_cu@yahoo.com |
|
Abstract: An approach has been undertaken to study the stability of six 1,4-benzodiazepines; alprazolam, bromazepam, clonazepam, diazepam, flunitrazepam and lorazepam in spiked plasma, urine and saliva samples at defined time intervals. Quantification was carried out using high-performance liquid chromatography equipped with a photodiode-array ultraviolet detector while extraction was performed using SPE. Spiked samples (0.5, 2.0 ng μL-1) were stored at -20oC and analysed at selected times during 180 days for both plasma and urine, and 120 days for saliva. At -20 oC, at the end of the observation period decrease was measured around 40% for flunitraepam and clonazepam and around 20% for remaining four benzodiazepines of the original levels. At end of study period for low concentrations (0.5 ng μL-1) all benzodiazepines were though detectable but a clear pattern of breakdown could not be established. Results of stability study through freeze-thaw cycle during seven successive days for spiked samples stored at -20oC were also reported. All analytes were seemed to be completely stable up to five cycles in plasma, urine, and up to three cycles in saliva. Moreover, stability study of all analytes over a period of one year revealed that standard solution in methanol stored at 4oC can be used without any remarkable degradation. The data collected suggest that quantitative results concerning long-term stored samples should be interpreted with caution in forensic cases.
|
|
Start & End Page |
451 - 463 |
Received Date |
2009-07-22 |
Revised Date |
|
Accepted Date |
2010-05-03 |
Full Text |
Download |
Keyword |
1,4-benzodiazepines, bio-fluids, plasma, urine and saliva, solid-phase extraction |
Volume |
Vol.37 No.3 (SEPTEMBER 2010) |
DOI |
|
Citation |
Uddin M.N., Samanidou V.F., and Papadoyannis I.N., Stability Study of Six 1,4-Benzodiazepines in Bio-fluids Stored at -20oC , Chiang Mai Journal of Science, 2010; 37(3): 451-463. |
SDGs |
|
| View:770 Download:376 | |
RELATED ARTICLE
Transcriptome Study of Cold Plasma Treated Pseudomonas aeruginosa
Article ID: e2023014
Author:Kok Jun Liew, Xinhua Zhang†, Xiaohong Cai, Dongdong Ren, Wei Liu, Zhidong Chang and Chun Shiong Chong
Vol.50 No.2 (March 2023) View: 706 Download:425
Article ID: e2023014
Author:Kok Jun Liew, Xinhua Zhang†, Xiaohong Cai, Dongdong Ren, Wei Liu, Zhidong Chang and Chun Shiong Chong
Vol.50 No.2 (March 2023) View: 706 Download:425
Effects of Plasma Technique and Gamma Irradiation on Seed Germination and Seedling Growth of Chili Pepper
page: 73 - 82
Author:Monthida Thisawech, Orapin Saritnum, Sureeporn Sarapirom, Kittikhun Prakrajang and Wannausa Phakham
Vol.47 No.1 (January 2020) View: 1,333 Download:704
page: 73 - 82
Author:Monthida Thisawech, Orapin Saritnum, Sureeporn Sarapirom, Kittikhun Prakrajang and Wannausa Phakham
Vol.47 No.1 (January 2020) View: 1,333 Download:704
SDGs:
Hydroxyl Density Measurement by Two-Beam UV-LED Absorption Spectroscopy in an Atmospheric-Pressure
Ar-H2O2 Vapor HF Plasma Jet
page: 1015 - 1022
Author:Wasin Nupangtha, Athipong Ngamjarurojana, Mudtorlep Nisoa and Dheerawan Boonyawan
Vol.46 No.5 (September 2019) View: 728 Download:292
page: 1015 - 1022
Author:Wasin Nupangtha, Athipong Ngamjarurojana, Mudtorlep Nisoa and Dheerawan Boonyawan
Vol.46 No.5 (September 2019) View: 728 Download:292
Effect of Atmospheric Pressure Plasma Treatment on Seed Decontamination and Sprouting of Pak Choi (Brassica rapa L. subsp. chinensis (L.) Hanelt)
page: 2679 - 2690
Author:Pradeep Puligundla, Je-Wook Kim and Chulkyoon Mok
Vol.45 NO.7 (November 2018) View: 730 Download:243
page: 2679 - 2690
Author:Pradeep Puligundla, Je-Wook Kim and Chulkyoon Mok
Vol.45 NO.7 (November 2018) View: 730 Download:243
Effect of Acetylene Concentration on Structural Properties of Hydrogenated Amorphous Carbon Films Prepared using Showerhead Plasma Chemical Vapor Deposition
page: 2471 - 2480
Author:Artit Chingsungnoen, Thananchai Dasri, Phitsanu Poolcharuansin, Anthika Lakhonchai, Nongkhan Tidngim, Pitak Eiamchai, Noppadon Nuntawong and Vittaya Amornkitbamrung
Vol.45 No.6 (September 2018) View: 814 Download:1,069
page: 2471 - 2480
Author:Artit Chingsungnoen, Thananchai Dasri, Phitsanu Poolcharuansin, Anthika Lakhonchai, Nongkhan Tidngim, Pitak Eiamchai, Noppadon Nuntawong and Vittaya Amornkitbamrung
Vol.45 No.6 (September 2018) View: 814 Download:1,069
Simple ITO Surface Treatments Induced Better Performance for Low Cost Organic Solar Cells
page: 2178 - 2189
Author:Sarunchana Wiboonsak, Khathawut Lohawet, Binh Au Thanh Duong, Pisist Kumnorkaew and Wantana Koetniyom
Vol.45 No.5 (Special 2018) View: 694 Download:321
page: 2178 - 2189
Author:Sarunchana Wiboonsak, Khathawut Lohawet, Binh Au Thanh Duong, Pisist Kumnorkaew and Wantana Koetniyom
Vol.45 No.5 (Special 2018) View: 694 Download:321
In Vitro Cultivation of Porcine Chondrocytes on Fully Surface-Modified Polycaprolactone Scaffold:
Static versus Dynamic Cultures
page: 2092 - 2104
Author:Pakkanun Kaewkong, Paweena Uppanan, Wasana Kosorn, Marut Wongcumchang, Pronrumpa Kanjanasingh, Tareerat Lertwimol and Wanida Janvikul
Vol.45 No.5 (Special 2018) View: 704 Download:249
page: 2092 - 2104
Author:Pakkanun Kaewkong, Paweena Uppanan, Wasana Kosorn, Marut Wongcumchang, Pronrumpa Kanjanasingh, Tareerat Lertwimol and Wanida Janvikul
Vol.45 No.5 (Special 2018) View: 704 Download:249
Sterilization of Medical Equipment by Plasma Technology
page: 1811 - 1826
Author:Norrapon Vichiansan, Noppakarn Kaai, Komgrit Leksakul, Saisamorn Lumyong and Jeon Geon Han
Vol.45 No.4 (July 2018) View: 727 Download:370
page: 1811 - 1826
Author:Norrapon Vichiansan, Noppakarn Kaai, Komgrit Leksakul, Saisamorn Lumyong and Jeon Geon Han
Vol.45 No.4 (July 2018) View: 727 Download:370
Antioxidant Properties of Crocodylus siamensis Blood Components on H2O2-induced Human Skin Fibroblast Cells
page: 1359 - 1371
Author:Nisachon Jangpromma, Khantheeranut Suttee, Santi Phosri, Tinnakorn Theansungnoen, Jiraporn Lueangsakulthai, Wisarut Payoungkiattikun, Sakda Daduang and Sompong Klaynongsruang*
Vol.45 No.3 (May 2018) View: 796 Download:262
page: 1359 - 1371
Author:Nisachon Jangpromma, Khantheeranut Suttee, Santi Phosri, Tinnakorn Theansungnoen, Jiraporn Lueangsakulthai, Wisarut Payoungkiattikun, Sakda Daduang and Sompong Klaynongsruang*
Vol.45 No.3 (May 2018) View: 796 Download:262
Thermally Oxidized Olive Oil Produces Biochemical, Physiological Effects and Fatty Liver in Rats
page: 847 - 857
Author:Ayaz Ali Khan, Alam Zeb* and Syed Tufail Hussain Shirazi
Vol.44 No.3 (July 2017) View: 626 Download:266
page: 847 - 857
Author:Ayaz Ali Khan, Alam Zeb* and Syed Tufail Hussain Shirazi
Vol.44 No.3 (July 2017) View: 626 Download:266
Lipid Peroxidation of Escherichia coli by Triplet Non-thermal Atmospheric Pressure Plasma Jet
page: 678 - 687
Author:A. Hossienzadeh Colagar* [a,b], O. Alavi [a], F. Sohbatzadeh [b, c]
Vol.44 No.2 (April 2017) View: 663 Download:190
page: 678 - 687
Author:A. Hossienzadeh Colagar* [a,b], O. Alavi [a], F. Sohbatzadeh [b, c]
Vol.44 No.2 (April 2017) View: 663 Download:190
Effect of Plasma Power on Copper Substrate Used for Synthesizing Carbon Nanotubes via
Alcohol Catalytic Chemical Vapor Deposition (ACVD) Technique
page: 339 - 344
Author:Sureewan Phunwaree*, Wim Nhuapeng, Dheerawan Boonyawan, and Wandee Thamjaree*
Vol.43 No.2 (SPECIAL ISSUE 1) View: 674 Download:197
page: 339 - 344
Author:Sureewan Phunwaree*, Wim Nhuapeng, Dheerawan Boonyawan, and Wandee Thamjaree*
Vol.43 No.2 (SPECIAL ISSUE 1) View: 674 Download:197
Characterization of NiO–α-MoO3 Composites Synthesized by Microwave Plasma
page: 276 - 281
Author:Surin Promnopas, Orawan Wiranwetchayan, Titipun Thongtem, Wonchai Promnopas and Somchai Thongtem
Vol.43 No.2 (SPECIAL ISSUE 1) View: 647 Download:338
page: 276 - 281
Author:Surin Promnopas, Orawan Wiranwetchayan, Titipun Thongtem, Wonchai Promnopas and Somchai Thongtem
Vol.43 No.2 (SPECIAL ISSUE 1) View: 647 Download:338
Plasma Assisted Immobilization of TiO2 nanoparticles onto PLA Surface to Promote the Antimicrobial Activity
page: 1361 - 1374
Author:Chonlada Theerakarunwong*[a], Somruthai Tunma[b] and Dheerawan Boonyawan[c]
Vol.41 No.5/2 (OCTOBER 2014) View: 613 Download:202
page: 1361 - 1374
Author:Chonlada Theerakarunwong*[a], Somruthai Tunma[b] and Dheerawan Boonyawan[c]
Vol.41 No.5/2 (OCTOBER 2014) View: 613 Download:202
Some Microstructural Studies of Plasma-sprayed Stainless Steel 316L/Al2O3-TiO2 Produced Using Two-Powder Port Configuration
page: 798 - 803
Author:Phuangphaga Daram [a], Jirasak Sukunta [a], Chalermchai Sukhonket [b], Hathaipat Koiprasert [b] and
Vol.40 No.4 (OCTOBER 2013) View: 753 Download:207
page: 798 - 803
Author:Phuangphaga Daram [a], Jirasak Sukunta [a], Chalermchai Sukhonket [b], Hathaipat Koiprasert [b] and
Vol.40 No.4 (OCTOBER 2013) View: 753 Download:207
Self-Diffusion Coefficient of Liquid Alkali Metals
page: 125 - 129
Author:Sant K. Srivastava* [a] and Kishor G. Kolhe [b]
Vol.31 No.2 May 2004 View: 662 Download:163
page: 125 - 129
Author:Sant K. Srivastava* [a] and Kishor G. Kolhe [b]
Vol.31 No.2 May 2004 View: 662 Download:163
Theoretical Study of the Bombyx mori Silk Surface
Functionalization: Quantum Mechanical Calculation
of the Glycine-Alanine Unit Reacting with Fluorine
and Molecular Dynamic Simulation of Wettability
page: 106 - 115
Author:Padungsee Khomhoi, Waleepan Sangprasert, Vannajan S. Lee, and Piyarat Nimmanpipug
Vol.37 No.1 (JANUARY 2010) View: 655 Download:216
page: 106 - 115
Author:Padungsee Khomhoi, Waleepan Sangprasert, Vannajan S. Lee, and Piyarat Nimmanpipug
Vol.37 No.1 (JANUARY 2010) View: 655 Download:216
Copyrights © Since 2021 All Rights Reserved by Chiang Mai Journal of Science