Chiang Mai Journal of Science

Low-cost production of indole-3-acetic acid fermentation by Enterobacter sp.

Paper Type	Contributed Paper
Title	Low-cost production of indole-3-acetic acid fermentation by Enterobacter sp.
Author	Pumin Nutaratat and Nantana Srisuk*
Email	fscints@ku.ac.th
Abstract: Enterobacter sp. DMKU-RP206 is a plant growth-promoting bacterium that can promote plant growth through direct and indirect mechanisms. At the time of writing, the bacterium Enterobacter sp. DMKU-RP206 has been claimed as the best IAA producer among the members of the genus Enterobacter. However, the drawback with regard to IAA production from this bacterium is the high cost of the production medium. A major cost component is reagent grade precursor, L-tryptophan, which is an essential and very expensive part of the IAA production medium. Therefore, the present study investigated the scaled-up IAA production by Enterobacter sp. DMKU-RP206 using a low-cost precursor. This study showed that it is possible to successfully reduce production costs by 52-fold as a result of the substitution of reagent grade L-tryptophan with a low-cost L-tryptophan (feed grade). The use of the low-cost medium gave the highest IAA concentration of 3,340.8 mg/L (equivalent to an IAA productivity of 27.8 mg/L/h and IAA yield of 1.97 mg/mg L-tryptophan) when 10% bacterial inoculum was used.
Start & End Page	653 - 660
Received Date	2018-10-15
Revised Date
Accepted Date	2019-03-26
Full Text	Download
Keyword	Enterobacter sp., feed grade L-tryptophan, indole-3-acetic acid, low cost production, sweet whey
Volume	Vol.46 No.4 (July 2019)
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