Chiang Mai Journal of Science

Paper Type 

Title 

Author 

Email 

Seeds extract of Cucumeropsis mannii Naudin (Cucurbitaceae), an orphan and underresearched crop was screened for enzymatic hydrolytic activities over a variety of natural and synthetic substrates. Enzymatic activities noticed mainly consist of phosphatase (0.66 0.02 UI/mg), β-galactosidase (0.26 0.03 UI/mg) and α-mannosidase (0.19 0.02 UI/mg) activities. The higher phosphatase activity observed suggest that this edible seed constitutes a potential source of phosphorus for human and animal diet. Exploration of biochemical properties has revealed that phosphatase, β-galactosidase and α-mannosidase activities were maximal at 45oC (pH 5.6), 55oC (pH 4.6) and 60oC (pH 5.0), respectively. The three enzymatic activities displayed also interesting characteristics as relative pH stability, resistance to (or activation by) detergents and hydrolytic specificity over natural substrates such as ATP, ADP, lactose and mannobioses. So, these disclosed enzymatic activities should deserve main investigations for further biotechnological applications. Keywords: Orphan crop, Cucumeropsis mannii seed, cucurbitaceae, phosphatase, β-galactosidase, α-mannosidase.

Start & End Page 

Received Date 

Revised Date 

Accepted Date 

Full Text 

Keyword 

Volume 

DOI 

Citation 

SDGs