Chiang Mai Journal of Science

BMP-2 Incorporation into 3D Printed Porous Hydroxyapatite by Rapid Biomimetic Co-Precipitation Technique Using Accelerated Calcium Phosphate Solution

Paper Type	Contributed Paper
Title	BMP-2 Incorporation into 3D Printed Porous Hydroxyapatite by Rapid Biomimetic Co-Precipitation Technique Using Accelerated Calcium Phosphate Solution
Author	Faungchat Thammarakcharoen and Jintamai Suwanprateeb*
Email	jintamai@mtec.or.th
Abstract: Bone morphogenetic protein (BMP-2) is multi-functional growth factor and widely used osteoinductive cell mediator which was loaded into the osteoconductive bone graft to accelerate bone healing and osteointegration of the graft. Rapid biomimetic co-precipitation technique by using newly developed accelerated calcium phosphate solution was investigated as a mean to enhance the incorporation of BMP-2 on three dimensionally printed porous hydroxyapatite (3DP HA) and its release characteristic compared to typical direct adsorption. Two initial BMP-2 concentrations of 1 and 10 μg/mL were employed in this study. After loading, new octacalcium phosphate leaf-like crystals were observed to form on the surface of BMP-2 loaded 3DP HA by using biomimetic co-precipitation technique at both concentrations while none was seen in the direct adsorption technique. Biomimetic co-precipitation technique yielded two times greater in total BMP-2 loading content, but displayed lower releasing rate than those of the direct adsorption technique. Cell differentiation of pre-osteoblast MC3T3-E1 on the BMP-2 loaded 3DP HA by using biomimetic co-precipitation was also greater enhanced compared to non-loaded or direct adsorbed samples. The increase in content and decrease in releasing rate of BMP-2 by using biomimetic co-precipitation technique could be related to the formation of new octacalcium phosphate crystals on the 3DP HA which incorporated BMP-2 into their structures and retarded its release.
Start & End Page	723 - 737
Received Date	2019-07-25
Revised Date
Accepted Date	2019-12-03
Full Text	Download
Keyword	BMP-2, hydroxyapatite, calcium phosphate, co-precipitation, biomimetic
Volume	Vol.47 No.4 (Special Issue II : July 2020)
DOI
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