Bioactivity, Cytotoxicity and Antibacterial Evaluation of Undoped, Zn-doped, Sr-doped, and 
Zn/Sr-codoped Hydroxyapatites Synthesized by a Sol-gel Method

Likit Temprom [a]; Suphasinee L. Seet [a; b; c]; Patcharaporn Tippayawat  [d]; Pimsiree Suwanna [a; b

Bioactivity, Cytotoxicity and Antibacterial Evaluation of Undoped, Zn-doped, Sr-doped, and Zn/Sr-codoped Hydroxyapatites Synthesized by a Sol-gel Method

Likit Temprom [a], Suphasinee L. Seet [a,b,c], Patcharaporn Tippayawat [d], Pimsiree Suwanna* [a,b,
* Author for corresponding; e-mail address: pimsiree.s@ku.ac.th
Volume: Vol.44 No.2 (April 2017)
Research Article
DOI:
Received: 3 April 2015, Revised: -, Accepted: 13 October 2015, Published: -

Citation: Temprom L., Seet S.L., Tippayawat P., [a P.S. and B , Bioactivity, Cytotoxicity and Antibacterial Evaluation of Undoped, Zn-doped, Sr-doped, and Zn/Sr-codoped Hydroxyapatites Synthesized by a Sol-gel Method, Chiang Mai Journal of Science, 2017; 44(2): 630-639.

Abstract

Undoped, Zn-doped, Sr-doped and Zn/Sr-codoped hydroxyapatite nanoparticles were synthesized using a sol-gel method. Their phases, morphologies and particle sizes were analyzed by XRD, FTIR and TEM techniques. MTT assay was performed to test cell viability against these nanoparticles. Forming ability of bonelike apatite and antibacterial activities were evaluated by immersing them in simulated body fluid (SBF) and by using agar disc diffusion assay against gram-positive Staphylococcus epidermidis and gram-negative Pseudomonas aeruginosa, respectively. The XRD and FTIR results showed the characteristic peaks of the hydroxyapatite structure; whereas the TEM results revealed nanoparticles with the size of 40-300 nm. The MTT assay indicated these nanoparticles are nontoxic. In SBF, significantly higher amount of apatite was formed on the doped-HA than on the regular HA. Incorporation of Zn ion into HA was observed to stimulate agglomeration of the newly formed apatite, whereas incorporation of Sr ion seemed to spread out the small apatite granules. Antibacterial tests demonstrated that HA alone could only inhibit the viability of P. aeruginosa but not S. epidermidis. However, its antibacterial capability against both strains was enhanced by Sr and/or Zn doping, with Sr being more effective. These doped-HA nanoparticles, therefore, could be promising candidates for future biomedical applications with good antibacterial property and apatite forming ability.

Keywords: hydroxyapatite, bioactivity, biocompatibility, antibacterial

Red Onion Peels Extract: A Food Waste for Silver Nanoparticles Synthesis and Potential Application in Air Cleaning Devices
DOI: 10.12982/CMJS.2025.012.

Sakoolrud Raunmoon, Varintorn Bangwiset, Wanrudee Kaewmesri, Arthid Thim-uam, Paideang Khwanchai, Chee O. Too and Widsanusan Chartarrayawadee

Vol.52 No.2 (March 2025)
Research Article View: 1,108 Download: 307

Enhanced Photocatalytic Degradation of Selected Contaminant Dyes from Textile Sewage Discharge and Electrochemical Efficiency using Citrus limetta Mediated ZnO Nanopellets
DOI: 10.12982/CMJS.2024.106.

Sasi Florence

Vol.51 No.6 (November 2024)
Research Article View: 772 Download: 452

Synthesis and Characterization of β-Tricalcium Phosphate and Its Film Coating on a Ti6Al4V Metallic Biomaterial
DOI: 10.12982/CMJS.2024.086.

Waraporn Boontakam, Kamonporn Saenkam, Phanrawee Sriprapha, Komsanti Chokethawai, Chamnan Randorn, Nopakarn Chandet, Kriangkrai Thongkorn, Tawee Tunkasiri and Gobwute Rujijanagul

Vol.51 No.6 (November 2024)
Research Article View: 1,934 Download: 375

Ultrathin Polyelectrolyte Multilayer Films with Silver Nanoparticles as a Potential Antibacterial Coating
DOI: 10.12982/CMJS.2024.061.

Meluzvia Marie Amora, Noreen Grace V. Fundador and Erwin Oliver V. Fundador

Vol.51 No.4 (July 2024)
Research Article View: 2,438 Download: 606

Evaluation of Alcoholic Extracts of Mucuna pruriens (L.) DC. Var. utilis for Antibacterial, Antioxidant and Cytotoxic Activities toward Human Cancer Cell Lines
DOI: 10.12982/CMJS.2023.045.

Kanokwan Jitpimai, Lukana Ngiwsara, Weeranuch Lang, Thanawan Panichpat, Ratchanee Mingma, Jisnuson Svasti and Jintanart Wongchawalit

Vol.50 No.5 (September 2023)
Research Article View: 2,699 Download: 1,175

Development of Polycaprolactone Infiltrated Anti-Tuberculosis Drug-Loaded 3D-Printed Hydroxyapatite for Localized and Sustained Drug Release in Bone and Joint Tuberculosis Treatment
DOI: 10.12982/CMJS.2022.009.

Faungchat Thammarakcharoen, Autcharaporn Srion, Watchara Chokevivat, Ruedee Hemstapat, Noppawan Phumala Morales and Jintamai Suwanprateeb

Vol.49 No.1 (Special Issue I : Jan 2022)
Research Article View: 1,611 Download: 489

Fatigue Analysis Model for Applying Bioglass–Hydroxyapatite Biocomposite LCP Bone Fixation Plates to Fix Humeral Shaft Fractures
page: 231 - 251

Siwasit Pitjamit and Wasawat Nakkiew*

Vol.48 No.1 (January 2021)
Research Article View: 1,022 Download: 516

Straightforward Synthesis of (6-methyl-pyridin-2-ylamino)-Acetic Acid from 2-amino-6-methylpyridine and Their Coordination with Copper to Enhance Antibacterial Activity
page: 1265 - 1282

Husna Syaima, Venty Suryanti and Sentot B. Rahardjo

Vol.47 No.6 (November 2020)
Research Article View: 1,201 Download: 485

Low Temperature Fabrication of Brushite by PowderBased Three Dimensional Printing Coupled with Phase Transformation Process
page: 738 - 751

Autcharaporn Srion, Phee Palanuruksa, Faungchat Thammarakcharoen and Jintamai Suwanprateeb*

Vol.47 No.4 (Special Issue II : July 2020)
Research Article View: 1,097 Download: 528

BMP-2 Incorporation into 3D Printed Porous Hydroxyapatite by Rapid Biomimetic Co-Precipitation Technique Using Accelerated Calcium Phosphate Solution
page: 723 - 737

Faungchat Thammarakcharoen and Jintamai Suwanprateeb*

Vol.47 No.4 (Special Issue II : July 2020)
Research Article View: 1,110 Download: 531

Pharmaceutical and Cosmeceutical Biological Activities of Hemp (Cannabis sativa L var. sativa) Leaf and Seed Extracts
page: 180 - 195

Aranya Manosroi, Charinya Chankhampan, Bang-on Kietthanakorn, Warintorn Ruksiriwanich, Puxvadee Chaikul, Korawinwich Boonpisuttinant, Mathukorn Sainakham, Worapaka Manosroi, Theeraphong Tangjai and Jiradej Manosroi

Vol.46 No.2 (March 2019)
Research Article View: 1,933 Download: 661

Ecteinascidin 770, A Tetrahydroisoquinoline Alkaloid, Targeting the Bacterial Cell Division Protein FtsZ
page: 2566 - 2580

Phennapa Charoenwiwattanakij, Jaturong Pratuangdejkul, Montree Jaturanpinyo, Witaya Lowtangkitcharoen, Khanit Suwanborirux and Veena Nukoolkarn

Vol.45 NO.7 (November 2018)
Research Article View: 1,908 Download: 483

Triterpenoid Saponins of Sapotaceae Plants and Their Bioactivities
page: 2534 - 2553

Toshihiro Akihisa*, Masahiko Abe, Jiradej Manosroi and Aranya Manosroi**

Vol.45 NO.7 (November 2018)
Review Article View: 1,106 Download: 583

Effects of Three Drying Treatments on the Polyphenol Content, Antioxidant and Antimicrobial Properties of Syzygium aromaticum Extract
page: 937 - 948

Suat-HianTan* and Md. Rezaul Karim

Vol.45 No.2 (March 2018)
Research Article View: 1,082 Download: 280

Physicochemical Properties of Magnesium-doped Hydroxyapatite Nanoparticles Prepared by Incipient Wetness Impregnation Method
page: 1091 - 1099

Jutharatana Klinkaewnarong, Sirilak Kamonwannasit, Piaw Phatai*

Vol.44 No.3 (July 2017)
Research Article View: 1,085 Download: 339

Preparation of Nano-hydroxyapatite Particles by Ultrasonic Method at 25 kHz Using Natural Rubber Latex as a Templating Agent
page: 320 - 328

Songkot Utara [a,b]*, Jutharatana Klinkaewnarong [a,c]

Vol.43 No.2 (SPECIAL ISSUE 1)
Research Article View: 1,043 Download: 292

Enhancing coating property and storage stability of non-alkoxide sol-gel derived hydroxyapatite coating by incorporating gel retarding agents.
page: 1341 - 1351

Jintamai Suwanprateeb*, Waraporn Suvannapruk, Faungchat Thammarakcharoen and Watchara Chokevivat

Vol.41 No.5/2 (OCTOBER 2014)
Research Article View: 951 Download: 307

Marine Derived Fungi of Peninsular Malaysia-a Biochemical Perspective
page: 894 - 909

Audra Shaleena Paliany, Yasodha Sivasothy, Khalijah Awang, Mohammed Rizman-Idid and Siti Aisyah Alia

Vol.41 No.4 (SPECIAL ISSUE 1)
Research Article View: 1,554 Download: 248

Using Alumina and Zirconia Ceramic Composite in Dogs: a Biocompatibility Study
page: 763 - 774

Korakot Nganvongpanit1,2, Alongkot Phothawan2,3, Waranee Pradit2,4, Sukum Eitssayeam2,3, Jongkolnee

Vol.40 No.4 (OCTOBER 2013)
Research Article View: 2,052 Download: 331

EFFECT OF DEGREE OF ACETYLATION ON IN VITRO BIOCOMPATIBILITY OF ELECTROSPUN CELLULOSE ACETATE-BASED FIBROUS MATRICES
page: 209 - 223

Pitt Supaphol,1,2* Artphop Neamnark,1,2 Pattama Taepaiboon,1,2 and Prasit Pavasant 3

Vol.39 No.2 (APRIL 2012)
Research Article View: 981 Download: 230

Nanocrystalline Hydroxyapatite Powders by a Polymerized Complex Method
page: 243 - 251

Jutharatana Klinkaewnarong, and Santi Maensiri

Vol.37 No.2 (MAY 2010)
Research Article View: 970 Download: 271

Chiang Mai Journal of Science

1,647

Q3 0.80

Q3 1.3

7 days

Outline

Figures and Tables