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Effect of Actinomycin D Isolated From the Cultured Broth of Marine Streptomyces spp. on Cell Division Protein FtsZ


Paper Type 
Contributed Paper
Title 
Effect of Actinomycin D Isolated From the Cultured Broth of Marine Streptomyces spp. on Cell Division Protein FtsZ
Author 
Phennapa Charoenwiwattanakij, Jaturong Pratuangdejkul, Sumet chongruchiroj, Khanit Suwanborirux, Chitti Thawai, Jiraporn Chingunpitak and Veena Satitpatipan
Email 
veena.nuk@mahidol.ac.th
Abstract:
 In the course of our investigation on antibacterial substances with FtsZ inhibitory effect,
actinomycin D was isolated from the ethyl acetate extract of Streptomyces sp. LT3-17. The compound
showed antibacterial activities. The MIC values of actinomycin D against Staphylococcus aureus ATCC
25923, Methicillin-resistant Staphylococcus aureus DMST 20654, Bacillus subtilis ATCC 6633, Escherichia
coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853 were 3.13, 0.39, 0.10, 300.00, and 500.00
μg/mL, respectively. The morphology study of E. coli JW0093 treated with the compound showed
inhibitory effect on cell elongation. The inhibition of FtsZ activity by actinomycin D was shown
as the inhibition of GTPase activity with IC50 value, 20.06 μM. The polymerization of E. coli FtsZ
protein (EcFtsZ) treated with 0.01 μM actinomycin D showed the degree of polymerization ratio was
less than 1.0 indicating the inhibitory potential of actinomycin D on FtsZ polymerization. In silico
study was performed to predict binding mode of actinomycin D into nucleotide binding pocket of
the homology model of EcFtsZ protein. From the results of these experiments, the isolation and
elucidation methods of actinomycin D as well as its novel mechanism as FtsZ inhibitors have been
discovered.
 
Start & End Page 
362 - 377
Received Date 
2019-09-18
Revised Date 
Accepted Date 
2020-02-05
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Keyword 
actinomycin D, antibacterial agent, FtsZ, marine, Streptomyces
Volume 
Vol.47 No.3 (May 2020)
DOI 
Citation