Paper Type |
Contributed Paper |
Title |
Effect of Actinomycin D Isolated From the Cultured Broth of Marine Streptomyces spp. on Cell Division Protein FtsZ |
Author |
Phennapa Charoenwiwattanakij, Jaturong Pratuangdejkul, Sumet chongruchiroj, Khanit Suwanborirux, Chitti Thawai, Jiraporn Chingunpitak and Veena Satitpatipan |
Email |
veena.nuk@mahidol.ac.th |
Abstract: In the course of our investigation on antibacterial substances with FtsZ inhibitory effect,
actinomycin D was isolated from the ethyl acetate extract of Streptomyces sp. LT3-17. The compound
showed antibacterial activities. The MIC values of actinomycin D against Staphylococcus aureus ATCC
25923, Methicillin-resistant Staphylococcus aureus DMST 20654, Bacillus subtilis ATCC 6633, Escherichia
coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853 were 3.13, 0.39, 0.10, 300.00, and 500.00
μg/mL, respectively. The morphology study of E. coli JW0093 treated with the compound showed
inhibitory effect on cell elongation. The inhibition of FtsZ activity by actinomycin D was shown
as the inhibition of GTPase activity with IC50 value, 20.06 μM. The polymerization of E. coli FtsZ
protein (EcFtsZ) treated with 0.01 μM actinomycin D showed the degree of polymerization ratio was
less than 1.0 indicating the inhibitory potential of actinomycin D on FtsZ polymerization. In silico
study was performed to predict binding mode of actinomycin D into nucleotide binding pocket of
the homology model of EcFtsZ protein. From the results of these experiments, the isolation and
elucidation methods of actinomycin D as well as its novel mechanism as FtsZ inhibitors have been
discovered.
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Start & End Page |
362 - 377 |
Received Date |
2019-09-18 |
Revised Date |
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Accepted Date |
2020-02-05 |
Full Text |
Download |
Keyword |
actinomycin D, antibacterial agent, FtsZ, marine, Streptomyces |
Volume |
Vol.47 No.3 (May 2020) |
DOI |
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Citation |